19期

最新文章

• Hdac1 as an early determinant of intermediate- exhausted CD8+T cell fate in chronic viral infection AI翻译
  作者：Hu, Wei; Hu, Shengen Shawn; Zhu, Shaoqi; Peng, Weiqun; Badovinac, Vladimir P.; Zang, Chongzhi; Zhao, Xudong; Xue, Hai-Hui
  作者单位：Hackensack University Medical Center; University of Virginia; University of Virginia; George Washington University; Sun Yat Sen University; University of Iowa; China Medical University
  摘要：The exhausted CD8+ T (TEX) cells consist of distinct subsets including Tcf1+ stem-like, Tcf1-Cx3cr1+ intermediate (TEX-int) and Tcf1-Cx3cr1-terminally exhausted cells; yet, epigenetic determinants of TEX subset differentiation remain incompletely understood. Using chronic viral infection, we show that histone deacetylase 1 (Hdac1) was specifically required for the formation of antigen-specific TEX-int cells at the effector phase of responses. Single-cell transcriptomics validated that Hdac1 de...
• Combining live fluorescence imaging with in situ cryoelectron tomography sheds light on the septation process in Deinococcus radiodurans AI翻译
  作者：Gaifas, Lorenzo; Kleman, Jean - Philippe; Lacroix, Francoise; Schexnaydre, Erin; Trouve, Jennyfer; Morlot, Cecile; Sandblad, Linda; Gutsche, Irina; Timmins, Joanna
  作者单位：Communaute Universite Grenoble Alpes; Universite Grenoble Alpes (UGA); CEA; Centre National de la Recherche Scientifique (CNRS); Umea University; Umea University
  摘要：Cell division is a fundamental biological process that allows a single mother cell to produce two daughter cells. In walled bacteria, different modes of cell division have been reported that are notably associated with distinctive cell shapes. In all cases, division involves a step of septation, corresponding to the growth of a new dividing cell wall, followed by splitting of the two daughter cells. The radiation-resistant Deinococcus radiodurans is a spherical bacterium protected by a thick a...
• Directional water navigation and reallocation in Tillandsia capitata AI翻译
  作者：Lian, Jiaoyuan; Li, Wei; Yang, Ling; Li, Hegeng; Deng, Qiyu; Zhu, Hengjia; Zhang, Yiyuan; Fang, Nicholas X.; Wang, Liqiu
  作者单位：University of Hong Kong; Hong Kong Polytechnic University
  摘要：Liquid manipulation is ubiquitous in nature and engineering, enabling controllable and efficient liquid delivery. Conventional understanding of liquid manipulation relies on inhomogeneous chemical modifications or single-scale structure design. Here, we present how water is directionally navigated and spontaneously reallocated at high efficiency via the cross-scale topology on Tillandsia capitata leaves. These leaves feature transversely curved lanceolate macrostructures decorated by a layer o...
• Acute chromatin decompaction stiffens the nucleus as revealed by nanopillar- induced nuclear deformation in cells AI翻译
  作者：Mitra, Aninda; Cutiongco, Marie F. A.; Burla, Romina; Zeng, Yongpeng; Na, Qin; Kong, Mengya; Vinod, Benjamin; Nai, Mui Hoon; Hubner, Barbara; Ludwig, Alexander; Lim, Chwee Teck; Shivashankar, G. V.; Saggio, Isabella; Zhao, Wenting
  作者单位：Nanyang Technological University; Sapienza University Rome; National University of Singapore; Nanyang Technological University; Nanyang Technological University; National University of Singapore; National University of Singapore; Swiss Federal Institutes of Technology Domain; ETH Zurich; Swiss Federal Institutes of Technology Domain; Paul Scherrer Institute; Nanyang Technological University
  摘要：Chromatin architecture is critical in determining nuclear mechanics. Most studies focus on the mechanical rigidity conferred by chromatin condensation from densely packed heterochromatin, but less is known on how transient chromatin decompaction impinge on nucleus stiffness. Here, we used an array of vertically aligned nanopillars to study nuclear deformability in situ after chromatin decompaction in cells. The nucleus significantly stiffened within 4 h of chromatin decompaction but softened a...
• Cross-feeding creates tipping points in microbiome diversity AI翻译
  作者：Clegg, Tom; Gross, Thilo
  作者单位：Carl von Ossietzky Universitat Oldenburg; Helmholtz Association; Alfred Wegener Institute, Helmholtz Centre for Polar & Marine Research; Carl von Ossietzky Universitat Oldenburg
  摘要：A key unresolved question in microbial ecology is how the extraordinary diversity of microbiomes emerges from the interactions among their many functionally distinct populations. This process is driven in part by the cross-feeding networks that help to structure these systems, in which consumers use resources to fuel their metabolism, creating by-products which can be used by others in the community. Understanding the effects of cross-feeding presents a major challenge, as it creates complex i...
• The oncoprotein SET promotes serine-derived one-carbon metabolism by regulating SHMT2 enzymatic activity AI翻译
  作者：Jiao, Zishan; Zhang, Mi; Ning, Jingyuan; Yao, Han; Yan, Xiaojun; Wu, Zhen; Wu, Dexuan; Liu, Yajing; Zhang, Meng; Wang, Lin; Wang, Donglai
  作者单位：Chinese Academy of Medical Sciences - Peking Union Medical College; Peking Union Medical College; Chinese Academy of Medical Sciences - Peking Union Medical College; Peking Union Medical College; Chinese Academy of Medical Sciences - Peking Union Medical College; Peking Union Medical College; China Medical University; Chinese Academy of Medical Sciences - Peking Union Medical College; Peking Union Medical College
  摘要：Cancer cells frequently reprogram one-carbon metabolic pathways to fulfill their vigorous demands of biosynthesis and antioxidant defense for survival and proliferation. Dysfunction of oncogenes or tumor suppressor genes is critically involved in this process, but the precise mechanisms by which cancer cells actively trigger one-carbon metabolic alterations remain incompletely elucidated. Here, by using untargeted metabolomic analysis, we identify the oncoprotein SE translocation (SET) as a ke...
