34期

最新文章

• Uncovering underlying physical principles and driving forces of cell differentiation and reprogramming from single-cell transcriptomics AI翻译
  作者：Zhu, Ligang; Yang, Songlin; Zhang, Kun; Wang, Hong; Fang, Xiaona; Wang, Jin
  作者单位：Jilin University; Chinese Academy of Sciences; Changchun Institute of Applied Chemistry, CAS; Northeast Normal University - China; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; State University of New York (SUNY) System; Stony Brook University
  摘要：Recent advances in single-cell sequencing technology have revolutionized our ability to acquire whole transcriptome data. However, uncovering the underlying transcriptional drivers and nonequilibrium driving forces of cell function directly from these data remains challenging. We address this by learning cell state vector fields from discrete single-cell RNA velocity to quantify the single-cell global nonequilibrium driving forces as landscape and flux. From single-cell data, we quantified the...
• Vulnerability in research ethics: A call for assessing vulnerability and implementing protections AI翻译
  作者：Findley, Michael G.; Ghosn, Faten; Lowe, Sara J.
  作者单位：University of Texas System; University of Texas Austin; University of Essex; University of Texas System; University of Texas Austin
  摘要：Ethics standards reference the need for special consideration of vulnerable populations, such as pregnant women, incarcerated individuals, and minors. The concept of vulnerability is poorly conceptualized in the medical sciences where it originated, and its application to the social sciences is even more challenging. Social science researchers may unwittingly fail to appreciate preexisting vulnerabilities and indeed may be responsible for inducing new research-related vulnerability. In this pa...
• Zika virus NS5 protein inhibits type I interferon signaling via CRL3 E3 ubiquitin ligase- mediated degradation of STAT2 AI翻译
  作者：Ren, Wenlin; Fu, Chonglei; Zhang, Yu; Ju, Xiaohui; Jiang, Xi; Song, Jingwei; Gong, Mingli; Li, Zhuoyang; Fan, Wenchun; Yao, Jun; Ding, Qiang
  作者单位：Tsinghua University; Tsinghua University; Shanxi Medical University; Shanxi Medical University; Zhejiang University
  摘要：The ZIKA virus (ZIKV) evades the host immune response by degrading STAT2 through its NS5 protein, thereby inhibiting type I interferon (IFN)- mediated antiviral immunity. However, the molecular mechanism underlying this process has remained elusive. In this study, we performed a genome-wide CRISPR/Cas9 screen, revealing that ZSWIM8 as the substrate receptor of Cullin3-RING E3 ligase is required for NS5- mediated STAT2 degradation. Genetic depletion of ZSWIM8 and CUL3 substantially impeded NS5-...
• Light regulates widespread plant alternative polyadenylation through the chloroplast AI翻译
  作者：Kubaczka, M. Guillermina; Herz, Micaela A. Godoy; Chen, Wei- Chun; Zheng, Dinghai; Petrillo, Ezequiel; Tan, Bin; Kornblihtt, Alberto R.
  作者单位：University of Buenos Aires; Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET); University of Buenos Aires; CONICET UBA; Rutgers University System; Rutgers University New Brunswick; Rutgers University Biomedical & Health Sciences; The Wistar Institute; The Wistar Institute
  摘要：Transcription of eukaryotic protein- coding genes generates immature mRNAs that are subjected to a series of processing events, including capping, splicing, cleavage, and polyadenylation (CPA), and chemical modifications of bases. Alternative polyadenylation (APA) greatly contributes to mRNA diversity in the cell. By determining the length of the 3' untranslated region, APA generates transcripts with different regulatory elements, such as miRNA and RBP binding sites, which can influence mRNA s...
• IL-13 and IL-17A activate β1 integrin through an NF- kB/Rho kinase/PIP5K1γ pathway to enhance force transmission in airway smooth muscle AI翻译
  作者：Ngo, Uyen; Shi, Ying; Woodruff, Prescott; Shokat, Kevan; Degrado, William; Jo, Hyunil; Sheppard, Dean; Sundaram, Aparna B.
  作者单位：University of California System; University of California San Francisco; University of California System; University of California San Francisco; University of California System; University of California San Francisco; University of California System; University of California San Francisco; Howard Hughes Medical Institute; University of California System; University of California San Francisco; University of California System; University of California San Francisco
  摘要：Integrin activation resulting in enhanced adhesion to the extracellular matrix plays a key role in fundamental cellular processes. Although integrin activation has been extensively studied in circulating cells such as leukocytes and platelets, much less is known about the regulation and functional impact of integrin activation in adherent cells such as smooth muscle. Here, we show that two different asthmagenic cytokines, IL- 13 and IL- 17A, activate type I and IL- 17 cytokine receptor familie...
