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作者：Xiao, Qiang; Cruz, Gabrielle; Botham, Rachel; Fox, Susan G.; Yu, Anan; Allen, Seth; Morimoto, Richard I.; Kelly, Jeffery W.
作者单位：Scripps Research Institute; Scripps Research Institute; State University of New York (SUNY) System; SUNY Fredonia; Northwestern University
摘要：Macroautophagy is a conserved cellular degradation pathway that, upon upregulation, confers resilience toward various stress conditions, including protection against proteotoxicity associated with neurodegenerative diseases, leading to cell survival. Monitoring autophagy regulation in living cells is important to understand its role in physiology and pathology, which remains challenging. Here, we report that when HaloTag is expressed within a cell of interest and reacts with tetramethylrhodami...
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作者：Guarnieri, Joseph W.; Lie, Timothy; Albrecht, Yentli E. Soto; Hewin, Peter; Jurado, Kellie A.; Widjaja, Gabrielle A.; Zhu, Yi; McManus, Meagan J.; Kilbaugh, Todd J.; Keith, Kelsey; Potluri, Prasanth; Taylor, Deanne; Angelin, Alessia; Murdock, Deborah G.; Wallace, Douglas C.
作者单位：University of Pennsylvania; Pennsylvania Medicine; Childrens Hospital of Philadelphia; University of Pennsylvania; University of Pennsylvania; University of Pennsylvania; Pennsylvania Medicine; Childrens Hospital of Philadelphia; University of Pennsylvania; Pennsylvania Medicine; Childrens Hospital of Philadelphia; University of Pennsylvania
摘要：Severe Acute Respiratory Syndrome Coronavirus 2 (SARS- CoV-2) infection inhibits mitochondrial oxidative phosphorylation (OXPHOS) and elevates mitochondrial reactive oxygen species (ROS, mROS) which activates hypoxia- inducible factor- 1alpha (HIF-1 alpha), shifting metabolism toward glycolysis to drive viral biogenesis but also causing the release of mitochondrial DNA (mtDNA) and activation of innate immunity. To determine whether mitochondrially targeted antioxidants could mitigate these vir...
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作者：Lequieu, Joshua
作者单位：Drexel University
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作者：Sandercock, Alexander M.; Westbrook, Jared W.; Zhang, Qian; Holliday, Jason A.
作者单位：Virginia Polytechnic Institute & State University; Virginia Polytechnic Institute & State University; Cornell University
摘要：American chestnut ( Castanea dentata) is a deciduous tree species of eastern North America that was decimated by the introduction of the chestnut blight fungus ( Cryphonectria parasitica) in the early 20th century. Although millions of American chestnuts survive as root collar sprouts, these trees rarely reproduce. Thus, the species is considered functionally extinct. American chestnuts with improved blight resistance have been developed through interspecific hybridization followed by conspeci...
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作者：Zin, Isaac; China, Arnab; Khan, Krishnendu; Nag, Jeetendra K.; Vasu, Kommireddy; Deshpande, Gauravi M.; Ghosh, Prabar K.; Khan, Debjit; Ramachandiran, Iyappan; Ganguly, Shinjini; Tamagno, Ilaria; Willard, Belinda; Gogonea, Valentin; Fox, Paul L.
作者单位：Cleveland Clinic Foundation; University System of Ohio; Cleveland State University; Cleveland Clinic Foundation; Gandhi Institute of Technology & Management (GITAM); Cleveland Clinic Foundation; Cleveland Clinic Foundation
摘要：Glutamyl- prolyl-tRNA synthetase (EPRS1) is a bifunctional aminoacyl-tRNA- synthetase (aaRS) essential for decoding the genetic code. EPRS1 resides, with seven other aaRSs and three noncatalytic proteins, in the cytoplasmic multi-tRNA synthetase complex (MSC). Multiple MSC- resident aaRSs, including EPRS1, exhibit stimulus- dependent release from the MSC to perform noncanonical activities distinct from their primary function in protein synthesis. Here, we show EPRS1 is present in both cytoplas...
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作者：Driver, Justin G.; Bernard, Ellina; Patrizio, Piera; Fennell, Paul S.; Scrivener, Karen; Myers, Rupert J.
