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最新文章

• Electron transfer in polysaccharide monooxygenase catalysis AI翻译
  作者：Sayler, Richard I.; Thomas, William C.; Rose, Alexander J.; Marletta, Michael A.
  作者单位：University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of California System; University of California Berkeley
  摘要：Polysaccharide monooxygenase (PMO) catalysis involves the chemically difficult hydroxylation of unactivated C-H bonds in carbohydrates. The reaction requires reducing equivalents and will utilize either oxygen or hydrogen peroxide as a cosubstrate. Two key mechanistic questions are addressed here: 1) How does the enzyme regulate the timely and tightly controlled electron delivery to the mononuclear copper active site, especially when bound substrate occludes the active site? and 2) How does th...
• Molecular basis of Spns1-mediated lysophospholipid transport from the lysosome AI翻译
  作者：Chen, Hongwen; Ha, Hoa T. T.; Meinhardt, Nadia Elghobashi -; Le, Nhung A.; Schmiege, Philip; Nguyen, Long N.; Li, Xiaochun
  作者单位：University of Texas System; University of Texas Dallas; University of Texas Southwestern Medical Center; National University of Singapore; University College Dublin; National University of Singapore; National University of Singapore; National University of Singapore; National University of Singapore; University of Texas System; University of Texas Southwestern Medical Center; University of Texas Dallas
  摘要：Spns1 mediates the rate- limiting efflux of lysophospholipids from the lysosome to the cytosol. Deficiency of Spns1 is associated with embryonic senescence, as well as liver and skeletal muscle atrophy in animal models. However, the mechanisms by which Spns1 transports lysophospholipid and proton sensing remain unclear. Here, we present a cryogenic electron microscopy structure of human Spns1 in lysophosphatidylcholine (LPC)-bound lumen- facing conformation. Notably, LPC snugly binds within th...
• An exchangeable SIM probe for monitoring organellar dynamics of necrosis cells and intracellular water heterogeneity in kidney repair AI翻译
  作者：Lai, Youbo; Zhang, Tengteng; Huang, Ling; Klymchenko, Andrey S.; Lin, Weiying
  作者单位：Guangxi University; Universites de Strasbourg Etablissements Associes; Universite de Strasbourg; Centre National de la Recherche Scientifique (CNRS)
  摘要：Monitoring subcellular organelle dynamics in real time and precisely assessing membrane heterogeneity in living cells are very important for studying fundamental biological mechanisms and gaining a comprehensive understanding of cellular processes. However, there remains a shortage of effective tools for these purposes. Herein, we propose a strategy to develop the exchangeable water- sensing probeAPBD for time- lapse imaging of dynamics in cellular membrane- bound organelle morphology with str...
• Energy-poverty-inequality SDGs: A large- scale household analysis and forecasting in China AI翻译
  作者：Li, Cong; Li, Minglai; Zhang, Lin; Li, Qiang; Zheng, Hua; Feldman, Marcus W.
  作者单位：Xi'an Jiaotong University; City University of Hong Kong; City University of Hong Kong; Chinese Academy of Sciences; Research Center for Eco-Environmental Sciences (RCEES), CAS; Stanford University
  摘要：Affordable and clean energy, eliminating poverty, and reducing inequality are important goals of the United Nations Sustainable Development Goals (SDGs). This paper examines the role of access to clean cooking fuels in promoting income growth and reducing income inequality. Using data from Chinese households, we show that a 10% increase in the adoption of clean cooking fuels would result in an increase in total annual household income of US$37 billion nationwide. Income growth from access to c...
• Dual- action kinase inhibitors influence p38α MAP kinase dephosphorylation AI翻译
  作者：Stadnicki, Emily J.; Ludewig, Hannes; Kumar, Ramasamy P.; Wang, Xicong; Qiao, Youwei; Kern, Dorothee; Bradshaw, Niels
  作者单位：Brandeis University; University of Massachusetts System; University of Massachusetts Worcester; Scripps Research Institute; Scripps Research Institute; Howard Hughes Medical Institute; Brandeis University; Howard Hughes Medical Institute
  摘要：Reversible protein phosphorylation directs essential cellular processes including cell division, cell growth, cell death, inflammation, and differentiation. Because protein phosphorylation drives diverse diseases, kinases and phosphatases have been targets for drug discovery, with some achieving remarkable clinical success. Most protein kinases are activated by phosphorylation of their activation loops, which shifts the conformational equilibrium of the kinase toward the active state. To turn ...
