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作者：Fernandez- Fernandez, Rocio; Olivenza, David R.; Weyer, Esther; Singh, Abhyudai; Casadesus, Josep; Sanchez-Romero, Maria Antonia
作者单位：University of Sevilla; University of Sevilla; University of Delaware; University of Delaware
摘要：Epigenetic mechanisms can generate bacterial lineages capable of spontaneously switching between distinct phenotypes. Currently, mathematical models and simulations propose epigenetic switches as a mechanism of adaptation to deal with fluctuating environments. However, bacterial evolution experiments for testing these predictions are lacking. Here, we exploit an epigenetic switch in Salmonella enterica, the opvAB operon, to show clear evidence that OpvAB bistability persists in changing enviro...
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作者：Lin, Guowu; Barnes, Christopher O.; Weiss, Simon; Dutagaci, Bercem; Qiu, Chenxi; Feig, Michael; Song, Jihnu; Lyubimov, Artem; Cohen, Aina E.; Kaplan, Craig D.; Calero, Guillermo
作者单位：Pennsylvania Commonwealth System of Higher Education (PCSHE); University of Pittsburgh; California Institute of Technology; Michigan State University; Harvard University; Harvard Medical School; Stanford University; United States Department of Energy (DOE); SLAC National Accelerator Laboratory; Pennsylvania Commonwealth System of Higher Education (PCSHE); University of Pittsburgh; Stanford University; University of California System; University of California Merced
摘要：Catalysis and translocation of multisubunit DNA-directed RNA polymerases underlie all cellular mRNA synthesis. RNA polymerase II (Pol II) synthesizes eukaryotic pre-mRNAs from a DNA template strand buried in its active site. Structural details of catalysis at near-atomic resolution and precise arrangement of key active site components have been elusive. Here, we present the free-electron laser (FEL) structures of a matched ATP-bound Pol II and the hyperactive Rpb1 T834P bridge helix (BH) mutan...
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作者：Zhu, Changan; Hu, Zhangjian; Hu, Chaoyi; Ma, Hongxue; Zhou, Jie; Xia, Xiaojian; Shi, Kai; Foyer, Christine H.; Yu, Jingquan; Zhou, Yanhong
作者单位：Zhejiang University; Zhejiang University; Zhejiang University; University of Birmingham
摘要：ELONGATED HYPOCOTOYL5 (HY5) and PHYTOCHROME INTERACTING FACTORs (PIFs) are two types of important light- related regulators of plant growth, however, their interplay remains elusive. Here, we report that the activated tomato ( Solanum lycopersicum) HY5 (SlHY5) triggers the transcription of a Calcium- dependent Protein Kinase SlCPK27. SlCPK27 interacts with and phosphorylates SlPIF4 at Ser-252 and Ser-308 phosphosites to promote its degradation. SlPIF4 promotes hypocotyl elongation mainly by ac...
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作者：Badoni, Saurabh; Pasion-Uy, Erstelle A.; Kor, Sakshi; Kim, Sung-Ryul; Tiozon, Rhowell N.; Misra, Gopal; Buenafe, Reuben James Q.; Labarga, Luster May; Ramos-Castrosanto, Ana Rose; Pratap, Vipin; Slamet-Loedin, Inez; von Steimker, Julia; Alseekh, Saleh; Fernie, Alisdair R.; Kohli, Ajay; Khush, Gurudev S.; Sreenivasulu, Nese
作者单位：CGIAR; International Rice Research Institute (IRRI); Max Planck Society; University of California System; University of California Davis
摘要：To counter the rising incidence of diabetes and to meet the daily protein needs, we created low glycemic index (GI) rice varieties with protein content (PC) surpassing amylose extender (IR36ae) as parental lines, we identified quantitative trait loci and genes associated with low GI, high amylose content (AC), and high PC. By integrating genetic techniques with classification models, this comprehensive approach identified candidate genes on chromosome 2 (qGI2.1/qAC2.1 spanning the region from ...
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作者：Livraghi, Luca; Hanly, Joseph J.; Evans, Elizabeth; Wright, Charlotte J.; Loh, Ling S.; Mazo-Vargas, Anyi; Kamrava, Kiana; Carter, Alexander; van der Heijden, Eva S. M.; Reed, Robert D.; Papa, Riccardo; Jiggins, Chris D.; Martin, Arnaud
作者单位：George Washington University; University of Cambridge; Duke University; Smithsonian Institution; Smithsonian Tropical Research Institute; University of Puerto Rico; University of Puerto Rico Rio Piedras; Wellcome Trust Sanger Institute; Cornell University; University of Puerto Rico; University of Puerto Rico Medical Sciences Campus; University of Puerto Rico; University of Parma
摘要：Evolutionary variation in the wing pigmentation of butterflies and moths offers striking examples of adaptation by crypsis and mimicry. The cortex locus has been independently mapped as the locus controlling color polymorphisms in 15 lepidopteran species, suggesting that it acts as a genomic hotspot for the diversification of wing patterns, but functional validation through protein- coding knockouts has proven difficult to obtain. Our study unveils the role of a long noncoding RNA (lncRNA) whi...
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作者：Su, Xiaofeng A.; Stopsack, Konrad H.; Schmidt, Daniel R.; Ma, Duanduan; Li, Zhe; Scheet, Paul A.; Penney, Kathryn L.; Lotan, Tamara L.; Abida, Wassim; DeArment, Elise G.; Lu, Kate; Janas, Thomas; Hu, Sofia; Vander Heiden, Matthew G.; Loda, Massimo; Boselli, Monica; Amon, Angelika; Mucci, Lorelei A.
