The m6A reader SlYTH2 negatively regulates tomato fruit aroma by impeding the translation process

• 成果类型: Article
• 署名作者: Bian, Hanxiao; Song, Peizhe; Gao, Ying; Deng, Zhiping; Huang, Chenyang; Yu, Lei; Wang, Hanqing; Ye, Bingbing; Cai, Zhihe; Pan, Yu; Wang, Fengqin; Liu, Jianzhao; Gao, Xiangwei; Chen, Kunsong; Jia, Guifang; Klee, Harry J.; Zhang, Bo
• 署名单位: Universidade Federal de Minas Gerais; Chinese Academy of Sciences; Peking University; Chongqing University; Zhejiang Academy of Agricultural Sciences; Zhejiang University; Zhejiang University; Southwest University - China; Zhejiang University; Peking University; Peking University; Zhejiang University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-10001
• DOI: 10.1073/pnas.2405100121
• 发表日期: 2024-07-09
• 关键词: yth domain; flavor volatiles; rna; RECOGNITION; stress; polyadenylation; identification; granules; tomloxc; FAMILY

摘要

N 6- methyladenosine (m 6 A) is a fundamentally important RNA modification for gene regulation, whose function is achieved through m 6 A readers. However, whether and how m 6 A readers play regulatory roles during fruit ripening and quality formation remains unclear. Here, we characterized SlYTH2 as a tomato m 6 A reader protein and profiled the binding sites of SlYTH2 at the transcriptome- wide level. SlYTH2 undergoes liquid- liquid phase separation and promotes RNA-protein condensate formation. The target mRNAs of SlYTH2, namely m 6 A- modified SlHPL and SlCCD1B associated with volatile synthesis, are enriched in SlYTH2- induced condensates. Through polysome profiling assays and proteomic analysis, we demonstrate that knockout of SlYTH2 expedites the translation process of SlHPL and SlCCD1B , resulting in augmented production of aroma- associated volatiles. This aroma enrichment significantly increased consumer preferences for CRISPR- edited fruit over wild type. These findings shed light on the underlying mechanisms of m 6 A in plant RNA metabolism and provided a promising strategy to generate fruits that are more attractive to consumers.