2024

最新文章

• Selective 8-oxo- rG stalling occurs in the catalytic core of polynucleotide phosphorylase (PNPase) during degradation AI翻译
  作者：Miller, Lucas G.; Kim, Wantae; Schowe, Shawn; Taylor, Kathleen; Han, Runhua; Jain, Vashita; Park, Raeyeon; Sherman, Mark; Fang, Janssen; Ramirez, Haydee; Ellington, Andrew; Tamamis, Phanourios; Resendiz, Marino J. E.; Zhang, Y. Jessie; Contreras, Lydia
  作者单位：University of Texas System; University of Texas Austin; University of Colorado System; University of Colorado Anschutz Medical Campus; Children's Hospital Colorado; University of Colorado Denver; Texas A&M University System; Texas A&M University College Station; University of Texas System; University of Texas Austin; Texas A&M University System; Texas A&M University College Station
  摘要：RNA oxidation, predominantly through the accumulation of 8-oxo-7,8-dihydroguanosine (8-oxo-rG), represents an important biomarker for cellular oxidative stress. Polynucleotide phosphorylase (PNPase) is a 3 '-5 ' exoribonuclease that has been shown to preferentially recognize 8-oxo-rG-containing RNA and protect Escherichia coli cells from oxidative stress. However, the impact of 8-oxo-rG on PNPase-mediated RNA degradation has not been studied. Here, we show that the presence of 8-oxo-rG in RNA ...
• Architecture of the Toxoplasma gondii apical polar ring and its role in gliding motility and invasion AI翻译
  作者：Ren, Bingjian; Haase, Romuald; Patray, Sharon; Nguyen, Quynh; Maco, Bohumil; Pacheco, Nicolas Dos Santos; Chang, Yi-Wei; Soldati-Favre, Dominique
  作者单位：University of Geneva; University of Pennsylvania; University of Pennsylvania; University of Cambridge
  摘要：In Toxoplasma gondii, the conoid comprises a cone with spiraling tubulin fibers, preconoidal rings, and intraconoidal microtubules. This dynamic organelle undergoes extension and retraction through the apical polar ring (APR) during egress, gliding, and invasion. The forces involved in conoid extrusion are beginning to be understood, and its role in directing F- actin flux to the pellicular space, thereby controlling parasite motility, has been proposed. However, the contribution of the APR an...
• How to survive enormous amounts of alcohol AI翻译
  作者：Spanagel, Rainer
  作者单位：Central Institute of Mental Health; Ruprecht Karls University Heidelberg
• Combatting melioidosis with chemical synthetic lethality AI翻译
  作者：Zhang, Yifan; Mcwhorter, Kirklin L.; Rosen, Paul C.; Klaus, Jennifer R.; Gallant, Etienne; Lopez, Cindy Y. Amaya; Jhunjhunwala, Riddhi; Chandler, Josephine R.; Davis, Katherine M.; Seyedsayamdost, Mohammad R.
  作者单位：Princeton University; Emory University; Princeton University; University of Kansas
  摘要：Burkholderia thailandensis has emerged as a nonpathogenic surrogate for Burkholderia pseudomallei, the causative agent of melioidosis, and an important Gram- negative model bacterium for studying the biosynthesis and regulation of secondary metabolism. We recently reported that subinhibitory concentrations of trimethoprim induce vast changes in both the primary and secondary metabolome of B. thailandensis. In the current work, we show that the folate biosynthetic enzyme FolE2 is permissive und...
• Perturbation- specific transcriptional mapping for unbiased target elucidation of antibiotics AI翻译
  作者：Romano, Keith P.; Bagnall, Josephine; Warrier, Thulasi; Sullivan, Jaryd; Ferrara, Kristina; Orzechowski, Marek; Nguyen, Phuong H.; Raines, Kyra; Livny, Jonathan; Shoresh, Noam; Hung, Deborah T.
  作者单位：Harvard University; Massachusetts Institute of Technology (MIT); Broad Institute; Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; Harvard University; Harvard University Medical Affiliates; Brigham & Women's Hospital; Harvard University; Harvard Medical School
  摘要：The rising prevalence of antibiotic resistance threatens human health. While more sophisticated strategies for antibiotic discovery are being developed, target elucidation of new chemical entities remains challenging. In the postgenomic era, expression profiling can play an important role in mechanism- of- action (MOA) prediction by reporting on the cellular response to perturbation. However, the broad application of transcriptomics has yet to fulfill its promise of transforming target elucida...
