SlCPK27 cross- links SlHY5 and SlPIF4 in brassinosteroiddependent photo- and thermo- morphogenesis in tomato

• 成果类型: Article
• 署名作者: Zhu, Changan; Hu, Zhangjian; Hu, Chaoyi; Ma, Hongxue; Zhou, Jie; Xia, Xiaojian; Shi, Kai; Foyer, Christine H.; Yu, Jingquan; Zhou, Yanhong
• 署名单位: Zhejiang University; Zhejiang University; Zhejiang University; University of Birmingham
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8828
• DOI: 10.1073/pnas.2403040121
• 发表日期: 2024-09-03
• 关键词: plant-growth; light; pif4; phosphorylation; biosynthesis; arabidopsis; elongation; hy5

摘要

ELONGATED HYPOCOTOYL5 (HY5) and PHYTOCHROME INTERACTING FACTORs (PIFs) are two types of important light- related regulators of plant growth, however, their interplay remains elusive. Here, we report that the activated tomato ( Solanum lycopersicum) HY5 (SlHY5) triggers the transcription of a Calcium- dependent Protein Kinase SlCPK27. SlCPK27 interacts with and phosphorylates SlPIF4 at Ser-252 and Ser-308 phosphosites to promote its degradation. SlPIF4 promotes hypocotyl elongation mainly by activating the transcription of SlDWF, a key gene in brassinosteroid (BR) biosynthesis. Such a SlHY5- SlCPK27- SlPIF4-BR cascade not only plays a crucial role in photomorphogenesis but also regulates thermomorphogenesis. Our results uncover a previously unidentified mechanism that integrates Ca2+ signaling with the light signaling pathways to regulate plant growth by modulating BR biosynthesis in response to changes in ambient light and temperature.