45期

最新文章

• A CRISPR-Cas9 knockout screening identifies IRF2 as a key driver of OAS3/RNase L- mediated RNA decay during viral infection AI翻译
  作者：Oh, Sunwoo; Santiago, Gisselle; Manjunath, Lavanya; Li, Junyi; Bouin, Alexis; Semler, Bert L.; Buisson, Remi
  作者单位：University of California System; University of California Irvine; University of California System; University of California Irvine
  摘要：OAS-RNase L is a double- stranded RNA- induced antiviral pathway triggered in response to diverse viral infections. Upon activation, OAS-RNase L suppresses virus replication by promoting the decay of host and viral RNAs and inducing translational shutdown. However, whether OASs and RNase L are the only factors involved in this pathway remains unclear. Here, we develop CRISPR- Translate, a FACS-based genome-wide CRISPR-Cas9 knockout screening method that uses translation levels as a readout and...
• Reverse development in the ctenophore Mnemiopsis leidyi AI翻译
  作者：Soto-Angel, Joan J.; Burkhardt, Pawel
  作者单位：University of Bergen; University of Bergen
  摘要：Reverse development, or the ability to rejuvenate by morphological reorganization into the preceding life cycle stage is thought to be restricted to a few species within Cnidaria. To date, Turritopsis dohrnii is the only known species capable of undergoing reverse development after the onset of sexual reproduction. Here, we demonstrate that the ctenophore Mnemiopsis leidyi is capable of reversal from mature lobate to early cydippid when fed following a period of stress. Our findings illuminate...
• Is stochastic thermodynamics the key to understanding the energy costs of computation? AI翻译
  作者：Wolpert, David H.; Korbel, Jan; Lynn, Christopher W.; Tasnim, Farita; Grochow, Joshua A.; Kardes, Gulce; Aimone, James B.; Balasubramanian, Vijay; De Giuli, Eric; Doty, David; Freitas, Nahuel; Marsili, Matteo; Ouldridge, Thomas E.; Richa, Andrea W.; Riechers, Paul; Roldan, Edgar; Rubenstein, Brenda; Toroczkai, Zoltan; Paradiso, Joseph
  作者单位：The Santa Fe Institute; Arizona State University; Arizona State University-Tempe; Abdus Salam International Centre for Theoretical Physics (ICTP); Yeshiva University; Medical University of Vienna; Princeton University; City University of New York (CUNY) System; Yale University; City University of New York (CUNY) System; University of Colorado System; University of Colorado Boulder; United States Department of Energy (DOE); Sandia National Laboratories; University of Pennsylvania; University of Oxford; Toronto Metropolitan University; University of California System; University of California Davis; University of Buenos Aires; Imperial College London; Imperial College London; Nanyang Technological University; Brown University; University of Notre Dame; Massachusetts Institute of Technology (MIT)
  摘要：The relationship between the thermodynamic and computational properties of physical systems has been a major theoretical interest since at least the 19th century. It has also become of increasing practical importance over the last half-century as the energetic cost of digital devices has exploded. Importantly, real-world computers obey multiple physical constraints on how they work, which affects their thermodynamic properties. Moreover, many of these constraints apply to both naturally occurr...
• Facilitating and restraining virus infection using cell- attachable soluble viral receptors AI翻译
  作者：Zhang, Heng; Wang, Zhengli; Nguyen, Huong T. T.; Pontelli, Marjorie Cornejo; Qi, Wanrong; Rao, Liem; Liu, Zhuoming; Whelan, Sean P. J.; Zhu, Jieqing
  作者单位：Washington University (WUSTL); Medical College of Wisconsin
  摘要：SARS-CoV-2 uses the receptor binding domain (RBD) of its spike protein to recognize and infect host cells by binding to the cell surface receptor angiotensin converting enzyme 2 (ACE2). The ACE2 receptor is composed of peptidase domain (PD), collectrin-like domain, transmembrane domain, and short cytoplasmic domain, and may exist as a dimer on cell surface. The RBD binding site is located atop of the ACE2 PD, but the involvement of other domains in virus infection is uncertain. We found that t...
• Reducing posttreatment relapse in cleft lip palatal expansion using an injectable estrogen-nanodiamond hydrogel ( vol 114, pg E7218, 2017) AI翻译
  作者：Hong, Christine; Song, Dayoung; Lee, Dong-Keun; Lin, Lawrence; Pan, Hsin Chuan; Lee, Deborah; Deng, Peng; Liu, Zhenqing; Hadaya, Danny; Lee, Hye-Lim; Mohammad, Abdulaziz; Zhang, Xinli; Lee, Min; Wang, Cun-Yu; Ho, Dean
• Genome-wide profiling of soybean WRINKLED1 transcription factor binding sites provides insight into seed storage lipid biosynthesis AI翻译
  作者：Jo, Leonardo; Pelletier, Julie M.; Goldberg, Robert B.; Harada, John J.
