Intrinsically disordered region amplifies membrane remodeling to augment selective ER-phagy

• 成果类型: Article
• 署名作者: Poveda-Cuevas, Sergio Alejandro; Lohachova, Kateryna; Markusic, Borna; Dikic, Ivan; Hummer, Gerhard; Bhaskara, Ramachandra M.
• 署名单位: Goethe University Frankfurt; Goethe University Frankfurt; Max Planck Society; Goethe University Frankfurt
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-14376
• DOI: 10.1073/pnas.2408071121
• 发表日期: 2024-10-29
• 关键词: molecular-dynamics; force-field; protein; martini; RECOGNITION; transitions; generation; fission

摘要

Intrinsically disordered regions (IDRs) play a pivotal role in organellar remodeling. They transduce signals across membranes, scaffold signaling complexes, and mediate vesicular traffic. Their functions are regulated by constraining conformational ensembles through specific intra- and intermolecular interactions, physical tethering, and posttranslational modifications. The endoplasmic reticulum (ER)-phagy receptor FAM134B/RETREG1, known for its reticulon homology domain (RHD), includes a substantial C-terminal IDR housing the LC3 interacting motif. Beyond engaging the autophagic machinery, the function of the FAM134B-IDR is unclear. Here, we investigate the characteristics of the FAM134B-IDR by extensive modeling and molecular dynamics simulations. We present detailed structural models for the IDR, mapping its conformational landscape in solution and membrane-anchored configurations. Our analysis reveals that depending on the membrane anchor, the IDRs collapse onto the membrane and induce positive membrane curvature to varying degrees. The charge patterns underlying this Janus-like behavior are conserved across other ER-phagy receptors. We found that IDRs alone are sufficient to sense curvature. When combined with RHDs, they intensify membrane remodeling and drive efficient protein clustering, leading to faster budding, thereby amplifying RHD remodeling functions. Our simulations provide a perspective on IDRs of FAM134B, their Janus-like membrane interactions, and the resulting modulatory functions during large-scale ER remodeling.