作者单位：Imperial College London; Imperial College London; Swiss Federal Institutes of Technology Domain; Swiss Federal Laboratories for Materials Science & Technology (EMPA); Imperial College London; Swiss Federal Institutes of Technology Domain; Ecole Polytechnique Federale de Lausanne
摘要：CO2 2 mineralization products are often heralded as having outstanding potentials to reduce CO2- eq. 2- eq. emissions. However, these claims are generally undermined by incomplete consideration of the life cycle climate change impacts, material properties, supply and demand constraints, and economic viability of CO2 2 mineralization products. We investigate these factors in detail for ten concrete-- related CO2 2 mineralization products to quantify their individual and global CO2- eq. 2- eq. e...
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作者：Kunkle, Dillon E.; Cai, Yujuan; Eichman, Brandt F.; Skaar, Eric P.
作者单位：Vanderbilt University; Vanderbilt University; Vanderbilt University; Vanderbilt University
摘要：Maintenance of DNA integrity is essential to all forms of life. DNA damage generated by reaction with genotoxic chemicals results in deleterious mutations, genome instability, and cell death. Pathogenic bacteria encounter several genotoxic agents during infection. In keeping with this, the loss of DNA repair networks results in virulence attenuation in several bacterial species. Interstrand DNA crosslinks (ICLs) are a type of DNA lesion formed by covalent linkage of opposing DNA strands and ar...
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作者：Guo, Jiashu; Zhang, Peipei; Wu, Nan; Liu, Wenwen; Liu, Yan; Jin, Huaibing; Francis, Frederic; Wang, Xifeng
作者单位：Chinese Academy of Agricultural Sciences; Institute of Plant Protection, CAAS; University of Liege; Langfang Normal University; Chinese Academy of Agricultural Sciences
摘要：Although most known viruses infecting fungi pathogenic to higher eukaryotes are asymptomatic or reduce the virulence of their host fungi, those that confer hypervirulence to entomopathogenic fungus still need to be explored. Here, we identified and studied a novel mycovirus in Metarhizium flavoviride, isolated from small brown planthopper (Laodelphax striatellus). Based on molecular analysis, we tentatively designated the mycovirus as Metarhizium flavoviride partitivirus 1 (MfPV1), a species i...
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作者：Nam, Chehyun; Li, Li-Yan; Yang, Qian; Ziman, Benjamin; Zhao, Hua; Hu, Boyan; Collet, Casey; Jing, Pei; Lei, Qifang; Xu, Li-Yan; Li, En- Min; Koeffler, H. Phillip; Sinha, Uttam K.; Lin, De- Chen
作者单位：University of Southern California; Shantou University; Cedars Sinai Medical Center; Shenzhen University; University of Southern California
摘要：Upper aerodigestive squamous cell carcinoma (UASCC) is a common and aggressive malignancy with few effective therapeutic options. Here, we investigate amino acid metabolism in this cancer, surprisingly noting that UASCC exhibits the highest methionine level across all human cancers, driven by its transporter LAT1. We show that LAT1 is also expressed at the highest level in UASCC, transcriptionally activated by UASCC - specific promoter and enhancers, which are directly coregulated by SCC maste...
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作者：Chen, Pu; Chen, Yukun; Xia, Ningbo; Fan, Bolin; Niu, Zhipeng; He, Zhengming; Wang, Xu; Yuan, Jing; Gupta, Nishith; Goldberg, Daniel; Shen, Bang
作者单位：Huazhong Agricultural University; Hubei Hongshan Laboratory; Xiamen University; Birla Institute of Technology & Science Pilani (BITS Pilani); Humboldt University of Berlin; Huazhong Agricultural University; Chinese Academy of Agricultural Sciences; Agriculture Genomes Institute at Shenzhen, CAAS
摘要：Pyruvate lies at a pivotal node of carbon metabolism in eukaryotes. It is involved in diverse metabolic pathways in multiple organelles, and its interorganelle shuttling is crucial for cell fitness. Many apicomplexan parasites harbor a unique organelle called the apicoplast that houses metabolic pathways like fatty acid and isoprenoid precursor biosyntheses, requiring pyruvate as a substrate. However, how pyruvate is supplied in the apicoplast remains enigmatic. Here, deploying the zoonotic pa...