作者单位：Massachusetts Institute of Technology (MIT); Massachusetts Institute of Technology (MIT); Uniformed Services University of the Health Sciences - USA; Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI); Harvard University; Harvard T.H. Chan School of Public Health; Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; Harvard University; Harvard Medical School; Harvard University; Harvard University Medical Affiliates; Beth Israel Deaconess Medical Center; Harvard University; Harvard Medical School; Massachusetts Institute of Technology (MIT); Harvard University; Harvard University Medical Affiliates; Brigham & Women's Hospital; Harvard University; Harvard Medical School; University of Texas System; UTMD Anderson Cancer Center; Johns Hopkins University; Memorial Sloan Kettering Cancer Center; Harvard University; Harvard University Medical Affiliates; Dana-Farber Cancer Institute; Howard Hughes Medical Institute; American Cancer Society
摘要：Higher levels of aneuploidy, characterized by imbalanced chromosome numbers, are associated with lethal progression in prostate cancer. However, how aneuploidy contributes to prostate cancer aggressiveness remains poorly understood. In this study, we assessed in patients which genes on chromosome 8q, one of the most frequently gained chromosome arms in prostate tumors, were most strongly associated with long- term risk of cancer progression to metastases and death from prostate cancer (lethal ...
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作者：Fujiwara, Yuko; Takahashi, Ryou-u; Saito, Motonobu; Umakoshi, Michinobu; Shimada, Yoko; Koyama, Kei; Yatabe, Yasushi; Watanabe, Shun-ichi; Koyota, Souichi; Minamiya, Yoshihiro; Tahara, Hidetoshi; Kono, Koji; Shiraishi, Kouya; Kohno, Takashi; Goto, Akiteru; Tsuchiya, Naoto
作者单位：National Cancer Center - Japan; Hiroshima University; Fukushima Medical University; Akita University; National Cancer Center - Japan; National Cancer Center - Japan; National Cancer Center - Japan; Akita University; Akita University; National Cancer Center - Japan
摘要：The nature of microRNA (miRNA) dysfunction in carcinogenesis remains controversial because of the complex connection between miRNA structural diversity and biological processes. Here, we found that oncofetal IGF2BP3 regulates the selective production of a subset of 3 '- isoforms (3 '- isomiRs), including miR-21-5p and Let- 7 family, which induces significant changes in their cellular seed occupancy and structural components, establishing a cancer- specific gene expression profile. The D- score...
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作者：Ho, Christine; Jutras-Dube, Laurent; Zhao, Michael L.; Moenke, Gregor; Kiss, Istvan Z.; Francois, Paul; Aulehla, Alexander
作者单位：European Molecular Biology Laboratory (EMBL); McGill University; Saint Louis University; Washington University (WUSTL); University of Southern California; Rockefeller University; Universite de Montreal
摘要：Synchronization of coupled oscillators is a universal phenomenon encountered across different scales and contexts, e.g., chemical wave patterns, superconductors, and the unison applause we witness in concert halls. The existence of common underlying coupling rules defines universality classes, revealing a fundamental sameness between seemingly distinct systems. Identifying rules of synchronization in any particular setting is hence of paramount relevance. Here, we address the coupling rules wi...
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作者：Nagae, Takayuki; Takeda, Mitsuhiro; Noji, Tomoyasu; Saito, Keisuke; Aoyama, Hiroshi; Miyanoiri, Yohei; Ito, Yutaka; Kainosho, Masatsune; Hirose, Yuu; Ishikita, Hiroshi; Mishima, Masaki
作者单位：Tokyo University of Pharmacy & Life Sciences; University of Tokyo; University of Tokyo; University of Osaka; Tokyo Metropolitan University; Toyohashi University of Technology
摘要：Deprotonation or suppression of the pKa of the amino group of a lysine sidechain is a widely recognized phenomenon whereby the sidechain amino group transiently can act as a nucleophile at the active site of enzymatic reactions. However, a deprotonated lysine and its molecular interactions have not been directly experimentally detected. Here, we demonstrate a deprotonated lysine stably serving as an acceptor in a H- bond between the photosensor protein RcaE and its chromophore. Signal splittin...
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作者：Wang, Xiaolei; Dou, Ying; Hu, Jingchu; Chan, Celia Hoi- Ching; Li, Renhao; Rong, Li; Gong, Huarui; Deng, Jian; Yuen, Terrence Tsz- Tai; Lin, Xuansheng; He, Yige; Su, Canhui; Zhang, Bao - Zhong; Chan, Jasper Fuk- Woo; Yuen, Kwok - Yung; Chu, Hin; Huang, Jian - Dong
作者单位：University of Hong Kong; University of Hong Kong; University of Hong Kong; Chinese Academy of Sciences; Shenzhen Institute of Advanced Technology, CAS; Shandong University; University of Hong Kong; University of Hong Kong; University of Hong Kong; Sun Yat Sen University; University of Hong Kong
摘要：Developing an effective Staphylococcus aureus (S. aureus) vaccine has been a challenging endeavor, as demonstrated by numerous failed clinical trials over the years. In this study, we formulated a vaccine containing a highly conserved moonlighting protein, the pyruvate dehydrogenase complex E2 subunit (PDHC), and showed that it induced strong protective immunity against epidemiologically relevant staphylococcal strains in various murine disease models. While antibody responses contributed to b...
