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作者：Card, Kyle J.; Crozier, Dena; Durmaz, Arda; Gray, Jason; Creary, Justin; Stocks, Amira; Maltas, Jeff; Bonomo, Robert A.; Burke, Zachary D. C.; Scott, Jacob G.
作者单位：Cleveland Clinic Foundation; Cleveland Clinic Foundation; University System of Ohio; Case Western Reserve University; Washington University (WUSTL); University System of Ohio; Case Western Reserve University; University System of Ohio; Case Western Reserve University; Cleveland Clinic Foundation
摘要：Staphylococcus aureus bacteremia is typically treated empirically with vancomycin, with therapy later tailored based on susceptibility results. However, these tests occur before vancomycin exposure and do not account for adaptation during empiric treatment that can alter S. aureus' susceptibility to first-line drugs. To investigate these collateral drug responses, we experimentally evolved 18 methicillin-susceptible S. aureus (MSSA) populations under increasing vancomycin concentrations until ...
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作者：Rodriguez, Juan D.; Reeves, Monica N.; Chavez, Sindy R.; Wang, Hsiao- Lin V.; Chavez, Jaely Z.; Rastogi, Rhea; Sun, Liyang I.; Roberson, Mackenzie S.; Preston, Elicia A.; Massenburg, Zaynab; Cruz, Kiani N.; Chadha, Madhav S.; Hill, Emily J.; Soares, Miguel L.; Corces, Victor G.; Carpenter, Brandon S.; Schmeichel, Karen L.; Murray, John I.; Katz, David J.
作者单位：Emory University; Emory University; University System of Georgia; Kennesaw State University; University of Pennsylvania
摘要：How mutations in histone modifying enzymes lead to neurodevelopmental defects is unknown. To address this question, we took advantage of the invariant embryonic lineage and adult nervous system in Caenorhabditis elegans to investigate a double mutant between spr-5/Lsd1/Kdm1a (H3K4me1/2 demethylase) and met-2/Setdb1/Kmt1e (H3K9 methyltransferase). We demonstrate that spr-5; met-2 double mutant worms have a severe chemotaxis defect caused by the ectopic expression of germline genes in somatic ti...
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作者：Nomura, Toshihisa; Kim, June - Sik; Iwata, Osamu; Yamada, Koji; Atsuji, Kohei; Uehara-Yamaguchi, Yukiko; Yoshida, Takuhiro; Inoue, Komaki; Takahagi, Kotaro; Sakurai, Tetsuya; Shinozaki, Kazuo; Ito, Takuro; Suzuki, Kengo; Goda, Keisuke; Mochida, Keiichi
作者单位：RIKEN; RIKEN; Yamagata University; Okayama University; Yokohama City University; Kochi University; University of Tokyo; Wuhan University; University of California System; University of California Los Angeles; Tohoku University; Nagasaki University
摘要：Pre-mRNA splicing is essential for eukaryotic gene expression and is achieved through the accurate recognition of exon-intron boundaries. Although nonconventional introns, which do not follow the conventional GT-AG splicing rule, have been identified in several species, these introns are typically rare in any given genome. Here, we demonstrate the widespread occurrence of nonconventional introns (71.8% of all introns) in the Euglena agilis genome and identify consensus motifs at these nonconve...
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作者：Segerson, Kathleen
作者单位：University of Connecticut
摘要：There are a variety of contexts in which pitching in to address environmental or resource management problems could benefit from individuals or firms working not just on their own but also working together collaboratively as a group. Examples include working together on promoting conservation or habitat protection, the sharing of information, or the development of less polluting products or production processes. However, to date, the literature on environmental policy has considered only the i...