• Trojan horse peptide conjugates remodel the activity spectrum of clinical antibiotics AI翻译
  作者：Luo, Shangwen; Li, Xin- Rong; Gong, Xiao- Tong; Kulikovsky, Alexey; Qu, Feng; Beis, Konstantinos; Severinov, Konstantin; Dubiley, Svetlana; Feng, Xinxin; Dong, Shi- Hui; Nair, Satish K.
  作者单位：Lanzhou University; Russian Academy of Sciences; Institute of Gene Biology (IGB) of Russian Academy of Sciences; Imperial College London; UK Research & Innovation (UKRI); Science & Technology Facilities Council (STFC); STFC Rutherford Appleton Laboratory; Rutgers University System; Rutgers University New Brunswick; Hunan University; Hunan University; University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign
  摘要：Infections caused by gram- negative pathogens continue to be a major risk to human health because of the innate antibiotic resistance endowed by their unique cell membrane architecture. Nature has developed an elegant solution to target gram- negative strains, namely by conjugating toxic antibiotic warheads to a suitable carrier to facilitate the active import of the drug to a specific target organism. Microcin C7 (McC) is a Trojan horse peptide-conjugated antibiotic that specifically targets ...
• Family relations of Moche elite burials on the North Coast of Peru (∼500 CE): Analyses of the Senora de Cao and relatives AI翻译
  作者：Quilter, Jeffrey; Jordan, Regulo Fanco; Harkins, Kelly; Marsh, Erik; Prieto, Gabriel; Verano, John; Leblanc, Steven; Broomandkhoshbacht, Nasreen; Krigbaum, John; Fehren-Schmitz, Lars
  作者单位：Harvard University; University of California System; University of California Santa Cruz; Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET); University Nacional Cuyo Mendoza; State University System of Florida; University of Florida; Tulane University; University of California System; University of California Santa Cruz
  摘要：The Moche archaeological culture flourished along Peru's North Coast between the 4th and 10th centuries CE and was characterized by a complex social hierarchy dominated by political and religious elites. Previous archaeological evidence suggests kinship was a key factor in maintaining political authority within Moche society. To test this hypothesis, we applied archaeological, genetic, and isotopic methods to examine familial relationships between six individuals, including the prominent Senor...
• Predicting gene sequences with AI to study codon usage patterns AI翻译
  作者：Sidi, Tomer; Bahiri-Elitzur, Shir; Tuller, Tamir; Kolodny, Rachel
  作者单位：University of Haifa; Tel Aviv University; Tel Aviv University
  摘要：Selective pressure acts on the codon use, optimizing multiple, overlapping signals that are only partially understood. We trained AI models to predict codons given their amino acid sequence in the eukaryotes Saccharomyces cerevisiae and Schizosaccharomyces pombe and the bacteria Escherichia coli and Bacillus subtilis to study the extent to which we can learn patterns in naturally occurring codons to improve predictions. We trained our models on a subset of the proteins and evaluated their pred...
• ANAC044 orchestrates mitochondrial stress signaling to trigger iron- induced stem cell death in root meristems AI翻译
  作者：Yan, Juanmei; Feng, Zhihang; Xiao, Yihui; Zhou, Ming; Zhao, Xiaobo; Lin, Xianyong; Shi, Weiming; Busch, Wolfgang; Li, Baohai
  作者单位：Zhejiang University; Zhejiang University; Zhejiang University; Chinese Academy of Sciences; Nanjing Institute of Soil Science, CAS; Salk Institute
  摘要：While iron (Fe) is essential for life and plays important roles for almost all growth related processes, it can trigger cell death in both animals and plants. However, the underlying mechanisms for Fe- induced cell death in plants remain largely unknown. S- nitrosoglutathione reductase (GSNOR) has previously been reported to regulate nitric oxide homeostasis to prevent Fe- induced cell death within root meristems. Here, we found that in the absence of GSNOR, exposure to high Fe treatment resul...
• Metastable state preceding shear zone instability: Implications for earthquake- accelerated landslides and dynamic triggering AI翻译
  作者：Li, Yan; Hu, Wei; Xu, Qiang; Luo, Hui; Chang, Chingshung; Jia, Xiaoping
  作者单位：Chengdu University of Technology; University of Massachusetts System; University of Massachusetts Amherst; Universite PSL; Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI); Centre National de la Recherche Scientifique (CNRS); Universite Paris Cite
  摘要：Understanding the dynamic response of granular shear zones under cyclic loading is fundamental to elucidating the mechanisms triggering earthquake- induced landslides, with implications for broader fields such as seismology and granular physics. Existing prediction methods struggle to accurately predict many experimental and in situ landslide observations due to inadequate consideration of the underlying physical mechanisms. The mechanisms that influence landslide dynamic triggering, a transit...