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作者：Marvin, Jonathan S.; Kokotos, Alexandros C.; Kumar, Mukesh; Pulido, Camila; Tkachuk, Ariana N.; Yao, Jocelyn Shuxin; Brown, Timothy A.; Ryan, Timothy A.
作者单位：Howard Hughes Medical Institute; Cornell University; Weill Cornell Medicine; Johns Hopkins University
摘要：We developed a significantly improved genetically encoded quantitative adenosine triphosphate (ATP) sensor to provide real - time dynamics of ATP levels in subcellular compartments. iATPSnFR2 is a variant of iATPSnFR1, a previously developed sensor that has circularly permuted superfolder green fluorescent protein (GFP) inserted between the ATP - binding helices of the epsilon - subunit of a bacterial F-0 - F-1 ATPase. Optimizing the linkers joining the two domains resulted in a similar to fiv...
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作者：Pattersona, James J.; Feolaa, Giuseppe; Kima, Rakhyun E.
作者单位：Utrecht University
摘要：Policy action for sustainability transformation faces inherent and ever-present sources of conflict, pushback, and resistance (i.e., discord). However, conceptual frameworks and policy prescriptions for sustainability transformations often reflect an undue image of accord. This involves simplified assumptions about consensus, steering, friction, discreteness, and additiveness of policy action, conferring an unrealistic view of the potential to deliberately realize transformation. Instead, nego...
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作者：Zhan, Junpeng; Belanger, Sebastien; Lewis, Scott; Teng, Chong; McGregor, Madison; Beric, Aleksandra; Schon, Michael A.; Nodine, Michael D.; Meyers, Blake C.
作者单位：Huazhong Agricultural University; Hubei Hongshan Laboratory; Donald Danforth Plant Science Center; James Hutton Institute; Washington University (WUSTL); University of California System; University of California Davis; University of California System; University of California Davis; University of Missouri System; University of Missouri Columbia; Wageningen University & Research
摘要：Reproductive phasiRNAs (phased, small interfering RNAs) are broadly present in angiosperms and play crucial roles in sustaining male fertility. While the premeiotic 21-nt (nucleotides) phasiRNAs and meiotic 24-nt phasiRNA pathways have been extensively studied in maize (Zea mays) and rice (Oryza sativa), a third putative category of reproductive phasiRNAs-named premeiotic 24-nt phasiRNAs-have recently been reported in barley (Hordeum vulgare) and wheat (Triticum aestivum). To determine whether...
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作者：Cui, Zepeng; Huang, Baoxuan; Zheng, Jiahao; Tian, Jia; Zhang, Weian
作者单位：East China University of Science & Technology; East China University of Science & Technology
摘要：The efficiency of photodynamic therapy (PDT) is greatly dependent on intrinsic features of photosensitizers (PSs), but most PSs suffer from narrow diffusion distances and short life span of singlet oxygen ( 1 O 2 ). Here, to conquer this issue, we propose a strategy for in situ formation of complexes between PSs and proteins to deactivate proteins, leading to highly effective PDT. The tetrafluorophenyl bacteriochlorin (FBC), a strong near - infrared absorbing photosensitizer, can tightly bind ...
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作者：Liu, Mingdong; Liu, Yunhui; Song, Tiefeng; Yang, Liuyan; Qi, Lei; Zhang, Yu- Zhong; Wang, Yong; Abcd, Qing- Tao Shen
作者单位：Southern University of Science & Technology; Southern University of Science & Technology; ShanghaiTech University; ShanghaiTech University; Zhejiang University; Shandong University; Shandong University
摘要：The endosomal sorting complexes required for transport (ESCRTs) are responsible for membrane remodeling in many cellular processes, such as multivesicular body biogenesis, viral budding, and cytokinetic abscission. ESCRT- III, the most abundant ESCRT subunit, assembles into flat spirals as the primed state, essential to initiate membrane invagination. However, the three- dimensional architecture of ESCRT- III flat spirals remained vague for decades due to highly curved filaments with a small d...
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作者：Szyszka, Taylor N.; Andreas, Micheal P.; Lie, Felicia; Miller, Lohra M.; Adamson, Lachlan S. R.; Fatehi, Farzad; Twarock, Reidun; Draper, Benjamin E.; Jarrold, Martin F.; Giessen, Tobias W.; Lau, Yu Heng
作者单位：University of Sydney; University of Sydney; University of Michigan System; University of Michigan; Indiana University System; Indiana University Bloomington; University of York - UK; University of York - UK; University of York - UK
摘要：Protein capsids are a widespread form of compartmentalization in nature. Icosahedral symmetry is ubiquitous in capsids derived from spherical viruses, as this geometry maximizes the internal volume that can be enclosed within. Despite the strong preference for icosahedral symmetry, we show that simple point mutations in a virus - like capsid can drive the assembly of unique symmetry - reduced structures. Starting with the encapsulin from Myxococcus xanthus , a 180 - mer bacterial capsid that a...
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作者：Dong, Zaizai; Wang, Yusen; Xu, Gaolian; Liu, Bing; Wang, Yang; Reboud, Julien; Jajesniak, Pawel; Yan, Shi; Ma, Pingchuan; Liu, Feng; Zhou, Yuhao; Jin, Zhiyuan; Yang, Kuan; Huang, Zhaocun; Zhuo, Minglei; Jia, Bo; Fang, Jian; Zhang, Panpan; Wu, Nan; Yang, Mingzhu; Cooper, Jonathan M.; Chang, Lingqian
作者单位：Beihang University; Beihang University; University of Glasgow; Anhui Medical University
摘要：Accurate prediction of the efficacy of immunotherapy for cancer patients through the characterization of both genetic and phenotypic heterogeneity in individual patient cells holds great promise in informing targeted treatments, and ultimately in improving care pathways and clinical outcomes. Here, we describe the nanoplatform for interrogating living cell host - gene and (micro - )environment (NICHE) relationships, that integrates micro - and nanofluidics to enable highly efficient capture of...
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作者：Sabet, Cameron John; Bajaj, Simar S.; Stanford, Fatima Cody
作者单位：Georgetown University; Harvard University; Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; Harvard University
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作者：Zhou, Jie; Le, Chau Q.; Zhang, Yun; Wells, James A.
作者单位：University of Chicago; University of Chicago; University of California System; University of California San Francisco; Chan Zuckerberg Initiative (CZI); University of California System; University of California San Francisco
摘要：Directing antibodies to a particular epitope among many possible on a target protein is a significant challenge. Here, we present a simple and general method for epitope - directed selection (EDS) using a differential phage selection strategy. This involves engineering the protein of interest (POI) with the epitope of interest (EOI) mutated using a systematic bioinformatics algorithm to guide the local design of an EOI decoy variant. Using several alternating rounds of negative selection with ...
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作者：Howard, Jeremy; Huang, Austin; Li, Zhiyuan; Tufekci, Zeynep; Zdimal, Vladimir; van der Westhuizen, Helene-Mari; von Delft, Arne; Price, Amy; Fridman, Lex; Tang, Lei-Han; Tang, Viola; Watson, Gregory L.; Bax, Christina E.; Shaikh, Reshama; Questier, Frederik; Hernandez, Danny; Chu, Larry F.; Ramirez, Christina M.; Rimoin, Anne W.
