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作者：Carey, John
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作者：Constable, Sandii; Ott, Carolyn M.; Lemire, Andrew L.; White, Kevin; Xun, Yu; Lim, Amin; Lippincott-Schwartz, Jennifer; Mukhopadhyay, Saikat
作者单位：University of Texas System; University of Texas Southwestern Medical Center; Howard Hughes Medical Institute
摘要：Brain neurons utilize the primary cilium as a privileged compartment to detect and respond to extracellular ligands such as Sonic hedgehog (SHH). However, cilia in cerebellar granule cell (GC) neurons disassemble during differentiation through ultrastructurally unique intermediates, a process we refer to as cilia deconstruction. In addition, mature neurons do not reciliate despite having docked centrioles. Here, we identify molecular changes that accompany cilia deconstruction and centriole do...
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作者：Furtado, Jessica; Geraldo, Luiz Henrique; Leser, Felipe Saceanu; Bartkowiak, Bartlomiej; Poulet, Mathilde; Park, Hyojin; Robinson, Mark; Pibouin-Fragner, Laurence; Eichmann, Anne; Boye, Kevin
作者单位：Yale University; Yale University; Institut National de la Sante et de la Recherche Medicale (Inserm); Universite Paris Cite; Yale University; Yale University
摘要：The integrity of the blood-retina barrier (BRB) is crucial for phototransduction and vision, by tightly restricting transport of molecules between the blood and surrounding neuronal cells. Breakdown of the BRB leads to the development of retinal diseases. Here, we show that Netrin- 1/Unc5b and Norrin/Lrp5 signaling establish a zonated endothelial cell gene expression program that controls BRB integrity. Using single- cell RNA sequencing (scRNA- seq) of postnatal BRB- competent mouse retina end...
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作者：Glynatsi, Nikoleta E.; Akin, Ethan; Nowak, Martin A.; Hilbe, Christian
作者单位：Max Planck Society; City University of New York (CUNY) System; City College of New York (CUNY); Harvard University; Harvard University
摘要：Direct reciprocity is a wide-spread mechanism for the evolution of cooperation. repeated interactions, players can condition their behavior on previous outcomes. well-known approach is given by reactive strategies, which respond to the coplayer's previous move. Here, we extend reactive strategies to longer memories. A reactive n strategy takes into account the sequence of the last n moves of the coplayer. reactive-n counting strategy responds to how often the coplayer cooperated during the las...
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作者：Yang, Haotian; Wang, Lihang; Li, Yuqiang; Zhuo, Zengqing; Wu, Tianhao; Liu, Jie; Xu, Ligang; Du, Haozhe; Liu, Shiqi; Wu, Lingqiao; Zhao, Shu; Tang, Mingxue; Yang, Wanli; Yu, Haijun
作者单位：Beijing University of Technology; Beijing University of Technology; United States Department of Energy (DOE); Lawrence Berkeley National Laboratory; University of Science & Technology Beijing
摘要：Lithium- rich layered oxides (LLOs) hold the promise for high- energy battery cathodes. However, its application has been hindered by voltage decay associated with irreversible reactions at high voltages despite decades of intensive efforts. Here, we first theoretically studied the molecular orbitals of Mn-based Li- rich configurations. We found that the it- bond ring formed within the LiMn6 structure could participate in stable redox reactions as one unit, but Co could disrupt its symmetry. W...
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作者：Dai, Lei; Wu, Zuowei; Sahin, Orhan; Zhao, Shaohua; Yu, Edward W.; Zhang, Qijing
作者单位：Iowa State University; Iowa State University; US Food & Drug Administration (FDA); University System of Ohio; Case Western Reserve University
摘要：The resistance- nodulation-cell division (RND) superfamily of multidrug efflux systems are important players in mediating antibiotic resistance in gram- negative pathogens. Campylobacter jejuni, a major enteric pathogen, utilizes an RND-type transporter system, CmeABC, as the primary mechanism for extrusion of various antibiotics. Recently, a functionally potent variant of CmeABC (named RE- CmeABC) emerged in clinical Campylobacter isolates, conferring enhanced resistance to multiple antibioti...
