32期

最新文章

• Hide-and-Seek genome editing reveals that Gephyrin is required for axo-axonic synapse assembly AI翻译
  作者：Ogawa, Yuki; Nguyen, Duc V. M.; Ogawa, Ayano; Rasband, Matthew N.
  作者单位：Baylor College of Medicine
  摘要：The visualization and manipulation of proteins in neurons is widely used to deduce their functions. While every experimental approach has limitations, the concurrent knock-in and knockout of two different proteins can be especially challenging. To this end, we developed Hide-and-Seek genome editing, which allows the simultaneous visualization and knockout of proteins in neurons using Adeno-associated viral vectors and the CRISPR/Cas9 system. We demonstrate the efficacy and flexibility of this ...
• Toward a molecular logic of lipid droplet heterogeneity? AI翻译
  作者：Vanni, Stefano
  作者单位：University of Fribourg; University of Fribourg
• Phosphatidic acid production on the vacuole harboring Legionella pneumophila is a signal for recognition of interferon-induced GTPases AI翻译
  作者：Oide, Hiromu; Kubori, Tomoko; Nagai, Hiroki; Nozawa, Takashi; Nakagawa, Ichiro; Sasai, Miwa; Yamamoto, Masahiro; Arasaki, Kohei
  作者单位：Tokyo University of Pharmacy & Life Sciences; Gifu University; Gifu University; Kyoto University; University of Osaka; University of Osaka; University of Osaka
  摘要：The interferon (IFN)-inducible GTPases play a crucial role in cell autonomous immunity against intracellular pathogens. Particularly, these GTPases directly recognize the host membrane-derived vacuole containing pathogens and subsequently destroy it. Although it has been revealed that these GTPases target the membrane of Legionella pneumophila (L. pneumophila)-containing vacuole (LCV), molecular mechanism has been totally uncleared. Here, we show that mouse guanylate-binding protein 2 (mGBP2) ...
• Pervasive horizontal transfer of adeno-associated virus capsid genes AI翻译
  作者：Gifford, Robert J.
  作者单位：Stellenbosch University; University of Glasgow
  摘要：Adeno-associated viruses (AAVs) are nonpathogenic DNA viruses with potent gene delivery capabilities, making them essential tools in gene therapy and biomedical research. Despite their therapeutic importance, key aspects of AAV natural biology remain obscure, complicating efforts to explain rare AAV-associated diseases and optimize gene therapy vectors. By analyzing sequence data from virus isolates and endogenous viral elements (EVEs), I reveal a striking evolutionary pattern: While AAV subli...
• A 75,000-y-old Scandinavian Arctic cave deposit reveals past faunal diversity and paleoenvironment AI翻译
  作者：Walker, Samuel J.; Boilard, Aurelie; Henriksen, Mona; Lord, Edana; Robu, Marius; Buylaert, Jan - Pieter; Beijersbergen, Liselotte M. Takken; Halvorsen, Lene Synnove; Cintron, Adriana M.; Onshuus, Emma Katrin; Cockerill, Christopher Alan; Ujvari, Gabor; Palcsu, Laszlo; Temovski, Marjan; Maccali, Jenny; Linge, Henriette; Olsen, Jesper; Aksnes, Sverre; Bertheussen, Anastasia; Lygre, Ola; Alsos, Inger G.; Dalen, Love; Star, Bastiaan; Hufthammer, Anne Karin; van Kolfschoten, Thijs; Lauritzen, Stein- Erik; Lodoen, Trond Klungseth; Boessenkool, Sanne
  作者单位：University of Oslo; Bournemouth University; Norwegian University of Life Sciences; Swedish Museum of Natural History; Stockholm University; Emil Racovita Institute of Speleology; University of Bucharest; Technical University of Denmark; University of Bergen; HUN-REN; HUN-REN Research Centre for Astronomy & Earth Sciences; Institute for Geological & Geochemical Research - HAS; HUN-REN; HUN-REN Institute for Nuclear Research; University of Bergen; University of Bergen; Bjerknes Centre for Climate Research; University of Bergen; Aarhus University; University of Bergen; UiT The Arctic University of Tromso; Swedish Museum of Natural History; Leiden University; Leiden University - Excl LUMC; Shandong University
  摘要：During the last glacial period (similar to 118 to 11.7 ka), the Arctic has been characterized by a major redistribution of flora and fauna as a consequence of extreme climatic fluctuations, with associated glacial advances and retreats, sea-level changes, and shifting sea ice extent. In the high-latitude regions of Northern Europe that are currently subject to rapid climate warming, we lack a comprehensive understanding of faunal biodiversity in the last glacial period due to the extreme rarit...
