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作者：Cardenas-Benitez, Braulio; Hurtado, Richard; Luo, Xuhao; Lee, Abraham P.
作者单位：University of California System; University of California Irvine; University of California System; University of California Irvine; University of California System; University of California Irvine
摘要：Fast, nondestructive three-dimensional (3D) imaging of live suspension cells remains challenging without substrate treatment or fixation, precluding scalable single-cell morphometry with minimal alterations. While optical sectioning techniques achieve 3D live cell imaging, lateral versus depth resolution differences further complicate analysis. We present a scalable microfluidic method capable of 3D fluorescent isotropic imaging of live, nonadherent cells suspended inside picoliter droplets wi...
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作者：Cochrane, Wesley G.; Bare, Grant A. L.; Joyce, Gerald F.; Horning, David P.
作者单位：Salk Institute
摘要：An RNA ligase ribozyme that catalyzes the joining of RNA molecules of the opposite chiral handedness was optimized for the ability to synthesize its own enantiomer from two component fragments. The mirror- image D- and L- ligases operate in concert to provide a system for cross- chiral replication, whereby they catalyze each other's synthesis and undergo mutual amplification at constant temperature, with apparent exponential growth and a doubling time of about 1 h. Neither the D- nor the L- RN...
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作者：Michel, Sylvia Englund; Lan, Xin; Miller, John; Tans, Pieter; Clark, J. Reid; Schaefer, Hinrich; Sperlich, Peter; Brailsford, Gordon; Morimoto, Shinji; Moossen, Heiko; Li, Jianghanyang
作者单位：University of Colorado System; University of Colorado Boulder; University of Colorado System; University of Colorado Boulder; University of Colorado System; University of Colorado Boulder; National Oceanic Atmospheric Admin (NOAA) - USA; National Institute of Water & Atmospheric Research (NIWA) - New Zealand; Tohoku University; Max Planck Society
摘要：The growth rate of the atmospheric abundance of methane (CH4) reached a record high of 15.4 ppb yr-1 between 2020 and 2022, but the mechanisms driving the accelerated CH4 growth have so far been unclear. In this work, we use measurements of the 13C:12C Network and a box model to investigate potential drivers for the rapid CH4 growth. These measurements show that the record- high CH4 growth in 2020-2022 was accompanied by a sharp decline in S13CCH4, indicating that the increase in CH4 abundance...
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作者：Poon, Michael M.; Lorrain, Kym I.; Stebbins, Karin J.; Edu, Geraldine C.; Broadhead, Alexander R.; Lorenzana, Ariana J.; Roppe, Jeffrey R.; Baccei, Jill M.; Baccei, Christopher S.; Chen, Austin C.; Green, Ari J.; Lorrain, Daniel S.; Chan, Jonah R.
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作者：Ju, Zhengyu; Zheng, Tianrui; Zhang, Bowen; Dolocan, Andrei; Marschilok, Amy C.; Takeuchi, Esther S.; Takeuchi, Kenneth J.; Yu, Guihua
作者单位：University of Texas System; University of Texas Austin; University of Texas System; University of Texas Austin; University of Texas System; University of Texas Austin; State University of New York (SUNY) System; Stony Brook University; State University of New York (SUNY) System; Stony Brook University; United States Department of Energy (DOE); Brookhaven National Laboratory; State University of New York (SUNY) System; Stony Brook University
摘要：Lithium (Li) metal has been recognized as a promising anode to advance the energy density of current Li-based batteries. However, the growth of the solid-electrolyte interphase (SEI) layer and dendritic Li microstructure pose significant challenges for the long-term operation of Li metal batteries (LMBs). Herein, we propose the utilization of a suspension electrolyte with dispersed magnetically responsive nanosheets whose orientation can be manipulated by an external magnetic field during cell...
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作者：Waller, Alexis A.; Ribardo, Deborah A.; Hendrixson, David R.