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作者：Sheng, Fang; Gao, Yu; Wang, Yaping; Li, Yang; Zhang, Ji - Ao; Zhang, Zhaoheng; Qin, Xinyan; Zhang, Shujuan; Song, Wen; Li, Jigang; Guo, Yan; Friml, Jiri; Gong, Zhizhong; Zhang, Qun; Zhang, Jing
作者单位：China Agricultural University; Naval Medical University; Chinese Academy of Agricultural Sciences; Institute of Vegetables & Flowers, CAAS; Nanjing Agricultural University; Institute of Science & Technology - Austria; Hebei University
摘要：Plants have evolved sophisticated mechanisms to adapt to environmental changes, with root gravitropism playing a pivotal role in nutrient and water acquisition. Our study reveals that SnRK2 kinases (SnRK2.2 and SnRK2.3) are critical regulators of root gravitropism through their direct phosphorylation of the auxin transporter PIN2 at S259. We demonstrate that SnRK2s- mediated phosphorylation modulates both the polar localization and transport PID, which phosphorylates PIN2 at a distinct site (S...
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作者：Chen, Xuexiang; Sun, Zejuan; Zhang, Shuhan; Lee, Gyoung - Ah; Nasu, Hiroo; Zhang, Fei; Cai, Haohong; Liu, Xu; Gao, Jixi; Zhu, Chao; Lang, Jianfeng; Zhao, Zhijun; Liu, Xinyi
作者单位：Shandong University; Shandong University; Anhui University; University of Oregon; Okayama University of Science; Chinese Academy of Social Sciences; Shandong University; Washington University (WUSTL)
摘要：Adzuki bean (Vigna angularis) is a key legume widely cultivated in East Asia, prized for both its nutritional value and nitrogen- fixing properties. This paper presents one of the oldest directly dated archaeological finds of adzuki bean, recovered from the Xiaogao site in Shandong, China, and dated to 8985- 8645 and 8032- 7939 cal. BP-predating previously known Chinese records by at least 4,000 y (approximately 6,000 y considering published directly dated evidence alone). The evidence suggest...
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作者：Lakhan, Shaheen E.; Wicks, Paul
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作者：Yin, Jie; Yang, Ke; Zhou, Chendi; Dang, Junhua
作者单位：Beijing Sport University; Beijing Sport University; Xi'an Jiaotong University; Uppsala University
摘要：Motor imagery (MI) engages neural systems that overlap with actual movement and is widely used in sport and rehabilitation. Yet conflicting findings suggest that experts show either reduced (neural efficiency) or increased (vivid simulation) brain activation during MI. We hypothesized that task complexity moderates this effect. Using fNIRS, we compared experts and novices during MI of simple versus complex soccer actions. Experts showed lower activation for simple tasks but higher activation f...
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作者：Kakade, Aishwarya Arun; Gupta, Sachin; Johnson, Andrea; Varghese, Reshmi; Adicherla, Harikrishna; Nagarkar-Jaiswal, Sonal
作者单位：Council of Scientific & Industrial Research (CSIR) - India; CSIR - Centre for Cellular & Molecular Biology (CCMB); Academy of Scientific & Innovative Research (AcSIR)
摘要：To unveil the molecular players that maintain neural stem cell (NSC) homeostasis, we conducted a genetic screen in Drosophila and isolated an uncharacterized gene that we named Inseparable (Insep). Insep is the Drosophila homologue of human IER3IP1, a gene associated with Microcephaly, Epilepsy, and Neonatal Diabetes Syndrome-1 (MEDS-1). We show that Insep loss leads to early larval lethality with small brains and these phenotypes can be rescued by expressing IER3IP1 indicating that their biol...
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作者：Korchak, Emilie J.; Sharafi, Mona; Maisonet, Isabella Jaen; Chaparro, Andres Salazar -; V. Semenova, Irina; Khan, Hamza; O'Neil, Alison L.; Caro, Pilar; Schaaf, Christian P.; Buhrlage, Sara J.; Bezsonova, Irina
作者单位：University of Connecticut; Harvard University; Harvard University Medical Affiliates; Dana-Farber Cancer Institute; Harvard University; Harvard Medical School; Wesleyan University; Wesleyan University; Ruprecht Karls University Heidelberg
摘要：Hao-Fountain syndrome is a rare neurodevelopmental disorder caused by mutations in the deubiquitinating enzyme Ubiquitin-Specific Protease 7 (USP7). Due to the novelty of the disease and its poorly understood molecular mechanisms, treatments for the syndrome are currently lacking. This study examines the effects of 11 patient-derived variants located within the catalytic domain of USP7, focusing on their impact on the enzyme's activity, thermodynamic stability, and substrate recognition. Our f...