• Zfp697 is an RNA- binding protein that regulates skeletal muscle inflammation and remodeling AI翻译
  作者：Correia, Jorge C.; Jannig, Paulo R.; Gosztyla, Maya L.; Cervenka, Igor; Ducommun, Serge; Praestholm, Stine M.; Dias, Jose M.; Dumont, Kyle D.; Liu, Zhengye; Liang, Qishan; Edsgaerd, Daniel; Emanuelsson, Olof; Gregorevic, Paul; Westerblad, Hakan; Venckunas, Tomas; Brazaitis, Marius; Kamandulis, Sigitas; Lanner, Johanna T.; Teixeira, Ana I.; Yeo, Gene W.; Ruas, Jorge L.
  作者单位：Karolinska Institutet; University of California System; University of California San Diego; University of California System; University of California San Diego; University of California System; University of California San Diego; Karolinska Institutet; Karolinska Institutet; Karolinska Institutet; University of California System; University of California San Diego; Royal Institute of Technology; University of Melbourne; Karolinska Institutet; Lithuanian Sports University; University of Michigan System; University of Michigan; University of Michigan System; University of Michigan
  摘要：Skeletal muscle atrophy is a morbidity and mortality risk factor that happens with disuse, chronic disease, and aging. The tissue remodeling that happens during recovery from atrophy or injury involves changes in different cell types such as muscle fibers, and satellite and immune cells. Here, we show that the previously uncharacterized gene and protein Zfp697 is a damage- induced regulator of muscle remodeling. Zfp697/ ZNF697 expression is transiently elevated during recovery from muscle atro...
• DNA- DISK: Automated end- to- end data storage via enzymatic single- nucleotide DNA synthesis and sequencing on digital microfluidics AI翻译
  作者：Li, Kunjie; Lu, Xiaoyun; Liao, Jiaqi; Chen, Heng; Lin, Wei; Zhao, Yuhan; Tang, Dongbao; Li, Congyu; Tian, Zhenyang; Zhu, Zhi; Jiang, Huifeng; Sun, Jun; Zhang, Huimin; Yang, Chaoyong
  作者单位：Xiamen University; Chinese Academy of Sciences; Tianjin Institute of Industrial Biotechnology, CAS
  摘要：In the age of information explosion, the exponential growth of digital data far exceeds the capacity of current mainstream storage media. DNA is emerging as a promising alternative due to its higher storage density, longer retention time, and lower power consumption. To date, commercially mature DNA synthesis and sequencing technologies allow for writing and reading of information on DNA with customization and convenience at the research level. However, under the disconnected and nonspecialize...
• Rtt105 stimulates Rad51-ssDNA assembly and orchestrates Rad51 and RPA actions to promote homologous recombination repair AI翻译
  作者：Wang, Xuejie; Zhao, Xiaocong; Yu, Zhengshi; Fan, Tianai; Guo, Yunjing; Liang, Jianqiang; Wang, Yanyan; Zhan, Jingfei; Chen, Guifang; Zhou, Chun; Zhang, Xinghua; Li, Xiangpan; Chen, Xuefeng
  作者单位：Wuhan University; Zhejiang University
  摘要：Homologous recombination (HR) is essential for the maintenance of genome stability. During HR, Replication Protein A (RPA) rapidly coats the 3 '- tailed single- strand DNA (ssDNA) generated by end resection. Then, the ssDNA-bound RPA must be timely replaced by Rad51 recombinase to form Rad51 nucleoprotein filaments that drive homology search and HR repair. How cells regulate Rad51 assembly dynamics and coordinate RPA and Rad51 actions to ensure proper HR remains poorly understood. Here, we ide...
• Capturing RAS oligomerization on a membrane AI翻译
  作者：Yun, Sangho D.; Scott, Elena; Chang, Ding - Yuan; Bahramimoghaddam, Hanieh; Lynn, Michael; Lantz, Carter; Russell, David H.; Laganowsky, Arthur
  作者单位：Texas A&M University System; Texas A&M University College Station
  摘要：RAS GTPases associate with the biological membrane where they function as molecular switches to regulate cell growth. Recent studies indicate that RAS proteins oligomerize on membranes, and disrupting these assemblies represents an alternative therapeutic strategy. However, conflicting reports on RAS assemblies, ranging in size from dimers to nanoclusters, have brought to the fore key questions regarding the stoichiometry and parameters that influence oligomerization. Here, we probe three isof...
• Increasing environmental variability inhibits evolutionary rescue in a long- lived vertebrate AI翻译
  作者：Clark-Wolf, T. J.; Boersma, P. Dee; Plard, Floriane; Rebstock, Ginger A.; Abrahms, Briana
  作者单位：University of Washington; University of Washington Seattle; Utah System of Higher Education; Utah State University
  摘要：Evolutionary rescue, whereby adaptive evolutionary change rescues populations from extinction, is theorized to enable imperiled animal populations to persist under increasing anthropogenic change. Despite a large body of evidence in theoretical and laboratory settings, the potential for evolutionary rescue to be a viable adaptation process for free- ranging animals remains unknown. Here, we leverage a 38- year dataset following the fates of 53,959 Magellanic penguins ( Spheniscus magellanicus)...