作者单位：University of Texas System; University of Texas Southwestern Medical Center
摘要：Bacteria power rotation of an extracellular flagellar filament for swimming motility. Thousands of flagellin subunits compose the flagellar filament, which extends several microns from the bacterial surface. It is unclear whether bacteria actively control filament length. Many polarly flagellated bacteria produce shorter flagellar filaments than peritrichous bacteria, and FlaG has been reported to limit flagellar filament length in polar flagellates. However, a mechanism for how FlaG may funct...
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作者：Zhao, Zijie; Yang, Xiaoyu; Dorn, Stephen; Miao, Jiacheng; Barcellos, Silvia H.; Fletcher, Jason M.; Lu, Qiongshi
作者单位：University of Wisconsin System; University of Wisconsin Madison; University of Southern California; University of Southern California; University of Wisconsin System; University of Wisconsin Madison; University of Wisconsin System; University of Wisconsin Madison
摘要：Epidemiologic associations estimated from observational data are often confounded by genetics due to pervasive pleiotropy among complex traits. Many studies either neglect genetic confounding altogether or rely on adjusting for polygenic scores (PGS) in regression analysis. In this study, we unveil that the commonly employed PGS approach is inadequate for removing genetic confounding due to measurement error and model misspecification. To tackle this challenge, we introduce PENGUIN, a principl...
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作者：Chen, Jiaxing; Hnath, Brianna; Sha, Congzhou M.; Beidler, Lynne; Schell, Todd D.; Dokholyan, Nikolay V.
作者单位：Pennsylvania Commonwealth System of Higher Education (PCSHE); Pennsylvania State University; Penn State Health; Pennsylvania Commonwealth System of Higher Education (PCSHE); Pennsylvania State University; Pennsylvania State University - University Park; Pennsylvania Commonwealth System of Higher Education (PCSHE); Pennsylvania State University; Pennsylvania State University - University Park; Pennsylvania Commonwealth System of Higher Education (PCSHE); Pennsylvania State University; Penn State Health; Pennsylvania Commonwealth System of Higher Education (PCSHE); Pennsylvania State University; Penn State Health; Pennsylvania Commonwealth System of Higher Education (PCSHE); Pennsylvania State University; Pennsylvania State University - University Park
摘要：Chimeric antigen receptor T cell therapies have achieved great success in eradicating some liquid tumors, whereas the preclinical results in treating solid tumors have proven less decisive. One of the principal challenges in solid tumor treatment is the physical barrier composed of a dense extracellular matrix, which prevents immune cells from penetrating the tissue to attack intratumoral cancer cells. Here, we improve immune cell infiltration into solid tumors by manipulating septin-7 functio...
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作者：Cieslinskia, Pawel; Knips, Lukas; Kowalczyk, Mateusz; Laskowski, Wieshw; Paterek, Tomasz; Vertesi, Tamas; Weinfurter, Harald
作者单位：Fahrenheit Universities; University of Gdansk; Max Planck Society; University of Munich; Xiamen University Malaysia Campus; HUN-REN; HUN-REN Institute for Nuclear Research
摘要：Quantum mechanics imposes limits on the statistics of certain observables. Perhaps the most famous example is the uncertainty principle. Similar trade-offs also exist for the simultaneous violation of multiple Bell inequalities. In the simplest case of three observers, it has been shown that if two observers violate a Bell inequality, then none of them can violate any Bell inequality with the third observer, a property called monogamy of Bell violations. Forms of Bell monogamy have been linked...
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作者：Faizi, Hammad A.; Granek, Rony; Vlahovska, Petia M.
作者单位：Northwestern University; Ben-Gurion University of the Negev; Ben-Gurion University of the Negev; Northwestern University; Northwestern University; University of Chicago
摘要：The biological function of membranes is closely related to their softness, which is often studied through the membranes' thermally driven fluctuations. Typically, the analysis assumes that the relaxation rate of a pure bending deformation is determined by the competition between membrane bending rigidity and viscous dissipation in the surrounding medium. Here, we reexamine this assumption and demonstrate that viscous flows within the membrane dominate the dynamics of bending fluctuations of no...
