32期

最新文章

• Cryptic intronic transcriptional initiation generates efficient endogenous mRNA templates for C9orf72-associated RAN translation AI翻译
  作者：Miller, Shannon L.; Green, Katelyn M.; Crone, Bradley; Switzenberg, Jessica A.; Tank, Elizabeth M. H.; Krans, Amy; Jansen-West, Karen; Wieland, Clare M.; Ji, Eric W.; Petrucelli, Leonard; Barmada, Sami J.; Boyle, Alan P.; Todd, Peter K.
  作者单位：University of Michigan System; University of Michigan; University of Michigan System; University of Michigan; University of Michigan System; University of Michigan; Mayo Clinic; University of Michigan System; University of Michigan; University of Michigan System; University of Michigan; University of Michigan System; University of Michigan
  摘要：Intronic GGGGCC hexanucleotide repeat expansions in C9orf72 are the most common genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Despite its intronic location, this repeat avidly supports synthesis of pathogenic dipeptide repeat (DPR) proteins via repeat-associated non-AUG (RAN) translation. However, the template RNA species that undergoes RAN translation endogenously remains unclear. Using long-read based 5 ' RNA ligase-mediated rapid amplification of cD...
• Logarithmic kinetics and bundling in random packings of elongated 3D physical links AI翻译
  作者：Bonamassa, Ivan; Rath, Balazs; Posfai, Marton; Abert, Miklos; Keliger, Daniel; Szegedy, Balazs; Kertesz, Janos; Lovasz, Laszlo; Barabasi, Albert-Laszlo
  作者单位：Budapest University of Technology & Economics; Northeastern University
  摘要：We explore the impact of excluded volume interactions on the local assembly of linear physical networks, where nodes are spheres and links are rigid cylinders with varying length. To focus on the effect of elongated links, we introduce a minimal 3D model that helps us zoom into confined regions of these networks whose distant parts are sequentially connected by the random deposition of physical links with a very large aspect ratio. We show that the nonequilibrium kinetics at which these elonga...
• Role of wettability, adhesion, and instabilities in transitions during lubricated sliding friction AI翻译
  作者：Dong, Hao; Siddiquie, Reshma Y.; Xiao, Xuemei; Andrews, Michael; Bergman, Brian; Hui, Chung-Yuen; Jagota, Anand
  作者单位：Lehigh University; Lehigh University; Cornell University; Michelin
  摘要：Lubricated contacts in soft materials are important in various engineering systems and natural settings. Three major lubrication regimes are boundary (BL), mixed (ML), and elastohydrodynamic (EHL) lubrication, where the contact region is dry, partially wetted, or fully wetted, respectively. The transition between these regimes is insufficiently understood, especially for soft contacts, which impedes desired control of lubricated sliding friction. Here, we report on the role of solid wettabilit...
• The entities enabling scientific fraud at scale are large, resilient, and growing rapidly AI翻译
  作者：Richardson, Reese A. K.; Hong, Spencer S.; Byrne, Jennifer A.; Stoeger, Thomas; Amaral, Luis A. Nunes
  作者单位：Northwestern University; Northwestern University; Northwestern University; University of Sydney; Northwestern University; Northwestern University; Northwestern University; Feinberg School of Medicine; Northwestern University; Northwestern University
  摘要：Science is characterized by collaboration and cooperation, but also by uncertainty, competition, and inequality. While there has always been some concern that these pressures may compel some to defect from the scientific research ethos-i.e., fail to make genuine contributions to the production of knowledge or to the training of an expert workforce-the focus has largely been on the actions of lone individuals. Recently, however, reports of coordinated scientific fraud activities have increased....
• Confined colloidal droplets dry to form circular mazes AI翻译
  作者：Beechey-Newman, Ilaria; Kizilova, Natalya; Hennig, Andreas Andersen; Flekkoy, Eirik Crude; Eiser, Erika
  作者单位：Norwegian University of Science & Technology (NTNU); Warsaw University of Technology; University of Oslo; Norwegian University of Science & Technology (NTNU); University of Cambridge
  摘要：During drying, particle-laden sessile droplets will leave so-called coffee-stain rings behind. This phenomenon is well known and well understood (Deegan et al., Nature 389, 827-829 (1997). Here, we show that when particle-laden droplets confined in a slit are allowed to evaporate very slowly, they do not deposit coffee rings, but form surprisingly intricate, circular maze-like patterns. We present experiments that illustrate this pattern formation and discuss the factors that determine when su...