• Gut microbes participate in host polyamine metabolism AI翻译
  作者：Yu, Leilei; Zhang, Chengcheng; Zhai, Qixiao
  作者单位：Jiangnan University; Jiangnan University; Jiangnan University
• Complement activation by IgG subclasses is governed by their ability to oligomerize upon antigen binding AI翻译
  作者：Frischauf, Nikolaus; Borg, Ellen G. F.; Labrijn, Aran F.; Beurskens, Frank J.; Preiner, Johannes
  作者单位：Genmab
  摘要：Complement activation through antibody-antigen complexes is crucial in various pathophysiological processes and utilized in immunotherapies to eliminate infectious agents, regulatory immune cells, or cancer cells. The tertiary structures of the four IgG antibody subclasses are largely comparable, with the most prominent difference being the hinge regions connecting the Fab and Fc domains, providing them with unique structural flexibility. Complement recruitment and activation depend strongly o...
• Toll-like receptor 3 (TLR3) activation induces microRNA-dependent reexpression of functional RARβ and tumor regression (vol 110, pg 9812, 2013) AI翻译
  作者：Galli, Roberta; Paone, Alessio; Fabbri, Muller; Zanesi, Nicola; Calore, Federica; Cascione, Luciano; Acunzo, Mario; Stoppacciaro, Antonella; Tubaro, Andrea; Lovat, Francesca; Gasparini, Pierluigi; Fadda, Paolo; Alder, Hansjuerg; Volinia, Stefano; Filippini, Antonio; Ziparo, Elio; Riccioli, Anna; Croce, Carlo M.
• Abortive infection of bat fibroblasts with SARS- CoV-2 AI翻译
  作者：Bisht, Punam; Gallagher, Michael D.; Barrasa, M. Inmaculada; Boucau, Julie; Harding, Alfred; Dejosez, Marion; Parejo, Carlos Godoy -; Bisher, Margaret E.; de Nola, Giovanni; Lytton-Jean, Abigail K. R.; Gehrke, Lee; Zwaka, Thomas P.; Jaenisch, Rudolf
  作者单位：Massachusetts Institute of Technology (MIT); Whitehead Institute; Harvard University; Massachusetts Institute of Technology (MIT); Ragon Institute; Massachusetts Institute of Technology (MIT); Harvard University; Harvard Medical School; Icahn School of Medicine at Mount Sinai; Icahn School of Medicine at Mount Sinai; Massachusetts Institute of Technology (MIT); Massachusetts Institute of Technology (MIT)
  摘要：Bats are tolerant to highly pathogenic viruses such as Marburg, Ebola, and Nipah, suggesting the presence of a unique immune tolerance toward viral infection. Here, we compared severe acute respiratory syndrome coronavirus 2 (SARS- CoV-2) infection of human and bat ( Rhinolophus ferrumequinum) pluripotent cells and fibroblasts. Since bat cells do not express an angiotensin- converting enzyme 2 (ACE2) receptor that allows virus infection, we transduced the human ACE2 (hA) receptor into the cell...
• Nuclear p62 condensates stabilize the promyelocytic leukemia nuclear bodies by sequestering their ubiquitin ligase RNF4 AI翻译
  作者：Fu, Afu; Luo, Zhiwen; Ziv, Tamar; Bi, Xinyu; Lulu-Shimron, Chen; Cohen-Kaplan, Victoria; Ciechanover, Aaron
  作者单位：Technion Israel Institute of Technology; Rappaport Faculty of Medicine; Technion Israel Institute of Technology; Chinese Academy of Medical Sciences - Peking Union Medical College; Cancer Institute & Hospital - CAMS; Peking Union Medical College; Technion Israel Institute of Technology; Technion Israel Institute of Technology
  摘要：Liquid-liquid phase separation has emerged as a crucial mechanism driving the formation of membraneless biomolecular condensates, which play important roles in numerous cellular processes. These condensates, found both in the nucleus and cytoplasm, are formed through multivalent, low- affinity interactions between various molecules. P62- containing condensates serve, among other functions, as proteolytic hubs for the ubiquitin-proteasome system. In this study, we investigated the dynamic inter...