19期

最新文章

• DDX24 spatiotemporally orchestrates VEGF and Wnt signaling during developmental angiogenesis AI翻译
  作者：Chen, Fangbin; Deng, Zhaohua; Wang, Xiaoming; Liu, Yuxuan; Zhao, Kaichen; Zhang, Yue; He, Simeng; Ran, Rensen; Dong, Yingying; Guo, Shuang; Zhou, Yitong; Zhou, Bin; Pang, Pengfei; Ge, Wei; Liu, Chang; Shan, Hong; He, Huanhuan
  作者单位：Sun Yat Sen University; Sun Yat Sen University; Wuhan University; Huazhong Agricultural University; University of Macau; Beijing Genomics Institute (BGI); Shanxi Medical University; Beijing Genomics Institute (BGI)
  摘要：Vascular development is a precisely controlled process, yet how it is spatiotemporally orchestrated remains enigmatic. We previously identified DEAD-box RNA helicase 24 (DDX24) as a pathogenic gene for multiorgan vascular anomalies. Here, we show that DDX24 is expressed in the endothelium during embryonic angiogenesis in zebrafish. DDX24 deficiency causes intersegmental vessel hyperbranching in the trunk, but inhibits central artery angiogenesis in the brain. Mechanistically, DDX24 deficiency ...
• Quantitative spatial analysis of chromatin biomolecular condensates using cryoelectron tomography AI翻译
  作者：Zhou, Huabin; Hutchings, Joshua; Shiozaki, Momoko; Zhao, Xiaowei; Doolittle, Lynda K.; Yang, Shixin; Yan, Rui; Jean, Nikki; Riggi, Margot; Yu, Zhiheng; Villa, Elizabeth; Rosen, Michael K.
  作者单位：University of Texas System; University of Texas Southwestern Medical Center; Howard Hughes Medical Institute; University of Texas System; University of Texas Southwestern Medical Center; University of California System; University of California San Diego; Howard Hughes Medical Institute; Max Planck Society; University of California System; University of California San Diego; Howard Hughes Medical Institute
  摘要：Phase separation is an important mechanism to generate certain biomolecular condensates and organize the cell interior. Condensate formation and function remain incompletely understood due to difficulties in visualizing the condensate interior at high resolution. Here, we analyzed the structure of biochemically reconstituted chromatin condensates through cryoelectron tomography. We found that traditional blotting methods of sample preparation were inadequate, and high- pressure freezing plus f...
• Synergistic regulation of metal-organic cage architectures via temperature- and solvent-driven atropisomerism AI翻译
  作者：Liang, Jiaqi; Peng, Li-Jun; Zhu, Ke-Lin; Li, Zhi-Ao; Chen, Xu-Lang; Yang, Yu-Dong; Li, Qian; Bi, Qian-Nan; Cui, Jie; Guan, Ai-Jiao; Liang, Tong-Ling; Hao, Xiang; Wang, Heng; Li, Xiaopeng; Gong, Han-Yuan
  作者单位：Beijing Normal University; Hubei Normal University; University of Texas System; University of Texas Austin; Shenzhen University; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Shanghai Institute of Organic Chemistry, CAS
  摘要：Regulating multistimulus responses in artificial systems remains a challenge in smart material development. We present a versatile chemical switching system that precisely controls the self-assembly of metal-organic cages via temperature and solvent changes. The key component, cyclo[2](1,3-(4,6-dimethyl)benzene) (4-pyridine)[6](1,3-(4,6-dimethyl)benzene) (CP2), was generated as three atropisomers (1, 2, and 3) with Cs, C1, and C2v symmetries. Thermally, metastable isomers (1 and 2) convert int...
• Cyclic peptide inhibitors function as molecular glues to stabilize Gq/ 11 heterotrimers AI翻译
  作者：Muhle, Jonas; Alenfelder, Judith; Rodrigues, Matthew J.; Juergenliemke, Lars; Guixa-Gonzalez, Ramon; Graetz, Lukas; Andres, Fabio; Bacchin, Arianna; Hennig, Michael; Schihada, Hannes; Cruesemann, Max; Koenig, Gabriele M.; Schertler, Gebhard; Kostenis, Evi; Deupi, Xavier
  作者单位：University of Bonn; University of Bonn; Swiss Institute of Bioinformatics; Consejo Superior de Investigaciones Cientificas (CSIC); CSIC - Centro de Investigacion y Desarrollo Pascual Vila (CID-CSIC); CSIC - Instituto de Quimica Avanzada de Cataluna (IQAC); Philipps University Marburg; University of Bonn; Goethe University Frankfurt
  摘要：Heterotrimeric G alpha:Gfa gamma G proteins function as molecular switches downstream of G protein-coupled receptors (GPCRs). They alternate between a heterotrimeric GDP- bound OFF- state and a GTP- bound ON- state in which G alpha GTP is separated from the Gfa gamma dimer. Consequently, pharmacological tools to securely prevent the OFF- ON transition are of utmost importance to investigate their molecular switch function, specific contribution to GPCR signal transduction, and potential as dru...
• Unbiased mechanical cloaks AI翻译
  作者：Senhora, Fernando Vasconcelos; Sanders, Emily D.; Paulino, Glaucio H.
