2024

最新文章

• The Mitotic Checkpoint Complex controls the association of Cdc20 regulatory protein with the ubiquitin ligase APC/C in mitosis AI翻译
  作者：Sitry-Shevah, Danielle; Miniowitz-Shemtov, Shirly; Dan, Tanya Liburkin; Hershko, Avram
  作者单位：Technion Israel Institute of Technology; Rappaport Faculty of Medicine
  摘要：The ubiquitin ligase Anaphase- Promoting Complex/Cyclosome (APC/C) and its regulatory protein Cdc20 play important roles in the control of different stages of mitosis. APC/C associated with Cdc20 is active and promotes metaphase-anaphase transition by targeting for degradation inhibitors of anaphase initiation. Earlier in mitosis, premature action of APC/C is prevented by the mitotic checkpoint (or spindle assembly checkpoint) system, which ensures that anaphase is not initiated until all chro...
• Single-cell analysis via manifold fitting: A framework for RNA clustering and beyond AI翻译
  作者：Yao, Zhigang; Li, Bingjie; Lu, Yukun; Yau, Shing-Tung
  作者单位：National University of Singapore; Tsinghua University
  摘要：Single-cell RNA sequencing (scRNA-seq) data, susceptible to noise arising from biological variability and technical errors, can distort gene expression analysis and impact cell similarity assessments, particularly in heterogeneous populations. Current methods, including deep learning approaches, often struggle to accurately characterize cell relationships due to this inherent noise. To address these challenges, we introduce scAMF (Single-cell Analysis via Manifold Fitting), a framework designe...
• Postprandial cardiac hypertrophy is sustained by mechanics, epigenetic, and metabolic reprogramming in pythons AI翻译
  作者：Crocini, Claudia; Woulfe, Kathleen C.; Ozeroff, Christopher D.; Perni, Stefano; Cardiello, Joseph; Walker, Cierra J.; Wilson, Cortney E.; Anseth, Kristi; Allen, Mary Ann; Leinwand, Leslie A.
  作者单位：Free University of Berlin; Humboldt University of Berlin; Charite Universitatsmedizin Berlin; University of Colorado System; University of Colorado Boulder; University of Colorado System; University of Colorado Boulder; University of Colorado System; University of Colorado Anschutz Medical Campus; University of Colorado System; University of Colorado Anschutz Medical Campus
  摘要：Constricting pythons, known for their ability to consume infrequent, massive meals, exhibit rapid and reversible cardiac hypertrophy following feeding. Our primary goal was to investigate how python hearts achieve this adaptive response after feeding. Isolated myofibrils increased force after feeding without changes in sarcomere ultrastructure and without increasing energy cost. Ca2+ transients were prolonged after feeding with in decreased cardiac tissue stiffness. Feeding also reduced the ac...
• Unchecked growth: Pushing the limits on RNA virus genome size in the absence of known proofreading AI翻译
  作者：Glasner, Dustin R.; Daugherty, Matthew D.
  作者单位：University of California System; University of California San Diego
• Large-scale genome sequencing of giant pandas improves the understanding of population structure and future conservation initiatives AI翻译
  作者：Lan, Tianming; Yang, Shangchen; Li, Haimeng; Zhang, Yi; Li, Rengui; Sahu, Sunil Kumar; Deng, Wenwen; Liu, Boyang; Shi, Minhui; Wang, Shiqing; Du, Hanyu; Huang, Xiaoyu; Lu, Haorong; Liu, Shanlin; Deng, Tao; Chen, Jin; Wang, Qing; Han, Lei; Zhou, Yajie; Li, Qiye; Li, Desheng; Kristiansen, Karsten; Wan, Qiu - Hong; Liu, Huan; Fang, Sheng - Guo
  作者单位：Zhejiang University; Northeast Forestry University - China; Beijing Genomics Institute (BGI); Northeast Forestry University - China; Beijing Genomics Institute (BGI); Beijing Genomics Institute (BGI); Chinese Academy of Sciences; Institute of Zoology, CAS; University of Copenhagen; Beijing Genomics Institute (BGI)
  摘要：The extinction risk of the giant panda has been demoted from endangered to vulnerable on the International Union for Conservation of Nature Red List, but its habitat is more fragmented than ever before, resulting in 33 isolated giant panda populations according to the fourth national survey released by the Chinese government. Further comprehensive investigations of the genetic background and in- depth assessments of the conservation status of wild populations are still necessary and urgently n...
• Selective optogenetic inhibition of Gαq or Gαi signaling by minimal RGS domains disrupts circuit functionality and circuit formation AI翻译
  作者：Lockyer, Jayde L.; Reading, Andrew; Vicenzi, Silvia; Zbela, Agnieszka; Viswanathan, Saranya; Delandre, Caroline; Newland, Jake W.; McMullen, John P. D.; Marshall, Owen J.; Gasperini, Robert; Foa, Lisa; Lin, John Y.
  作者单位：University of Tasmania; University of Tasmania; Menzies Institute for Medical Research; University of Tasmania; University of California System; University of California San Diego
  摘要：Optogenetic techniques provide genetically targeted, spatially and temporally precise approaches to correlate cellular activities and physiological outcomes. In the nervous system, G protein- coupled receptors (GPCRs) have essential neuromodulatory functions through binding extracellular ligands to induce intracellular signaling cascades. In this work, we develop and validate an optogenetic tool that disrupts G alpha q signaling through membrane recruitment of a minimal regulator of G protein ...
