Structural rearrangements in the nucleus localize latent HIV proviruses to a perinucleolar compartment supportive of reactivation

• 成果类型: Article
• 署名作者: Kizito, Fredrick; Nguyen, Kien; Mbonye, Uri; Shukla, Meenakshi; Luttge, Benjamin; Checkley, Mary Ann; Agaponova, Anna; Leskov, Konstantin; Karn, Jonathan
• 署名单位: University System of Ohio; Case Western Reserve University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-12603
• DOI: 10.1073/pnas.2202003121
• 发表日期: 2024-04-26
• 关键词: cd4(+) t-cells; transcription elongation; replication; integration; ORGANIZATION; association; maintenance; reservoir; cancer; cpsf6

摘要

Using an immunofluorescence assay based on CRISPR - dCas9 - gRNA complexes that selectively bind to the HIV LTR (HIV Cas - FISH), we traced changes in HIV DNA localization in primary effector T cells from early infection until the cells become quiescent as they transition to memory cells. Unintegrated HIV DNA colocalized with CPSF6 and HIV capsid (CA, p24) was found in the cytoplasm and nuclear periphery at days 1 and 3 post infection. From days 3 to 7, most HIV DNA was distributed primarily in the nuclear intermediate euchromatic compartment and was transcribed. By day 21, the cells had entered quiescence, and HIV DNA accumulated in the perinucleolar compartment (PNC). The localization of proviruses to the PNC was blocked by integrase inhibitor Raltegravir, suggesting it was due to chromosomal rearrangements. During the reactivation of latently infected cells through the T cell receptor (TCR), nascent viral mRNA transcripts associated with HIV DNA in the PNC were detected. The viral trans - activator Tat and its regulatory partners, P - TEFb and 7SK snRNA, assembled in large interchromatin granule clusters near the provirus within 2 h of TCR activation. As T cell activation progressed, the HIV DNA shifted away from the PNC. HIV DNA in latently infected memory T cells from patients also accumulated in the PNC and showed identical patterns of nuclear rearrangements after cellular reactivation. Thus, in contrast to transformed cells where proviruses are found primarily at the nuclear periphery, in primary memory T cells, the nuclear architecture undergoes rearrangements that shape the and reactivation of HIV.