  作者单位：University System of Georgia; Georgia Institute of Technology; University System of Georgia; Georgia Institute of Technology; Princeton University; Princeton University
  摘要：The distinction between reinforcement and cloaking has been overlooked in optimization-based design of devices intended to conceal a defect in an elastic medium. In the former, a so-called cloak is severely biased toward one or a few specific elastic disturbances, whereas in the latter, an unbiased cloak is effective under any elastic disturbance. We propose a two-stage approach for optimization-based design of elastostatic cloaks that targets true, unbiased cloaks. First, we perform load-case...
• Activity and structure of human (d)CTP deaminase CDADC1 AI翻译
  作者：Slyvka, Anton; Rathore, Ishan; Yang, Renbin; Gewartowska, Olga; Kanai, Tapan; Lountos, George T.; Skowronek, Krzysztof; Czarnocki-Cieciura, Mariusz; Wlodawer, Alexander; Bochtler, Matthias
  作者单位：Miedzynarodowy Instytut Biologii Molekularnej i Komorkowej; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI); National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI); Frederick National Laboratory for Cancer Research; Miedzynarodowy Instytut Biologii Molekularnej i Komorkowej; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI); Frederick National Laboratory for Cancer Research; Miedzynarodowy Instytut Biologii Molekularnej i Komorkowej; Miedzynarodowy Instytut Biologii Molekularnej i Komorkowej; Polish Academy of Sciences; Institute of Biochemistry & Biophysics - Polish Academy of Sciences
  摘要：Vertebrates have evolved an understudied protein termed CDADC1 (NYD-SP15) that contains an inactive N-terminal and active C-terminal DCTD-like domain. Here, we show that human CDADC1 is a (d)CTP-specific deaminase, with a roughly 2-fold in vitro preference for dCTP over CTP. We determined high-resolution cryo-EM structures of CDADC1 in the absence of substrate and in complex with dCTP and 5-methyl-dCTP. The structures show that CDADC1 forms trimers and dimers of trimers in solution. The (d)CTP...
• Interferon- induced activation of dendritic cells and monocytes by yellow fever vaccination correlates with early antibody responses AI翻译
  作者：Winheim, Elena; Santos-Peral, Antonio; Ehm, Tamara; Rinke, Linus; Riemer, Sandra; Zaucha, Magdalena; Goresch, Sebastian; Lehmann, Lisa; Eisenaecher, Katharina; Pritsch, Michael; Barba-Spaeth, Giovanna; Straub, Tobias; Rothenfusser, Simon; Krug, Anne B.
  作者单位：University of Munich; University of Munich; Helmholtz Association; Helmholtz-Center Munich - German Research Center for Environmental Health; University of Munich; Centre National de la Recherche Scientifique (CNRS); CNRS - National Institute for Biology (INSB); Pasteur Network; Universite Paris Cite; Institut Pasteur Paris; University of Munich
  摘要：Yellow fever vaccination provides long- lasting protection and is a unique model for studying the immune response to an acute RNA virus infection in humans. To elucidate the early innate immune events preceding the rapid generation of protective immunity, we performed transcriptome analysis of human blood dendritic cell (DC) and monocyte subpopulations before and 3, 7, 14, and 28 d after vaccination. We detected temporary upregulation of IFN- stimulated genes (ISG) in all DC and monocyte subse...
• In silico machine learning-enabled detection of polycyclic aromatic hydrocarbons from contaminated soil AI翻译
  作者：Ju, Yilong; Neumann, Oara; Denison, Sara B.; Jin, Peixuan; Alvarado, Andres B. Sanchez-; Nordlander, Peter; Senftle, Thomas P.; Alvarez, Pedro J. J.; Patel, Ankit; Halas, Naomi J.
  作者单位：Rice University; Rice University; Rice University; Rice University; Rice University; Rice University; Rice University; Baylor College of Medicine
  摘要：The detection and identification of polycyclic aromatic hydrocarbons (PAHs) and their modified derivatives in contaminated soil is challenging due to the chemical and microbial complexity of soil organic matter. To address these challenges, we developed an innovative analytical approach that combines Surface-enhanced Raman spectroscopy with a Raman spectral library constructed in silico using density functional theory (DFT)-calculated spectra. This method overcomes several limitations associat...
• Cryptic genetic variation in brain gene expression precedes the evolution of cannibalism in spadefoot toad tadpoles AI翻译
  作者：Nesta, Dante J.
  作者单位：Indiana University System; Indiana University Bloomington
  摘要：The origins of novel behaviors are poorly understood, despite behavior's hypothesized roles in evolution. One model, genetic accommodation, proposes that selection on ancestral phenotypic plasticity may precede the evolution of novel traits. A critical assumption of genetic accommodation is that ancestral lineages possess heritable genetic variation for trait plasticity that is revealed in novel environments, thereby providing the raw materials for subsequent refinement of the novel trait in d...
• Paving the way for social touch at a distance: Sonifying tactile interactions and their underlying emotions AI翻译
  作者：de Lagarde, Alexandra; Pelachaud, Catherine; Kirsch, Louise P.; Auvray, Malika
  作者单位：Sorbonne Universite; Centre National de la Recherche Scientifique (CNRS); Universite Paris Cite; Centre National de la Recherche Scientifique (CNRS)
  摘要：Social touch is crucial for human well-being, as a lack of tactile interactions increases anxiety, loneliness, and need for social support. To address the detrimental effects of social isolation, we build on cutting-edge research on social touch and movement sonification to investigate whether social tactile gestures could be recognized through sounds, a sensory channel giving access to remote information. Four online experiments investigated participants' perception of auditory stimuli that w...