2024

最新文章

• Spatiotemporal transcriptomic map of glial cell response in a mouse model of acute brain ischemia AI翻译
  作者：Zucha, Daniel; Abaffy, Pavel; Kirdajova, Denisa; Jirak, Daniel; Kubista, Mikael; Anderova, Miroslava; Valihrach, Lukas
  作者单位：Czech Academy of Sciences; Institute of Biotechnology of the Czech Academy of Sciences; University of Chemistry & Technology, Prague; Czech Academy of Sciences; Institute of Experimental Medicine of the Czech Academy of Sciences; Institute for Clinical & Experimental Medicine (IKEM); Technical University of Liberec
  摘要：The role of nonneuronal cells in the resolution of cerebral ischemia remains to be fully understood. To decode key molecular and cellular processes that occur after ischemia, we performed spatial and single- cell transcriptomic profiling of the male mouse brain during the first week of injury. Cortical gene expression was severely disrupted, defined by inflammation and cell death in the lesion core, and glial scar formation orchestrated by multiple cell types on the periphery. The glial scar w...
• Identification of a divalent metal transporter required for cellular iron metabolism in malaria parasites AI翻译
  作者：Loveridge, Kade M.; Sigala, Paul A.
  作者单位：Utah System of Higher Education; University of Utah
  摘要：Plasmodium falciparum malaria parasites invade and multiply inside red blood cells (RBCs), the most iron- rich compartment in humans. Like all cells, P. falciparum requires nutritional iron to support essential metabolic pathways, but the critical mechanisms of iron acquisition and trafficking during RBC infection have remained obscure. Parasites internalize and liberate massive amounts of heme during large- scale digestion of RBC hemoglobin within an acidic food vacuole (FV) but lack a heme o...
• Effects of oxycodone on placental lineages: Evidence from the transcriptome profile of mouse trophoblast giant cells AI翻译
  作者：Lyu, Zhen; Kinkade, Jessica A.; Bivens, Nathan J.; Roberts, R. Michael; Joshi, Trupti; Rosenfeld, Cheryl S.
  作者单位：University of Missouri System; University of Missouri Columbia; University of Missouri System; University of Missouri Columbia; University of Missouri System; University of Missouri Columbia; University of Missouri System; University of Missouri Columbia; University of Missouri System; University of Missouri Columbia; University of Missouri System; University of Missouri Columbia; University of Missouri System; University of Missouri Columbia; University of Missouri System; University of Missouri Columbia; University of Missouri System; University of Missouri Columbia; University of Missouri System; University of Missouri Columbia
  摘要：Pregnant women are often prescribed or abuse opioid drugs. The placenta is likely the key to understanding how opioids cause adverse pregnancy outcomes. Maternal oxycodone (OXY) exposure of pregnant mice leads to disturbances in the layer of invasive parietal trophoblast giant cells (pTGC) that forms the interface between the placenta and uterus. These cells are analogous to extravillous trophoblasts of the human placenta. They are crucial to coordinating the metabolic needs of the conceptus w...
• A CRISPR-Cas9 knockout screening identifies IRF2 as a key driver of OAS3/RNase L- mediated RNA decay during viral infection AI翻译
  作者：Oh, Sunwoo; Santiago, Gisselle; Manjunath, Lavanya; Li, Junyi; Bouin, Alexis; Semler, Bert L.; Buisson, Remi
  作者单位：University of California System; University of California Irvine; University of California System; University of California Irvine
  摘要：OAS-RNase L is a double- stranded RNA- induced antiviral pathway triggered in response to diverse viral infections. Upon activation, OAS-RNase L suppresses virus replication by promoting the decay of host and viral RNAs and inducing translational shutdown. However, whether OASs and RNase L are the only factors involved in this pathway remains unclear. Here, we develop CRISPR- Translate, a FACS-based genome-wide CRISPR-Cas9 knockout screening method that uses translation levels as a readout and...
• Reverse development in the ctenophore Mnemiopsis leidyi AI翻译
  作者：Soto-Angel, Joan J.; Burkhardt, Pawel
  作者单位：University of Bergen; University of Bergen
  摘要：Reverse development, or the ability to rejuvenate by morphological reorganization into the preceding life cycle stage is thought to be restricted to a few species within Cnidaria. To date, Turritopsis dohrnii is the only known species capable of undergoing reverse development after the onset of sexual reproduction. Here, we demonstrate that the ctenophore Mnemiopsis leidyi is capable of reversal from mature lobate to early cydippid when fed following a period of stress. Our findings illuminate...
