2024

最新文章

• S- adenosyl- L- methionine is the unexpected methyl donor for the methylation of mercury by the membrane- associated HgcAB complex AI翻译
  作者：Zheng, Kaiyuan; Rush, Katherine W.; Date, Swapneeta S.; Johs, Alexander; Parks, Jerry M.; Fleischhacker, Angela S.; Abernathy, Macon J.; Sarangi, Ritimukta; Ragsdale, Stephen W.
  作者单位：University of Michigan System; University of Michigan; United States Department of Energy (DOE); Oak Ridge National Laboratory; United States Department of Energy (DOE); Oak Ridge National Laboratory; Stanford University; United States Department of Energy (DOE); SLAC National Accelerator Laboratory; University of Virginia; Auburn University System; Auburn University; Vanderbilt University
  摘要：Mercury (Hg) is a heavy metal that exhibits high biological toxicity. Monomethylmercury and dimethylmercury are neurotoxins and a significant environmental concern as they bioaccumulate and biomagnify within the aquatic food web. Microbial Hg methylation involves two proteins, HgcA and HgcB. Here, we show that HgcA and HgcB can be heterologously coexpressed, and the HgcAB complex can be purified. We demonstrated that HgcA is a membrane- associated cobalamin- dependent methyltransferase and Hgc...
• Characterization of the enzyme for 5-hydroxymethyluridine production and its role in silencing transposable elements in dinoflagellates AI翻译
  作者：Li, Chongping; Li, Ying; Wang, Yuci; Meng, Xiangrui; Shi, Xiaoyan; Zhang, Yangyi; Liang, Nan; Huang, Hongda; Li, Yue; Zhou, Hui; Xu, Jiawei; Xu, Wenqi; Chen, Hao
  作者单位：Zhengzhou University; Henan Academy of Innovations in Medical Science; Southern University of Science & Technology; Southern University of Science & Technology; Fudan University
  摘要：Dinoflagellate chromosomes are extraordinary, as their organization is independent of architectural nucleosomes unlike typical eukaryotes and shows a cholesteric liquid crystal state. 5- hydroxymethyluridine (5hmU) is present at unusually high levels and its function remains an enigma in dinoflagellates chromosomal DNA for several decades. Here, we demonstrate that 5hmU contents vary among different dinoflagellates and are generated through thymidine hydroxylation. Importantly, we identified t...
• Genomic perspectives on foodborne illness AI翻译
  作者：Lipman, David J.; Cherry, Joshua L.; Strain, Errol; Agarwala, Richa; Musser, Steven M.
  作者单位：US Food & Drug Administration (FDA); National Institutes of Health (NIH) - USA; NIH National Library of Medicine (NLM); National Institutes of Health (NIH) - USA; NIH Fogarty International Center (FIC)
  摘要：Whole- genome sequencing of bacterial pathogens is used by public health agencies to link cases of food poisoning caused by the same source of contamination. The vast majority of these appear to be sporadic cases associated with small contamination episodes and do not trigger investigations. A contamination episode refers to one or more contamination events from a single source over a period of time. We examine clusters of sequenced clinical isolates of Salmonella, Escherichia coli, Campylobac...
• VEGF inhibition increases expression of HIF- regulated angiogenic genes by the RPE limiting the response of wet AMD eyes to aflibercept AI翻译
  作者：Sharma, Deepti; Lau, Evan; Qin, Yu; Jee, Kathleen; Rodrigues, Murilo; Guo, Chuanyu; Dinabandhu, Aumreetam; Mcintyre, Emma; Salman, Shaima; Hwang, Yousang; Moshiri, Ala; Semenza, Gregg L.; Montaner, Silvia; Sodhi, Akrit
  作者单位：Johns Hopkins University; Johns Hopkins Medicine; China Medical University; University System of Maryland; University of Maryland Baltimore; Johns Hopkins University; Johns Hopkins University; Johns Hopkins University; Johns Hopkins University; Johns Hopkins University; Johns Hopkins University; Johns Hopkins University; University of California System; University of California Davis
  摘要：Neovascular age- related macular degeneration (nvAMD) is the leading cause of severe vision loss in the elderly in the developed world. While the introduction of therapies targeting vascular endothelial growth factor (VEGF) has provided the first opportunity to significantly improve vision in patients with nvAMD, many patients respond inadequately to current anti-VEGF therapies. It was recently demonstrated that expression of a second angiogenic mediator, angiopoietin-like 4 (ANGPTL4), synergi...
• Profile of Catherine Drennan AI翻译
  作者：Viegas, Jennifer
• Virtual patient analysis identifies strategies to improve the performance of predictive biomarkers for PD-1 blockade AI翻译
  作者：Arulraj, Theinmozhi; Wang, Hanwen; Deshpande, Atul; Varadhan, Ravi; Emens, Leisha A.; Jaffee, Elizabeth M.; Fertig, Elana J.; Santa-Maria, Cesar A.; Popel, Aleksander S.
  作者单位：Johns Hopkins University; Johns Hopkins University; Johns Hopkins Medicine; Johns Hopkins University; Johns Hopkins University; Kaiser Permanente; Johns Hopkins University
  摘要：Patients with metastatic triple- negative breast cancer (TNBC) show variable responses to PD- 1 inhibition. Efficient patient selection by predictive biomarkers would be desirable but is hindered by the limited performance of existing biomarkers. Here, we leveraged in silico patient cohorts generated using a quantitative systems pharmacology model of metastatic TNBC, informed by transcriptomic and clinical data, to explore potential ways to improve patient selection. We evaluated and quantifie...