• Exploring glycoform- dependent dynamic modulations in human immunoglobulin G via computational and experimental approaches AI翻译
  作者：Yanaka, Saeko; Sakae, Yoshitake; Miyanoiri, Yohei; Yamaguchi, Takumi; Isono, Yukiko; Kondo, Sachiko; Iwasaki, Miyuki; Onitsuka, Masayoshi; Yagi, Hirokazu; Kato, Koichi
  作者单位：National Institutes of Natural Sciences (NINS) - Japan; The Exploratory Research Center on Life & Living Systems (ExCELLS); National Institutes of Natural Sciences (NINS) - Japan; Research Organization for Information Science & Technology (RIST); University of Osaka; Japan Advanced Institute of Science & Technology (JAIST); Nagoya City University; Tokushima University
  摘要：We investigate the impact of glycoform alterations on the dynamic structure of the human immunoglobulin G1 (IgG1) Fc region using integrated computational and experimental approaches. Four distinct IgG1-Fc glycoforms, varying in core fucosylation and nonreducing terminal galactosylation, were generated through a combination of cell engineering and in vitro enzymatic reactions. Stable-isotope-assisted NMR spectroscopy, incorporating both glycan and protein signals, revealed that galactosylation...
• Intranasal measles virus-and mumps virus-based SARS-CoV-2 vaccine candidates prevent SARS-CoV-2 infection and transmission AI翻译
  作者：Hsu, Cheng Chih; Chamblee, Michelle; Ye, Chengjin; Shamseldin, Mohamed M.; Yoo, Sung J.; Li, Pei; Zhang, Yuexiu; Liu, Yajie; Hall, Jesse M.; Xu, Jiayu; Miao, Hanson; Thongpan, Ilada; Mahesh, K. C.; Liang, Xueya; Yount, Jacob S.; Peeples, Mark E.; Boyaka, Prosper N.; Dubey, Purnima; Martinez-Sobrido, Luis; Liu, Shan-Lu; Li, Jianrong
  作者单位：University System of Ohio; Ohio State University; Texas Biomedical Research Institute; University System of Ohio; Ohio State University; Egyptian Knowledge Bank (EKB); Capital University - Egypt; University System of Ohio; Ohio State University; Nationwide Childrens Hospital; University System of Ohio; Ohio State University; University System of Ohio; Ohio State University
  摘要：The emergence of immune-evasive SARS-CoV-2 Omicron subvariants highlights the need to develop a mucosal SARS-CoV-2 vaccine that can provide broad protection against virus infection and transmission. Here, we developed an intranasal monovalent SARS-CoV-2 vaccine expressing the six-proline-stabilized prefusion spike proteins (preS-6P) of Omicron XBB.1.5 based on the attenuated mumps virus (MuV) Jeryl Lynn (JL1) vaccine strain. We also developed an intranasal trivalent vaccine expressing the preS...
• Some topological genera and Jacobi forms AI翻译
  作者：Amdeberhan, Tewodros; Griffin, Michael J.; Ono, Ken
  作者单位：Tulane University; Vanderbilt University; University of Virginia
  摘要：We revisit and elucidate the A-genus, Hirzebruch's L-genus, and Witten's W-genus, cobordism invariants of special classes of manifolds. After slight modification, involving Hecke's trick, we find that the A-genus and L-genus arise directly from Jacobi's theta function. For every k >= 0, we obtain exact formulas for the quasimodular expressions of A(k) and L-k as traces of partition Eisenstein series A(K)(tau ) = Tr-k(Phi(A):tau) and L-k(tau) = Tr-k (Phi(L):tau) which are easily converted to th...
• Transcription termination promotes splicing efficiency and fidelity in a compact genome AI翻译
  作者：Barr, Keaton; He, Kevin L.; Krumbein, Andreas J.; Chanfreau, Guillaume F.
  作者单位：University of California System; University of California Los Angeles; University of California System; University of California Los Angeles
  摘要：Splicing of terminal introns is coupled to 3 '-end processing by cleavage and polyadenylation (CPA) in mammalian genes. Whether this functional coupling is universally conserved across eukaryotes is unclear. Here, we show using long read RNA sequencing in Saccharomyces cerevisiae that splicing inactivation does not result in widespread CPA impairment, and that inactivation of CPA has limited impact on splicing efficiency. The negative impact of CPA inactivation on splicing is mainly due to tra...
• Tracing SARS-CoV-2 clusters across local scales using genomic data AI翻译
  作者：Lyu, Leke; Gill, Mandev; Stott, Guppy; Subedi, Sachin; Dailey, Cody; Veytsel, Gabriella; Alabady, Magdy; Fujimoto, Kayo; Penn, Ryker; Brown, Pamela; Sealy, Roger; Bahl, Justin
  作者单位：University System of Georgia; University of Georgia; University System of Georgia; University of Georgia; University System of Georgia; University of Georgia; University System of Georgia; University of Georgia; University System of Georgia; University of Georgia; University System of Georgia; University of Georgia; University of Texas System; University of Texas Health Science Center Houston
  摘要：A quantitative understanding of local transmission dynamics is essential for designing effective prevention strategies. In this study, we developed a computational workflow to identify viral introductions and trace locally circulating clusters. We analyzed over 26,000 SARS-CoV-2 genomes and their associated metadata, collected between January and October 2021, to explore introduction and local dispersal patterns in Greater Houston, a major metropolitan area known for its demographic diversity....