• Yeast adapts to diverse ecological niches driven by genomics and metabolic reprogramming AI翻译
  作者：Wang, Haoyu; Nielsen, Jens; Zhou, Yongjin J.; Lu, Hongzhong
  作者单位：Shanghai Jiao Tong University; Chinese Academy of Sciences; Dalian Institute of Chemical Physics, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Chalmers University of Technology; Chinese Academy of Sciences; Dalian Institute of Chemical Physics, CAS; Chinese Academy of Sciences; Dalian Institute of Chemical Physics, CAS
  摘要：The famous model organism Saccharomyces cerevisiae is widely present in a variety of natural and human-associated habitats. Despite extensive studies of this organism, the metabolic mechanisms driving its adaptation to varying niches remain elusive. We here gathered genomic resources from 1,807 S. cerevisiae strains and assembled them into a high-quality pangenome, facilitating the comprehensive characterization of genetic diversity across isolates. Utilizing the pangenome, 1,807 strain-specif...
• Phase transfer-driven formation of solid metal-organic materials with tunable optical and adsorption properties AI翻译
  作者：Du, Jia- Xing; Liu, Lingran; Yu, Mei- Lin; Zhang, Fengmin; Stang, Peter J.; Sun, Yan
  作者单位：Henan University; Yangzhou University; Utah System of Higher Education; University of Utah
  摘要：Precise control of the physicochemical properties of metal-organic materials has been a challenge in the fields of materials science and engineering and can be achieved through the construction of morphology/size-dependent supramolecular functional model systems. The development of diverse hierarchical micro/nanostructured systems based on similar molecules and the elucidation of their morphology-dependent characteristics provide the possibility for efficiently constructing this system and stu...
• Schaumann and Alastrué de Asenjo, Weakening AMOC reduces ocean carbon uptake and increases the social cost of carbon ( vol 122 , e2419543122 , 2025 ) AI翻译
  作者：Schaumann, Felix; de Asenjo, Eduardo Alastrue
• A twisted chromophore powers a turn- on fluorescent protein chloride sensor AI翻译
  作者：Chen, Cheng; Pathiranage, Vishaka; Ong, Whitney S. Y.; Dodani, Sheel C.; Walker, Alice R.; Fang, Chong
  作者单位：Oregon State University; Wayne State University; University of Texas System; University of Texas Dallas
  摘要：Fluorescent proteins (FPs) are noninvasive genetically encodable probes that have revolutionized bioimaging and health fields with vivid images and an ever-growing repertoire from jellyfish to sea anemones and corals. Inside the protein matrix, chromophore non-planarity and flexibility have long been argued to govern the fluorescence efficiency of FPs, yet their fundamental roles and relative importance have been elusive which hinder the rational design of versatile FPs and biosensors. Herein,...
• Reinstating memories' temporal context at encoding causes Sisyphus-like memory rejuvenation AI翻译
  作者：Baeuml, Karl-Heinz T.; Meixensperger, Sarah R.; Hirsch, Marilena L.
  作者单位：University of Regensburg
  摘要：As memories age, their immediate retrievability decreases albeit, due to ongoing memory consolidation, their future rate of forgetting weakens. Here, we show in two experiments (N = 1.216 participants) that mentally traveling back in time to older memories' temporal context at encoding reverses the two effects and makes the memories similar again to how they were at an earlier point in time. Mental time travel increased both the memories' immediate retrievability and their future rate of forge...