7期

最新文章

• AI protocol for retrieving protein dynamic structures from two- dimensional infrared spectra AI翻译
  作者：Ye, Sheng; Zhu, Lvshuai; Zhao, Zhicheng; Wu, Fan; Li, Zhipeng; Wang, Binbin; Zhong, Kai; Sun, Changyin; Mukamel, Shaul; Jiang, Jun
  作者单位：Anhui University; Chinese Academy of Sciences; University of Science & Technology of China, CAS; University of Groningen; University of California System; University of California Irvine; University of California System; University of California Irvine
  摘要：Understanding the dynamic evolution of protein structures is crucial for uncovering their biological functions. Yet, real- time prediction of these dynamic structures remains a significant challenge. Two- dimensional infrared (2DIR) spectroscopy is a powerful tool for analyzing protein dynamics. However, translating its complex, low- dimensional signals into detailed three- dimensional structures is a daunting task. In this study, we introduce a machine learning- based approach that accurately...
• Ratio- based indicators for cytosolic Ca2+with visible light excitation AI翻译
  作者：Zhou, Xinqi; Belavek, Kayla J.; Navarro, Marisol X.; Martinez, Kayli N.; Hinojosa, Abigail; Miller, Evan W.
  作者单位：University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of California System; University of California Berkeley
  摘要：Calcium ions (Ca2+) play central roles in cellular physiology. Fluorescent indicators for Ca2+ ions revolutionized our ability to make rapid, accurate, and highly parallel measurement of Ca2+ concentrations in living cells. The use of ratio- based imaging with one particular indicator, fura- 2, allowed practitioners to correct for a number of experimental confounds, including dye bleaching, variations in sample thickness, and fluctuations in illumination intensity. Ratio- based imaging with fu...
• Interconnecting fragmented forests: Small and mobile birds are cornerstones in the plant-frugivore meta- network AI翻译
  作者：Zhu, Chen; Dalsgaard, Bo; Li, Wande; Kaiser-Bunbury, Christopher N.; Simmons, Benno I.; Ren, Peng; Zhao, Yuhao; Zeng, Di; Goncalves, Fernando; Zhang, Xue; Chang, Longxiao; Ding, Ping; Si, Xingfeng
  作者单位：Zhejiang University; University of Copenhagen; East China Normal University; University of Exeter; University of Zurich
  摘要：Habitat fragmentation is causing the collapse of seed dispersal interactions and ecosystem functioning. When management and conservation strategies aim to sustain ecosystem functioning of fragmented forests, species' traits and functional performance are critical in guiding decisions. However, to date, we lack a quantitative understanding of the role of frugivores' body size and dispersal ability in ecosystem sustainability among fragmented forests. Focusing on avian frugivory and seed dispers...
• Substance P receptor signaling contributes to host maladaptive responses during enteric bacterial infection AI翻译
  作者：Cremin, Michael; Ramirez, Valerie T.; Sanchez, Kristina; Tay, Emmy; Murray, Kaitlin; Brust-Mascher, Ingrid; Reardon, Colin
  作者单位：University of California System; University of California Davis
  摘要：Immune responses in the intestine are intricately balanced to prevent pathogen entry without inducing immunopathology. The nervous system is well established to interface with the immune system to fine- tune immunity in various organ systems including the gastrointestinal tract. Specialized sensory neurons can detect bacteria, bacterial products, and the resulting inflammation, to coordinate the immune response in the gastrointestinal tract. These sensory neurons release peptide neurotransmitt...
• Identification of a protective antigen reveals the trade- off between iron acquisition and antigen exposure in a global fungal pathogen AI翻译
  作者：Li, Yeqi; Pham, Tuyetnhu; Hipsher, Kenton; Lee, Christopher W. J.; Jiao, Jie; Penninger, Josef M.; Kronstad, James W.; Fan, Yumeng; Zhao, Youbao; Ambati, Suresh; Meagher, Richard B.; Xie, Xiaofeng; Lin, Xiaorong
  作者单位：University System of Georgia; University of Georgia; University System of Georgia; University of Georgia; University of British Columbia; University of British Columbia; Medical University of Vienna; Helmholtz Association; Helmholtz-Center for Infection Research; Austrian Academy of Sciences; Vienna Biocenter (VBC); Institute of Molecular Biotechnology (IMBA); University System of Georgia; University of Georgia
  摘要：Systemic infections caused by Cryptococcus claim over 161,000 lives annually, with global mortality rate close to 70% despite antifungal therapies. Currently, no vaccine is available. To develop an effective multivalent vaccine against this free- living oppor-tunistic eukaryotic pathogen, it is critical to identify protective antigens. We previously discovered ZNF2oe strains elicit protective host immune responses and increase the abundance of antigens present in the capsule, which is required...