• Is stochastic thermodynamics the key to understanding the energy costs of computation? AI翻译
  作者：Wolpert, David H.; Korbel, Jan; Lynn, Christopher W.; Tasnim, Farita; Grochow, Joshua A.; Kardes, Gulce; Aimone, James B.; Balasubramanian, Vijay; De Giuli, Eric; Doty, David; Freitas, Nahuel; Marsili, Matteo; Ouldridge, Thomas E.; Richa, Andrea W.; Riechers, Paul; Roldan, Edgar; Rubenstein, Brenda; Toroczkai, Zoltan; Paradiso, Joseph
  作者单位：The Santa Fe Institute; Arizona State University; Arizona State University-Tempe; Abdus Salam International Centre for Theoretical Physics (ICTP); Yeshiva University; Medical University of Vienna; Princeton University; City University of New York (CUNY) System; Yale University; City University of New York (CUNY) System; University of Colorado System; University of Colorado Boulder; United States Department of Energy (DOE); Sandia National Laboratories; University of Pennsylvania; University of Oxford; Toronto Metropolitan University; University of California System; University of California Davis; University of Buenos Aires; Imperial College London; Imperial College London; Nanyang Technological University; Brown University; University of Notre Dame; Massachusetts Institute of Technology (MIT)
  摘要：The relationship between the thermodynamic and computational properties of physical systems has been a major theoretical interest since at least the 19th century. It has also become of increasing practical importance over the last half-century as the energetic cost of digital devices has exploded. Importantly, real-world computers obey multiple physical constraints on how they work, which affects their thermodynamic properties. Moreover, many of these constraints apply to both naturally occurr...
• Facilitating and restraining virus infection using cell- attachable soluble viral receptors AI翻译
  作者：Zhang, Heng; Wang, Zhengli; Nguyen, Huong T. T.; Pontelli, Marjorie Cornejo; Qi, Wanrong; Rao, Liem; Liu, Zhuoming; Whelan, Sean P. J.; Zhu, Jieqing
  作者单位：Washington University (WUSTL); Medical College of Wisconsin
  摘要：SARS-CoV-2 uses the receptor binding domain (RBD) of its spike protein to recognize and infect host cells by binding to the cell surface receptor angiotensin converting enzyme 2 (ACE2). The ACE2 receptor is composed of peptidase domain (PD), collectrin-like domain, transmembrane domain, and short cytoplasmic domain, and may exist as a dimer on cell surface. The RBD binding site is located atop of the ACE2 PD, but the involvement of other domains in virus infection is uncertain. We found that t...
• The role of variable retrieval in effective learning AI翻译
  作者：Butowska-Buczynska, Ewa; Klis, Paulina; Zawadzka, Katarzyna; Hanczakowski, Maciej
  作者单位：SWPS University of Social Sciences & Humanities; Adam Mickiewicz University
  摘要：Current research on memory indicates that learning is most effective when it involves spaced retrieval practice of study materials. Here, we investigated whether the benefits of both retrieval practice and spacing can be further boosted when variability is introduced across practice sessions. Across six experiments, participants learned translations of foreign vocabulary, with foreign words embedded in contextual sentences hinting at the meaning of these words. These sentences were then either...
• O- GlcNAcylation of enolase 1 serves as a dual regulator of aerobic glycolysis and immune evasion in colorectal cancer AI翻译
  作者：Zhu, Qiang; Li, Jingchao; Sun, Haofan; Fan, Zhiya; Hu, Jiating; Chai, Siyuan; Lin, Bingyi; Wu, Liming; Qin, Weijie; Wang, Yong; Hsieh-Wilson, Linda C.; Yi, Wen
  作者单位：Zhejiang University; Zhejiang University; Zhejiang University; California Institute of Technology
  摘要：Aerobic glycolysis and immune evasion are two key hallmarks of cancer. However, how these two features are mechanistically linked to promote tumor growth is not well understood. Here, we show that the glycolytic enzyme enolase-1 (ENO1) is dynamically modified with an O- linked beta-N- acetylglucosamine (O- GlcNAcylation), and simultaneously regulates aerobic glycolysis and immune evasion via differential glycosylation. Glycosylation of threonine 19 (T19) on ENO1 promotes its glycolytic activit...
• Natural variation in age- related dopamine neuron degeneration is glutathione dependent and linked to life span AI翻译
  作者：Coleman, Colin R.; Pallos, Judit; Bustos, Alicia Arreola -; Wang, Lu; Raftery, Daniel; Promislow, Daniel E. L.; Martin, Ian
  作者单位：Oregon Health & Science University; University of Washington; University of Washington Seattle; University of Washington; University of Washington Seattle; University of Washington; University of Washington Seattle; University of Washington; University of Washington Seattle; United States Department of Agriculture (USDA); Tufts University
  摘要：Aging is the biggest risk factor for Parkinson's disease (PD), suggesting that age- related changes in the brain promote dopamine neuron vulnerability. It is unclear, however, whether aging alone is sufficient to cause significant dopamine neuron loss, and if so, how this intersects with PD- related neurodegeneration. Here, through examining a large collection of naturally varying Drosophila strains, we find a strong relationship between life span and age- related dopamine neuron loss. Strains...