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作者：Ma, Xiqi; Zhang, Zhixiong; Barba-Bon, Andrea; Han, Dongxue; Qi, Zichun; Ge, Baosheng; He, Hua; Huang, Fang; Nau, Werner M.; Wang, Xiaojuan
作者单位：China University of Petroleum; Constructor University
摘要：Intracellular protein delivery has the potential to revolutionize cell- biological research and medicinal therapy, with broad applications in bioimaging, disease treatment, and genome editing. Herein, we demonstrate successful delivery of a functional protein, cytochrome c (CYC), by using a boron cluster anion as molecular carrier of the superchaotropic anion type (B12Br11OPr2-). CYC was delivered into lipid bilayer vesicles as well as living cells, with a cellular uptake ratio approaching 90%...
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作者：Stafl, Krystof; Travnicek, Martin; Janovska, Anna; Kucerova, Dana; Pecnova, Lubomira; Yang, Zhiqi; Stepanec, Vladimir; Jech, Lukas; Salker, Madhuri S.; Hejnar, Jiri; Trejbalova, Katerina
作者单位：Czech Academy of Sciences; Institute of Molecular Genetics of the Czech Academy of Sciences; Czech Academy of Sciences; Institute of Molecular Genetics of the Czech Academy of Sciences; Eberhard Karls University of Tubingen
摘要：Syncytin-1, a human fusogenic protein of retroviral origin, is crucial for placental syncytiotrophoblast formation. To mediate cell- to- cell fusion, Syncytin-1 requires specific interaction with its cognate receptor. Two trimeric transmembrane proteins, Alanine, Serine, Cysteine Transporters 1 and 2 (ASCT1 and ASCT2), were suggested and widely accepted as Syncytin-1 cellular receptors. To quantitatively assess the individual contributions of human ASCT1 and ASCT2 to the fusogenic activity of ...
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作者：Taylor, Isaiah W.; Patharkar, O. Rahul; Mijar, Medhavinee; Hsu, Che-Wei; Baer, John; Niederhuth, Chad E.; Ohler, Uwe; Benfey, Philip N.; Walker, John C.
作者单位：Duke University; Washington University (WUSTL); Michigan State University; Helmholtz Association; Max Delbruck Center for Molecular Medicine; University of Missouri System; University of Missouri Columbia
摘要：Abscission is the programmed separation of plant organs. It is widespread in the plant kingdom with important functions in development and environmental response. In Arabidopsis, abscission of floral organs (sepals, petals, and stamens) is controlled by two receptor- like protein kinases HAESA (HAE) and HAESA LIKE- 2 (HSL2), which orchestrate the programmed dissolution of the abscission zone connecting floral organs to the developing fruit. In this work, we use single- cell RNA sequencing to c...
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作者：Oguro-Igashira, Eri; Murakami, Mari; Mori, Ryota; Kuwahara, Ryuichi; Kihara, Takako; Kohara, Masaharu; Fujiwara, Makoto; Motooka, Daisuke; Okuzaki, Daisuke; Arase, Mitsuru; Toyota, Hironobu; Peng, Siyun; Ogino, Takayuki; Kitabatake, Yasuji; Morii, Eiichi; Hirota, Seiichi; Ikeuchi, Hiroki; Umemoto, Eiji; Kumanogoh, Atsushi; Takeda, Kiyoshi
作者单位：University of Osaka; University of Osaka; University of Osaka; University of Osaka; Hyogo Medical University; Hyogo Medical University; University of Osaka; University of Osaka; University of Osaka; University of Osaka; University of Osaka; University of Osaka; University of Shizuoka; University of Osaka
摘要：The intestinal lumen is rich in gut microbial metabolites that serve as signaling molecules for gut immune cells. G- protein- coupled receptors (GPCRs) sense metabolites and can act as key mediators that translate gut luminal signals into host immune responses. However, the impacts of gut microbe-GPCR interactions on human physiology have not been fully elucidated. Here, we show that GPR31, which is activated by the gut bacterial metabolite pyruvate, is specifically expressed on type 1 convent...
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作者：Arisco, Nicholas J.; Peterka, Cassio; Diniz, Cesar; Singer, Burton H.; Castro, Marcia C.
作者单位：Harvard University; Harvard T.H. Chan School of Public Health; State University System of Florida; University of Florida
摘要：Ecological change in the Brazilian Amazon is closely linked to human mobility and health. Mining, agriculture, logging, and other activities alter highly diverse ecological and demographic contexts and subsequent exposure to diseases such as malaria. Studies that have attempted to quantify the impact of deforestation on malaria in the Brazilian Amazon have produced conflicting results. However, they varied in methodology and data sources. Most importantly, all studies used annual data, neglect...
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作者：Cowie, Megan; Constantinou, Procopios C.; Curson, Neil J.; Stock, Taylor J. Z.; Grutter, Peter
作者单位：McGill University; University of London; University College London; University of London; University College London
摘要：We use electrostatic force microscopy to spatially resolve random telegraph noise at the Si/SiO2 interface. Our measurements demonstrate that two-state fluctuations are localized at interfacial traps, with bias-dependent rates and amplitudes. These two-level systems lead to correlated carrier number and mobility fluctuations with a range of characteristic timescales; taken together as an ensemble, they give rise to a 1/f power spectral trend. Such individual defect fluctuations at the Si/SiO2 ...
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作者：Azar, Dany
作者单位：Chinese Academy of Sciences
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作者：Poveda-Cuevas, Sergio Alejandro; Lohachova, Kateryna; Markusic, Borna; Dikic, Ivan; Hummer, Gerhard; Bhaskara, Ramachandra M.
作者单位：Goethe University Frankfurt; Goethe University Frankfurt; Max Planck Society; Goethe University Frankfurt
摘要：Intrinsically disordered regions (IDRs) play a pivotal role in organellar remodeling. They transduce signals across membranes, scaffold signaling complexes, and mediate vesicular traffic. Their functions are regulated by constraining conformational ensembles through specific intra- and intermolecular interactions, physical tethering, and posttranslational modifications. The endoplasmic reticulum (ER)-phagy receptor FAM134B/RETREG1, known for its reticulon homology domain (RHD), includes a subs...
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作者：Butowska-Buczynska, Ewa; Klis, Paulina; Zawadzka, Katarzyna; Hanczakowski, Maciej
作者单位：SWPS University of Social Sciences & Humanities; Adam Mickiewicz University
摘要：Current research on memory indicates that learning is most effective when it involves spaced retrieval practice of study materials. Here, we investigated whether the benefits of both retrieval practice and spacing can be further boosted when variability is introduced across practice sessions. Across six experiments, participants learned translations of foreign vocabulary, with foreign words embedded in contextual sentences hinting at the meaning of these words. These sentences were then either...
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作者：Zhu, Qiang; Li, Jingchao; Sun, Haofan; Fan, Zhiya; Hu, Jiating; Chai, Siyuan; Lin, Bingyi; Wu, Liming; Qin, Weijie; Wang, Yong; Hsieh-Wilson, Linda C.; Yi, Wen
作者单位：Zhejiang University; Zhejiang University; Zhejiang University; California Institute of Technology
摘要：Aerobic glycolysis and immune evasion are two key hallmarks of cancer. However, how these two features are mechanistically linked to promote tumor growth is not well understood. Here, we show that the glycolytic enzyme enolase-1 (ENO1) is dynamically modified with an O- linked beta-N- acetylglucosamine (O- GlcNAcylation), and simultaneously regulates aerobic glycolysis and immune evasion via differential glycosylation. Glycosylation of threonine 19 (T19) on ENO1 promotes its glycolytic activit...