• Computational and in vitro evaluation of probiotic treatments for nasal Staphylococcus aureus decolonization AI翻译
  作者：Tepekule, Burcu; Barcik, Weronika; Staiger, Willy I.; Bergada-Pijuan, Judith; Scheier, Thomas; Brulisauer, Laura; Hall, Alex R.; Gunthard, Huldrych F.; Hilty, Markus; Kouyos, Roger D.; Brugger, Silvio D.
  作者单位：University of Zurich; University Zurich Hospital; University of Zurich; University Zurich Hospital; University of Zurich; Swiss Federal Institutes of Technology Domain; ETH Zurich; University of Bern
  摘要：Despite the rising challenge of antibiotic resistance, current approaches to eradicate nasal pathobionts Staphylococcus aureus and Streptococcus pneumoniae rely on antibac-terials. An alternative is the artificial inoculation of commensal bacteria, i.e., probiotic treatment, supported by the increasing evidence for commensal- mediated inhibition of pathogens. To systematically investigate the potential of this approach, we developed a quantitative framework simulating the nasal microbiome dyna...
• Structural basis of disease mutation and substrate recognition by the human SLC2A9 transporter AI翻译
  作者：Khandelwal, Nitesh Kumar; Gupta, Meghna; Kumar, Paras; Balasubramani, Sree Ganesh; Echeverria, Ignacia; Stroud, Robert M.
  作者单位：University of California System; University of California San Francisco; University of California System; University of California San Francisco; University of California System; University of California San Francisco; Oregon Health & Science University
  摘要：Urate provides similar to 50% of the reducing potential in human and primate plasma which is key to detoxifying reactive oxygen by- products of cellular metabolism. Urate is the endpoint of purine metabolism in primates, and its concentration in plasma is a bal-ance between excretion from kidney and intestine, and subsequent reabsorption in and through cells of kidney proximal tubules to maintain a regulated concentration in plasma. SLC2A9 is the primary transporter that returns urate from the...
• The canonical RPA complex interacts with Est3 to regulate yeast telomerase activity AI翻译
  作者：Moeller-McCoy, Corinne A.; Wieser, Thomas A.; Lubin, Johnathan W.; Gillespie, Abigail E.; Ramirez, Jocelyn A.; Paschini, Margherita; Wuttke, Deborah S.; Lundblad, Victoria
  作者单位：Salk Institute; University of California System; University of California San Diego; University of Colorado System; University of Colorado Boulder; University of California System; University of California San Diego; University of Colorado System; University of Colorado Anschutz; Harvard University; Harvard University Medical Affiliates; Boston Children's Hospital; Harvard University; Harvard University Medical Affiliates; Boston Children's Hospital; Harvard University; Harvard University Medical Affiliates; Boston Children's Hospital
  摘要：In most eukaryotic organisms, cells that rely on continuous cell division employ the enzyme telomerase which replenishes chromosome termini through the addition of telomeric repeats. In budding yeast, the telomerase holoenzyme is composed of a catalytic core associated with two regulatory subunits, Est1 and Est3. The Est1 protein binds a telomere- specific RPA-like complex to recruit telomerase to chromosome ends. However, the regulatory function of the Est3 subunit has remained elusive. We re...
• Astrocytic EphA4 signaling is important for the elimination of excitatory synapses in Alzheimer's disease AI翻译
  作者：Yang, Xin; Wang, Ye; Qiao, Yi; Lin, Jingwen; Lau, Jackie K. Y.; Fu, Wing-Yu; Fu, Amy K. Y.; Ip, Nancy Y.
  作者单位：Hong Kong University of Science & Technology; Hong Kong University of Science & Technology
  摘要：Cell surface receptors, including erythropoietin- producing hepatocellular A4 (EphA4), are important in regulating hippocampal synapse loss, which is the key driver of memory decline in Alzheimer's disease (AD). However, the cell- specific roles and mechanisms of EphA4 are unclear. Here, we show that EphA4 expression is elevated in hippocampal CA1 astrocytes in AD conditions. Specific knockout of astrocytic EphA4 ameliorates excitatory synapse loss in the hippocampus in AD transgenic mouse mod...
• Double assurance in the induction of axial development by egg dorsal determinants in Xenopus embryos AI翻译
  作者：Azbazdar, Yagmur; De Robertis, Edward M.
  作者单位：University of California System; University of California Los Angeles; David Geffen School of Medicine at UCLA; University of California Los Angeles Medical Center
  摘要：We recently reported that microinjection of Xenopus nodal- related (xnr) mRNAs into beta- catenin- depleted Xenopus embryos rescued a complete dorsal axis. Xnrs mediate the signal of the Nieuwkoop center that induces the Spemann-Mangold organizer in the overlying mesoderm, a process inhibited by the Nodal antagonist Cerberus-short (CerS). However, beta- catenin also induces a second signaling center in the dorsal prospective ectoderm, designated the Blastula Chordin and Noggin Expression (BCNE...