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作者：Jo, Wendy K.; Cassiano, Murilo Henrique Anzolini; de Oliveira-Filho, Edmilson Ferreira; Bruenink, Sebastian; Yansanjav, Adiya; Yihune, Mesele; Koshkina, Alyona I.; Lukashev, Alexander N.; Lavrenchenko, Leonid A.; Lebedev, Vladimir S.; Olayemi, Ayodeji; Bangura, Umaru; Salas- Rojask, Monica; Aguilar-Setienk, Alvaro; Fichet- Calvetj, Elisabeth; Drexlera, Jan Felix
作者单位：Free University of Berlin; Humboldt University of Berlin; Charite Universitatsmedizin Berlin; Free University of Berlin; Humboldt University of Berlin; Mongolian Academy of Sciences; Addis Ababa University; Association for the Conservation of Biodiversity of Kazakhstan; Sechenov First Moscow State Medical University; Russian Academy of Sciences; Saratov Scientific Center of the Russian Academy of Sciences; Severtsov Institute of Ecology & Evolution; Lomonosov Moscow State University; Obafemi Awolowo University; Njala University; Leibniz Association; Bernhard Nocht Institut fur Tropenmedizin
摘要：Hepatitis E virus (HEY; family Hepeviridae) infections cause >40,000 human deaths annually. Zoonotic infections predominantly originate from ungulates and occasionally from rats, highlighting the zoonotic potential of rodent- associated hepeviruses. We conducted host genomic data mining and uncovered two genetically divergent rodent- associated hepeviruses, and two bat- associated hepeviruses genetically related to known bat- associated strains. We thus analyzed 2,565 liver specimens from 108 ...
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作者：Kammer, Kai-Michael; Eisgruber, Terese; Heid, Peter; Pellarin, Riccardo; Stengel, Florian
作者单位：University of Konstanz; University of Konstanz; Centre National de la Recherche Scientifique (CNRS); CNRS - National Institute for Biology (INSB); Pasteur Network; Universite Paris Cite; Institut Pasteur Paris; CHU Lyon; Centre National de la Recherche Scientifique (CNRS); Ecole Normale Superieure de Lyon (ENS de LYON); Universite Claude Bernard Lyon 1; Universite Jean Monnet; Institut National de la Sante et de la Recherche Medicale (Inserm)
摘要：Proteins play a central role in most biological processes within the cell, and deciphering how they interact is key to understand their function. Cross- linking coupled with mass spectrometry is an essential tool for elucidating protein-protein interactions (PPIs). Despite its importance, we still know surprisingly little about the principles that underlie the process of chemical cross- link formation itself and how it is influenced by different physicochemical factors. To understand the molec...
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作者：Woodie, Lauren N.; Alberto, Ahren J.; Krusen, Brianna M.; Melink, Lily C.; Lazar, Mitchell A.
作者单位：University of Pennsylvania
摘要：Nearly every cell of the body contains a circadian clock mechanism that is synchronized with the light- entrained clock in the suprachiasmatic nucleus (SCN). Desynchrony between the SCN and the external environment leads to metabolic dysfunction in shift workers. Similarly, mice with markedly shortened endogenous period due to the deletion of circadian REV- ERB alpha/beta nuclear receptors in the SCN (SCN DKO) exhibit increased sensitivity to diet- induced obesity (DIO) on a 24 h light:dark cy...
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作者：Ding, Erika A.; Yokokura, Takashi J.; Wang, Rui; Kumar, Sanjay
作者单位：University of California System; University of California Berkeley; United States Department of Energy (DOE); Lawrence Berkeley National Laboratory; University of California System; University of California Berkeley; University of California System; University of California San Francisco
摘要：Neurofilaments (NFs) are multisubunit, bottlebrush- shaped intermediate filaments abundant in the axonal cytoskeleton. Each NF subunit contains a long intrinsically disordered tail domain, which protrudes from the NF core to form a brush surrounding each NF. Precisely how the tails' variable charge patterns and repetitive phosphorylation sites mediate their conformation within the brush remains an open question in axonal biology. We address this problem by grafting recombinant NF tail protein ...
