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作者：Chen, Dahong; Keremane, Saumya; Wang, Silu; Lei, Elissa P.
作者单位：National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK); State University of New York (SUNY) System; University at Buffalo, SUNY; State University of New York (SUNY) System; University at Buffalo, SUNY
摘要：Chromatin architecture facilitates accurate transcription at a number of loci, but it remains unclear how much chromatin architecture is involved in global transcriptional regulation. Previous work has shown that rapid depletion of the architectural protein CTCF in cell culture alters global chromatin organization but results in surprisingly limited gene expression changes. This discrepancy has also been observed when other architectural proteins are depleted, and one possible explanation is t...
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作者：Gilles, Marc Aurele; Singer, Amit
作者单位：Princeton University; Princeton University
摘要：Proteins and the complexes they form are central to nearly all cellular processes. Their flexibility, expressed through a continuum of states, provides a window into their biological functions. Cryogenic electron microscopy (cryo-EM) is an ideal tool to study these dynamic states as it captures specimens in noncrystalline conditions and enables high-resolution reconstructions. However, analyzing the heterogeneous distributions of conformations from cryo-EM data is challenging. We present RECOV...
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作者：Szathmary, Ears
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作者：Cvijovic, Ivana; Swift, Michael; Quake, Stephen R.
作者单位：Stanford University; Stanford University; Stanford University; Chan Zuckerberg Initiative (CZI)
摘要：B cells generate pathogen-specific antibodies and play an essential role in providing adaptive protection against infection. Antibody genes are modified in evolutionary processes acting on the B cell populations within an individual. These populations proliferate, differentiate, and migrate to long-term niches in the body. However, the dynamics of these processes in the human immune system are primarily inferred from mouse studies. We addressed this gap by sequencing the antibody repertoire an...
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作者：Xiao, Lifang; Chen, Liang; Labandeira, Conrad C.; Azevedo-Schmidt, Lauren; Wang, Yongjie; Ren, Dong
作者单位：Guangdong Academy of Sciences; Institute of Zoology, Guangdong Academy of Sciences; Capital Normal University; Smithsonian Institution; Smithsonian National Museum of Natural History; University System of Maryland; University of Maryland College Park; University System of Maryland; University of Maryland College Park; University of California System; University of California Davis
摘要：Modern ecosystems display complex associations of plants-insects that underwent a long evolutionary process since the appearance of mid- Paleozoic vascular plants. Although several major hypotheses explain the evolution of these plant-insect associations, the initial pattern of modern insect herbivory is poorly understood. To understand the antiquity of modern patterns of terrestrial arthropod herbivory, functional feeding group-damage type (FFG- DT) data were used to analyze a 305 My interval...
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作者：Tamborini, Alessio; Aghilinejad, Arian; Gharib, Morteza
作者单位：California Institute of Technology
摘要：Analyzing cardiac pulse waveforms offers valuable insights into heart health and cardiovascular disease risk, although obtaining the more informative measurements from the central aorta remains challenging due to their invasive nature and limited noninvasive options. To address this, we employed a laboratory- developed cuff device for high- resolution pulse waveform acquisition and constructed a spectral machine learning model to nonlinearly map the brachial wave components to the aortic site....
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作者：Cheng, Xuanbing; Li, Zongqi; Zhu, Jialun; Wang, Jingyu; Huang, Ruyi; Yu, Lewis W.; Lin, Shuyu; Forman, Sarah; Gromilina, Evelina; Puri, Sameera; Patel, Pritesh; Bahramian, Mohammadreza; Tan, Jiawei; Hojaiji, Hannaneh; Jelinek, David; Voisin, Laurent; Yu, Kristie B.; Zhang, Ao; Ho, Connie; Lei, Lei; Coller, Hilary A.; Hsiao, Elaine Y.; Reyes, Beck L.; Matsumoto, Joyce H.; Lu, Daniel C.; Liu, Chong; Milla, Carlos; Davis, Ronald W.; Emaminejad, Sam
作者单位：University of California System; University of California Los Angeles; University of California System; University of California Los Angeles; University of California System; University of California Los Angeles; University of California System; University of California Los Angeles; University of California Los Angeles Medical Center; David Geffen School of Medicine at UCLA; University of California System; University of California Los Angeles; University of California Los Angeles Medical Center; David Geffen School of Medicine at UCLA; University of California System; University of California Los Angeles; University of California System; University of California Los Angeles; University of California System; University of California Los Angeles; University of California System; University of California Los Angeles; University of California System; University of California Los Angeles; University of California System; University of California Los Angeles; University of California System; University of California Los Angeles; University of California Los Angeles Medical Center; David Geffen School of Medicine at UCLA; University of California System; University of California Los Angeles; University of California System; University of California Los Angeles; Stanford University; Stanford University; University of California System; University of California Los Angeles
摘要：Mimicking metabolic pathways on electrodes enables in vivo metabolite monitoring for decoding metabolism. Conventional in vivo sensors cannot accommodate underlying complex reactions involving multiple enzymes and cofactors, addressing only a fraction of enzymatic reactions for few metabolites. We devised a single- wall- carbon- nanotube- electrode architecture supporting tandem metabolic pathway-like reactions linkable to oxidoreductase- based electrochemical analysis, making a vast majority ...
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作者：Meng, Luming; Sheong, Fu Kit; Luo, Qiong
作者单位：South China Agricultural University; Guangdong Laboratory for Lingnan Modern Agriculture; Hong Kong University of Science & Technology; South China Normal University
摘要：DNA is heterogeneously packaged into chromatin, which is further organized into topologically associating domains (TADs) with sharp boundaries. These boundary locations are critical for genome regulation. Here, we explore how the distribution of DNA- packing density across chromatin affects the TAD boundary locations. We develop a polymer- physics- based model that utilizes DNA accessibility data to parameterize DNA- packing density along chromosomes, treating them as heteropolymers, and simul...
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作者：Oluoch, Peter O.; Koh, Eun- Ik; Proulx, Megan K.; Reames, Charlotte J.; Papavinasasundaram, Kadamba G.; Murphy, Kenan C.; Zimmerman, Matthew D.; Dartois, Veronique; Sassetti, Christopher M.
作者单位：University of Massachusetts System; University of Massachusetts Worcester
摘要：Successful tuberculosis therapy requires treatment with an unwieldy multidrug combi- nation for several months. Thus, there is a growing need to identify novel genetic vul- nerabilities that can be leveraged to develop new, more effective antitubercular drugs. Consequently, recent efforts to optimize tuberculosis (TB) therapy have exploited Mycobacterium tuberculosis (Mtb) chemical genetics to identify pathways influencing antibiotic efficacy, novel mechanisms of antibiotic action, and new tar...
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作者：Atlas, Rachel; Podglajen, Aurelien; Wilson, Richard; Hertzog, Albert; Plougonven, Riwal