• Expanded profiling of WD repeat domain 5 inhibitors reveals actionable strategies for the treatment of hematologic malignancies AI翻译
  作者：Meyer, Christian T.; Smith, Brianna N.; Wang, Jing; Teuscher, Kevin B.; Grieb, Brian C.; Howard, Gregory C.; Silver, Alexander J.; Lorey, Shelly L.; Stott, Gordon M.; Moore, William J.; Lee, Taekyu; Savona, Michael R.; Weissmiller, April M.; Liu, Qi; Quaranta, Vito; Fesik, Stephen W.; Tansey, William P.
  作者单位：University of Colorado System; University of Colorado Boulder; Vanderbilt University; Vanderbilt University; Vanderbilt University; Vanderbilt University; Vanderbilt University; Vanderbilt University; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI); Frederick National Laboratory for Cancer Research; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI); Middle Tennessee State University; Vanderbilt University; Vanderbilt University
  摘要：WD40 Repeat Domain 5 (WDR5) is a highly conserved nuclear protein that recruits MYC oncoprotein transcription factors to chromatin to stimulate ribosomal protein gene expression. WDR5 is tethered to chromatin via an arginine- binding cavity known as the WIN site. Multiple pharmacological inhibitors of the WDR5- interaction site of WDR5 (WINi) have been described, including those with picomolar affinity and oral bioavailability in mice. Thus far, however, WINi have only been shown to be effecti...
• Somatic mutations in tumor- infiltrating lymphocytes impact on antitumor immunity AI翻译
  作者：Mukohara, Fumiaki; Iwata, Kazuma; Ishino, Takamasa; Inozume, Takashi; Nagasaki, Joji; Ueda, Youki; Suzawa, Ken; Ueno, Toshihide; Ikeda, Hideki; Kawase, Katsushige; Saeki, Yuka; Kawashima, Shusuke; Yamashita, Kazuo; Kawahara, Yu; Nakamura, Yasuhiro; Tabuchi, Akiko Honobe -; Watanabe, Hiroko; Dansako, Hiromichi; Kawamura, Tatsuyoshi; Suzuki, Yutaka; Honda, Hiroaki; Mano, Hiroyuki; Toyooka, Shinichi; Kawazu, Masahito; Togashi, Yosuke
  作者单位：Okayama University; Okayama University; Chiba University; Chiba University; Chiba Cancer Center; University of Yamanashi; National Cancer Center - Japan; Chiba University; Chiba University; Saitama Medical University; University of Tokyo; Tokyo Women's Medical University
  摘要：Immune checkpoint inhibitors (ICIs) exert clinical efficacy against various types of cancers by reinvigorating exhausted CD8+T cells that can expand and directly attack cancer cells (cancer- specific T cells) among tumor- infiltrating lymphocytes (TILs). Although some reports have identified somatic mutations in TILs, their effect on antitumor immunity remains unclear. In this study, we successfully established 18 cancer- specific T cell clones, which have an exhaustion phenotype, from the TIL...
• Unexpected increase of the deuterium to hydrogen ratio in the Venus mesosphere AI翻译
  作者：Mahieux, Arnaud; Viscardy, Sebastien; Yelle, Roger Vincent; Karyu, Hiroki; Chamberlain, Sarah; Robert, Severine; Piccialli, Arianna; Trompet, Loic; Erwin, Justin Tyler; Ubukata, Soma; Nakagawa, Hiromu; Koyama, Shungo; Maggiolo, Romain; Pereira, Nuno; Cessateur, Gael; Willame, Yannick; Vandaele, Ann Carine
  作者单位：University of Arizona; University of Arizona; Tohoku University
  摘要：This study analyzes H2O and HDO vertical profiles in the Venus mesosphere using Venus Express/Solar Occultation in the InfraRed data. The findings show increasing H2O and HDO volume mixing ratios with altitude, with the D/H ratio rising significantly from 0.025 at similar to 70 km to 0.24 at similar to 108 km. This indicates an increase from 162 to 1,519 times the Earth's ratio within 40 km. The study explores two hypotheses for these results: isotopic fractionation from photolysis of H2O over...
• Cortical neurodegeneration caused by Psen1 mutations is independent of Aβ AI翻译
  作者：Yan, Kuo; Zhang, Chen; Kang, Jongkyun; Montenegro, Paola; Shen, Jie
  作者单位：Harvard University; Harvard University Medical Affiliates; Brigham & Women's Hospital; Harvard Medical School; Harvard University; Harvard Medical School; Free University of Berlin; Humboldt University of Berlin; Charite Universitatsmedizin Berlin
  摘要：Mutations in the PSEN genes are the major cause of familial Alzheimer's disease, and presenilin (PS) is the catalytic subunit of gamma- secretase, which cleaves type I trans- membrane proteins, including the amyloid precursor protein (APP) to release A f3 peptides. While PS plays an essential role in the protection of neuronal survival, PSEN mutations also increase the ratio of Af342/Af340. Thus, it remains unresolved whether PSEN mutations cause AD via a loss of its essential function or incr...