  作者单位：University of California System; University of California Davis; University of California System; University of California Los Angeles; Utrecht University
  摘要：Understanding the regulatory mechanisms controlling storage lipid accumulation will inform strategies to enhance seed oil quality and quantity in crop plants. The WRINKLED1 transcription factor (WRI1 TF) is a central regulator of lipid biosynthesis. We characterized the genome-wide binding profile of soybean (Gm)WRI1 and show that the TF directly regulates genes encoding numerous enzymes and proteins in the fatty acid and triacylglycerol biosynthetic pathways. GmWRI1 binds primarily to regions...
• Atomic view of photosynthetic metabolite permeability pathways and confinement in synthetic carboxysome shells AI翻译
  作者：Sarkar, Daipayan; Maffeo, Christopher; Sutter, Markus; Aksimentiev, Aleksei; Kerfeld, Cheryl A.; Vermaas, Josh V.
  作者单位：Michigan State University; United States Department of Energy (DOE); University of Illinois System; University of Illinois Urbana-Champaign; United States Department of Energy (DOE); Lawrence Berkeley National Laboratory; United States Department of Energy (DOE); Lawrence Berkeley National Laboratory; University of Illinois System; University of Illinois Urbana-Champaign; Michigan State University
  摘要：Carboxysomes are protein microcompartments found in cyanobacteria, whose shell encapsulates rubisco at the heart of carbon fixation in the Calvin cycle. Carboxysomes are thought to locally concentrate CO2 in the shell interior to improve rubisco efficiency through selective metabolite permeability, creating a concentrated catalytic center. However, permeability coefficients have not previously been determined for these gases, or for Calvin-cycle intermediates such as bicarbonate (HCO-3 ), 3-ph...
• Carbon-centric dynamics of Earth's marine phytoplankton AI翻译
  作者：Stoer, Adam C.; Fennel, Katja
  作者单位：Dalhousie University
  摘要：Marine phytoplankton are fundamental to Earth's ecology and biogeochemistry. Our understanding of the large-scale dynamics of phytoplankton biomass has greatly benefited from, and is largely based on, satellite ocean color observations from which chlorophyll-a (Chla), a commonly used proxy for carbon biomass, can be estimated. However, ocean color satellites only measure a small portion of the surface ocean, meaning that subsurface phytoplankton biomass is not directly monitored. Chla is also ...
• Higher oxygen content and transport characterize high- altitude ethnic Tibetan women with the highest lifetime reproductive success AI翻译
  作者：Ye, Shenghao; Sun, Jiayang; Craig, Sienna R.; Di Rienzo, Anna; Witonsky, David; Yu, James J.; Moya, Esteban A.; Simonson, Tatum S.; Powell, Frank L.; Basnyat, Buddha; Strohl, Kingman P.; Hoit, Brian D.; Beall, Cynthia M.
  作者单位：George Mason University; Dartmouth College; University of Chicago; University of California System; University of California San Diego; University of Oxford; University System of Ohio; Case Western Reserve University; University System of Ohio; Case Western Reserve University; University Hospitals of Cleveland; University System of Ohio; Case Western Reserve University; University Hospitals of Cleveland; University System of Ohio; Case Western Reserve University
  摘要：We chose the natural laboratory provided by highaltitude native ethnic Tibetan women who had completed childbearing to examine the hypothesis that multiple oxygen delivery traits were associated with lifetime reproductive success and had genomic associations. Four hundred seventeen (417) women aged 46 to 86 y residing at >= 3,500 m in Upper Mustang, Nepal, provided information on reproductive histories, sociocultural factors, physiological measurements, and DNA samples for this observational c...
• Measurement of α- synuclein as protein cargo in plasma extracellular vesicles AI翻译
  作者：Gilboa, Tal; Ter-Ovanesyan, Dmitry; Wang, Shih-Chin; Whiteman, Sara; Kannarkat, George T.; Church, George M.; Chen-Plotkin, Alice S.; Walt, David R.
  作者单位：Harvard University; Harvard University; Harvard University Medical Affiliates; Brigham & Women's Hospital; Harvard University; Harvard Medical School; University of Pennsylvania
  摘要：Extracellular vesicles (EVs) are released by all cells and hold great promise as a class of biomarkers. This promise has led to increased interest in measuring EV proteins from both total EVs as well as brain- derived EVs in plasma. However, measuring cargo proteins in EVs has been challenging because EVs are present at low levels, and EV isolation methods are imperfect at separating EVs from free proteins. Thus, knowing whether a protein measured after EV isolation is truly inside EVs is diff...