• Experimental coral reef communities transform yet persist under mitigated future ocean warming and acidification AI翻译
  作者：Jury, Christopher P.; Bahr, Keisha D.; Cros, Annick; Dobson, Kerri L.; Freel, Evan B.; Graham, Andrew T.; McLachlan, Rowan H.; Nelson, Craig E.; Price, James T.; de Souza, Mariana Rocha; Shizuru, Leah; Smith, Celia M.; Sparagon, Wesley J.; Squair, Cheryl A.; Timmers, Molly A.; Vicente, Jan; Webb, Maryann K.; Yamase, Nicole H.; Grottoli, Andrea G.; Toonen, Robert J.
  作者单位：University of Hawaii System; University of Hawaii Manoa; Texas A&M University System; Nature Conservancy; University of Southampton; NERC National Oceanography Centre; University System of Ohio; Ohio State University; Oregon State University; University of Hawaii System; University of Hawaii Manoa; University of Hawaii System; University of Hawaii Manoa; University of Hawaii System; University of Hawaii Manoa; University of Hawaii System; University of Hawaii Manoa; University of Hawaii System; University of Hawaii Manoa; National Geographic Society
  摘要：Coral reefs are among the most sensitive ecosystems affected by ocean warming and acidification, and are predicted to collapse over the next few decades. Reefs are predicted to shift from net accreting calcifier- dominated systems with exceptionally high biodiversity to net eroding algal- dominated systems with dramatically reduced biodiversity. Here, we present a two- year experimental study examining the responses of entire mesocosm coral reef communities to warming (+2 degrees C), acidifica...
• Characterization of RNA editing and gene therapy with a compact CRISPR-Cas13 in the retina AI翻译
  作者：Kumar, Satheesh; Hsiao, Yi - Wen; Wong, Vickie H. Y.; Aubin, Deborah; Wang, Jiang - Hui; Lisowski, Leszek; Rakoczy, Elizabeth P.; Li, Fan; Martinez, Luis Alarcon -; Cordero, Anai Gonzalez -; Bui, Bang V.; Liu, Guei-Sheung
  作者单位：Royal Victorian Eye & Ear Hospital; Centre for Eye Research Australia; University of Melbourne; University of Tasmania; Menzies Institute for Medical Research; University of Melbourne; University of Sydney; Children's Medical Research Institute - Australia; University of Sydney; Children's Medical Research Institute - Australia; University of Sydney; Military Institute of Aviation Medicine; Children's Medical Research Institute - Australia; NSW Health; Sydney Childrens Hospitals Network; University of Western Australia; Sun Yat Sen University
  摘要：CRISPR-Cas13 nucleases are programmable RNA- targeting effectors that can silence gene expression in a transient manner. Recent iterations of Cas13 nucleases are compact for adeno- associated virus (AAV) delivery to achieve strong and persistent expression of various organs in a safe manner. Here, we report significant transcriptomic signatures of Cas13bt3 expression in retinal cells and show all- in- one AAV gene therapy with Cas13bt3 can effectively silence VEGFA mRNA in human retinal organo...
• eIF4F controls ERK MAPK signaling in melanomas with BRAF and NRAS mutations AI翻译
  作者：Valcikova, Barbora; Vadovicova, Natalia; Smolkova, Karolina; Zacpalova, Magdalena; Krejci, Pavel; Lee, Shannon; Rauch, Jens; Kolch, Walter; von Kriegsheim, Alexander; Dorotikova, Anna; Andrysik, Zdenek; Vichova, Rachel; Vacek, Ondrej; Soucek, Karel; Uldrijan, Stjepan
  作者单位：Masaryk University; St. Anne's University Hospital Brno (FNUSA); St. Anne's University Hospital Brno (FNUSA-ICRC); Czech Academy of Sciences; Institute of Animal Physiology & Genetics of the Czech Academy of Sciences; University College Dublin; University College Dublin; University College Dublin; University of Edinburgh; University of Colorado System; University of Colorado Anschutz Medical Campus; Czech Academy of Sciences; Institute of Biophysics of the Czech Academy of Sciences; Masaryk University
  摘要：The eIF4F translation initiation complex plays a critical role in melanoma resistance to clinical BRAF and MEK inhibitors. In this study, we uncover a function of eIF4F in the negative regulation of the rat sarcoma (RAS)/rapidly accelerated fibrosarcoma (RAF)/mitogen- activated protein kinase kinase (MEK)/extracellular signal- regulated kinase (ERK) mitogen- activated protein kinase (MAPK) signaling pathway. We demonstrate that eIF4F is essential for controlling ERK signaling intensity in trea...
• Dramatic changes in mitochondrial subcellular location and morphology accompany activation of the CO2 concentrating mechanism AI翻译
  作者：Findinier, Justin; Joubert, Lydia - Marie; Fakhimi, Neda; Schmid, Michael F.; Malkovskiy, Andrey, V; Chiu, Wah; Burlacot, Adrien; Grossman, Arthur R.
  作者单位：Carnegie Institution for Science; Stanford University; United States Department of Energy (DOE); SLAC National Accelerator Laboratory; Stanford University; Stanford University
  摘要：Dynamic changes in intracellular ultrastructure can be critical for the ability of organisms to acclimate to environmental conditions. Microalgae, which are responsible for similar to 50% of global photosynthesis, compartmentalize their Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase (Rubisco) into a specialized structure known as the pyrenoid when the cells experience limiting CO2 conditions; this compartmentalization is a component of the CO2 Concentrating Mechanism (CCM), which facilitates ...