-
作者：Bian, Hanxiao; Song, Peizhe; Gao, Ying; Deng, Zhiping; Huang, Chenyang; Yu, Lei; Wang, Hanqing; Ye, Bingbing; Cai, Zhihe; Pan, Yu; Wang, Fengqin; Liu, Jianzhao; Gao, Xiangwei; Chen, Kunsong; Jia, Guifang; Klee, Harry J.; Zhang, Bo
作者单位：Universidade Federal de Minas Gerais; Chinese Academy of Sciences; Peking University; Chongqing University; Zhejiang Academy of Agricultural Sciences; Zhejiang University; Zhejiang University; Southwest University - China; Zhejiang University; Peking University; Peking University; Zhejiang University
摘要：N 6- methyladenosine (m 6 A) is a fundamentally important RNA modification for gene regulation, whose function is achieved through m 6 A readers. However, whether and how m 6 A readers play regulatory roles during fruit ripening and quality formation remains unclear. Here, we characterized SlYTH2 as a tomato m 6 A reader protein and profiled the binding sites of SlYTH2 at the transcriptome- wide level. SlYTH2 undergoes liquid- liquid phase separation and promotes RNA-protein condensate formati...
-
作者：McConville, Robyn; Krol, Jelte M. M.; Steel, Ryan W. J.; O'Neill, Matthew T.; Davey, Bethany K.; Hodder, Anthony N.; Nebl, Thomas; Cowman, Alan F.; Kneteman, Norman; Boddey, Justin A.
作者单位：Walter & Eliza Hall Institute; University of Melbourne; University of Alberta
摘要：Plasmodium falciparum causes severe malaria and assembles a protein translocon (PTEX) complex at the parasitophorous vacuole membrane (PVM) of infected erythrocytes, through which several hundred proteins are exported to facilitate growth. The preceding liver stage of infection involves growth in a hepatocyte- derived PVM; however, the importance of protein export during P. falciparum liver infection remains unexplored. Here, we use the FlpL/ FRT system to conditionally excise genes in P. falc...
-
作者：Brenowitz, Eliot A.; Lent, Karin L.; Miller, Kimberly E.; Perkel, David J.
作者单位：University of Washington; University of Washington Seattle; University of Washington; University of Washington Seattle; University of Washington; University of Washington Seattle
摘要：In adult songbirds, new neurons are born in large numbers in the proliferative ventricular zone in the telencephalon and migrate to the adjacent song control region HVC (acronym used as proper name) [A. Reiner et al. , J. Comp. Neurol. 473 , 377-414 (2004)]. Many of these new neurons send long axonal projections to the robust nucleus of the arcopallium (RA). The HVC-RA circuit is essential for producing stereotyped learned song. The function of adult neurogenesis in this circuit has not been c...
-
作者：de la Prada, alex G.; Small, Mario L.
作者单位：University of Bremen; Columbia University
摘要：In US cities, neighborhoods have long been racially segregated. However, people do not spend all their time in their neighborhoods, and the consequences of residential segregation may be tempered by the contact people have with other racial groups as they traverse the city daily. We examine the extent to which people's regular travel throughout the city is to places beyond their comfort zone (BCZ), i.e., to neighborhoods of racial composition different from their own-and why. Based on travel p...
-
作者：Parker, Diana; Tosiani, Anna; Yazid, Muhammad; Sari, Inggit L.; Kartika, Tatik; Kustiyoc; Firmansyah, Rizky; Said, Zuraidah; Wijaya, Arief; Potapov, Peter; Tyukavina, Alexandra; Stehman, Stephen V.; Zalles, Viviana; Pickens, Amy; Pickering, Jeffrey; Turubanova, Svetlana; Hansen, Matthew C.