• Blocking C-terminal processing of KRAS4b via a direct covalent attack on the CaaX-box cysteine AI翻译
  作者：Maciag, Anna E.; Yang, Yue; Sharma, Alok K.; Turner, David M.; Dehart, Caroline J.; Abdelkarim, Hazem; Fan, Lixin; Smith, Brian P.; Kumari, Vandana; Dyba, Marcin; Rigby, Megan; Badillo, Jean A. Castillo; Adams, Lauren; Fornelli, Luca; Fox, Stephen; Brafman, Alla; Turbyville, Thomas; Gillette, William; Messing, Simon; Agamasu, Constance; Wolfe, Andrew L.; Gysin, Stephan; Chan, Albert H.; Simanshu, Dhirendra K.; Esposito, Dominic; Chertov, Oleg; Stephen, Andrew G.; Arkin, Michelle; Renslo, Adam; Kelleher, Neil L.; Gaponenko, Vadim; Lightstone, Felice C.; Nissley, Dwight, V; McCormick, Frank
  作者单位：National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI); Frederick National Laboratory for Cancer Research; United States Department of Energy (DOE); Lawrence Livermore National Laboratory; University of Illinois System; University of Illinois Chicago; University of Illinois Chicago Hospital; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI); Frederick National Laboratory for Cancer Research; Northwestern University; Northwestern University; University of California System; University of California San Francisco; UCSF Medical Center; UCSF Helen Diller Family Comprehensive Cancer Center; University of California System; University of California San Francisco; Northwestern University; Northwestern University; Northwestern University
  摘要：RAS is the most frequently mutated oncogene in cancer. RAS proteins show high sequence similarities in their G-domains but are significantly different in their C-terminal hyper-variable regions (HVR). These regions interact with the cell membrane via lipid anchors that result from posttranslational modifications (PTM) of cysteine residues. KRAS4b is unique as it has only one cysteine that undergoes PTM, C185. Small molecule covalent modification of C185 would block any form of prenylation and ...
• Exploiting thioether reactivity to label mycobacterial glycans AI翻译
  作者：Smelyansky, Stephanie R.; Ma, Chi - Wang; Marando, Victoria M.; Babunovic, Gregory H.; Lee, So Young; Bryson, Bryan D.; Kiessling, Laura L.
  作者单位：Massachusetts Institute of Technology (MIT); Harvard University; Massachusetts Institute of Technology (MIT); Ragon Institute; Harvard University; Harvard University Medical Affiliates; Brigham & Women's Hospital; Massachusetts Institute of Technology (MIT); Harvard University; Massachusetts Institute of Technology (MIT); Broad Institute; University of Wisconsin System; University of Wisconsin Madison
  摘要：Mycobacterium tuberculosis (Mtb) is a leading cause of death worldwide. Mtb cell envelope glycans are potent virulence factors that play key roles in mediating infection of host tissues and modulating the host immune response. However, there are few ways to site-selectively modify and label these or any glycans to study their functions in disease. This gap arises because glycans generally lack functional groups amenable to bioconjugation strategies. Methylthioxylofuranose (MTX), a rare monosac...
• Structure of the human TWIK-2 potassium channel and its inhibition by pimozide AI翻译
  作者：Khanra, Nandish K.; Wang, Chongyuan; Delgado, Bryce D.; Long, Stephen B.
  作者单位：Memorial Sloan Kettering Cancer Center; Cornell University; Weill Cornell Medicine; Chinese Academy of Sciences; Shenzhen Institute of Advanced Technology, CAS; Roche Holding; Roche Holding USA; Genentech
  摘要：The potassium channel TWIK-2 is crucial for ATP-induced activation of the NLRP3 inflammasome in macrophages. The channel is a member of the two-pore domain potassium (K2P) channel superfamily and an emerging therapeutic target to mitigate severe inflammatory injury involving NLRP3 activation. We report the cryo-EM structure human TWIK-2. In comparison to other K2P channels, the structure reveals an unusual up conformation of Tyr111 in the selectivity filter and a resulting SF1-P1 pocket behind...
• OGG1S326C variant frequent in human populations facilitates inflammatory responses due to its extended interaction with DNA substrate AI翻译
  作者：Han, Jinling; Zhang, Meichen; Ge, Jiakun; Ji, Zhihua; Zhao, Jianyi; Hu, Yinchao; Li, Chunshuang; Xue, Yaoyao; Li, Xining; Zhao, Haiwang; Cui, Zixu; Tian, Miaomiao; Zheng, Xu; Wang, Dapeng; Wang, Jing; Wei, Min; Radak, Zsolt; Nakabeppu, Yusaku; Boldogh, Istvan; Ba, Xueqing
  作者单位：Northeast Normal University - China; Northeast Normal University - China; Jilin University; Jiangnan University; Nanjing Medical University; University of Physical Education; Kyushu University; University of Texas System; University of Texas Medical Branch Galveston
  摘要：8-oxoguanine (8-oxoGua) is one of the most frequent forms of oxidative DNA base lesions, repaired by 8-oxoguanine DNA glycosylase 1 (OGG1) via base excision repair (BER) pathway to maintain genome fidelity. The human allelic variant hOGG1S326C, prevalent in Caucasians and Asians, has been regarded as a susceptibility factor for various diseases, yet its pathogenic mechanism remains elusive. In this study, we demonstrate that Ogg1S326C/S326Cmice exhibit increased and sustained airway inflammati...