作者单位：University System of Maryland; University of Maryland College Park; Ministry of Environment & Forestry; National Research & Innovation Agency of Indonesia (BRIN); State University of New York (SUNY) System; State University of New York (SUNY) College of Environmental Science & Forestry
摘要：Indonesia has experienced rapid primary forest loss, second only to Brazil in modern history. We examined the fates of Indonesian deforested areas, immediately after clearing and over time, to quantify deforestation drivers in Indonesia. Using time- series satellite data, we tracked degradation and clearing events in intact and degraded natural forests from 1991 to 2020, as well as land use trajectories after forest loss. While an estimated 7.8 Mha (SE = 0.4) of forest cleared during this peri...
-
作者：Tang, Feng; Wang, Yinan; Zhao, Ting; Yuan, Jun; Wang, Yinsheng
作者单位：University of California System; University of California Riverside; University of California System; University of California Riverside
摘要：Cycling cells replicate their DNA during the S phase through a defined temporal program known as replication timing. Mutation frequencies, epigenetic chromatin states, and transcriptional activities are different for genomic regions that are replicated early and late in the S phase. Here, we found from ChIP- Seq analysis that DNA polymerase (Pol) kappa is enriched in early- replicating genomic regions in HEK293T cells. In addition, by feeding cells with N 2- heptynyl- 2 '- deoxyguanosine follo...
-
作者：Gewurz, Benjamin E.; Mosialos, George; Rickinson, Alan B.; Swaminathan, Sankar
作者单位：Harvard University; Harvard University Medical Affiliates; Brigham & Women's Hospital; Aristotle University of Thessaloniki; University of Birmingham; Utah System of Higher Education; University of Utah
-
作者：Zhang, Deyi; Shao, Yi; Zhou, Jiayi; Zhan, Qiangwei; Wen, Ziyang; Mao, Sheng; Wei, Jingjing; Qi, Limin; Shao, Yuanhua; Wang, Huan
作者单位：Peking University; Peking University; Shandong University
摘要：Liquid- phase electron microscopy (LP- EM) imaging has revolutionized our understanding sion that requires only standard microscopes. Depending on the imaging condition, the open geometry of the nanopipette allows the imaging of evaporation- induced pattern formation, but it can also function as an ordinary closed- geometry liquid cell where evaporation is negligible despite the nano opening. The nano coffee- ring phenomenon was observed by tracking individual nanoparticles in an evaporating n...
-
作者：Gill, Hasreet K.; Yin, Sifan; Lawlor, John C.; Huycke, Tyler R.; Nerurkar, Nandan L.; Tabin, Clifford J.; Mahadevan, L.
作者单位：Harvard University; Harvard Medical School; Harvard University; University of California System; University of California San Francisco; Columbia University; Harvard University; Harvard University
摘要：Tissue buckling is an increasingly appreciated mode of morphogenesis in the embryo, but it is often unclear how geometric and material parameters are molecularly determined in native developmental contexts to generate diverse functional patterns. Here, we study the link between differential mechanical properties and the morphogenesis of distinct anteroposterior compartments in the intestinal tract-the esophagus, small intestine, and large intestine. These regions originate from a simple, commo...
-
作者：Yin, Sheng; Wu, Haipeng; Huang, Yaying; Lu, Chenjing; Cui, Jian; Li, Ying; Xue, Bin; Wu, Junhua; Jiang, Chunping; Gu, Xiaosong; Wang, Wei; Cao, Yi
作者单位：Nanjing University; Collaborative Innovation Center of Advanced Microstructures (CICAM); Jinan Microecological Biomedicine Shandong Laboratory; Nanjing University of Information Science & Technology; Nanjing University; Nanjing University; Nanjing University; Nanjing University; Nanjing University
摘要：Mesenchymal stem cells (MSCs) are essential in regenerative medicine. However, conventional expansion and harvesting methods often fail to maintain the essential extracellular matrix (ECM) components, which are crucial for their functionality and efficacy in therapeutic applications. Here, we introduce a bone marrow- inspired macroporous hydrogel designed for the large- scale production of MSC-ECM spheroids. Through a soft- templating approach leveraging liquid-liquid phase separation, we engi...
