2025

最新文章

• Thermodynamics of calcium binding to heparin: Implications of solvation and water structuring for polysaccharide biofunctions AI翻译
  作者：Knight, Brenna M.; Gallagher, Connor M. B.; Schulz, Michael D.; Edgar, Kevin J.; McNaul, Caylyn D.; McCutchin, Christina A.; Dove, Patricia M.
  作者单位：Virginia Polytechnic Institute & State University; Virginia Polytechnic Institute & State University; Virginia Polytechnic Institute & State University; Virginia Polytechnic Institute & State University; Virginia Polytechnic Institute & State University; Virginia Polytechnic Institute & State University
  摘要：Heparan sulfates are found in all animal tissues and have essential roles in living systems. This family of biomacromolecules modulates binding to calcium ions (Ca2+) in low free energy reactions that influence biochemical processes from cell signaling and anticoagulant efficacy to biomineralization. Despite their ubiquity, the thermodynamic basis for how heparans and similarly functionalized biomolecules regulate Ca2+ interactions is not yet established. Using heparosan (Control) and heparins...
• Reconfigurable photothermal doping filament for selective spin manipulation and addressing AI翻译
  作者：Liu, Zhi-Wei; Ma, Meng-Qi; Sun, Bo-Wen; Li, Liang; Jiang, Wang; Zang, Han-Xiang; Bai, Zhe; Dong, Yang; Zhang, Shao-Chun; Chen, Xiang-Dong; Zou, Chong-Wen; Guo, Guang-Can; Sun, Fang-Wen
  作者单位：Chinese Academy of Sciences; University of Science & Technology of China, CAS; Chinese Academy of Sciences; University of Science & Technology of China, CAS; CAS Center for Excellence in Quantum Information & Quantum Physics; Chinese Academy of Sciences; University of Science & Technology of China, CAS; Hefei National Laboratory; Chinese Academy of Sciences; University of Science & Technology of China, CAS
  摘要：The room temperature manipulation of solid-state spins provides an opportunity to develop quantum applications under ambient conditions. Local electromagnetic fields, that usually produced by current in micro/nanoscale metal wires, have been employed for the coherent driving and addressing of spin qubit. However, the fixed distribution limits the spatial selectivity and efficiency of qubit manipulation, which is of central importance in a scaled-up quantum system. Here, we report a solution by...
• CRISPR with Transcriptional Readout reveals influenza transcription is modulated by NELF and can precipitate an interferon response AI翻译
  作者：Vicary, Alison C.; Jordan, Sydney N. Z.; Mendes, Marisa; Swaminath, Sharmada; Castro, Lennice K.; Porter, Justin S.; Vo, Kevin D.; Russell, Alistair B.
  作者单位：University of California System; University of California San Diego
  摘要：Transcription of interferons upon viral infection is critical for cell-intrinsic innate immunity. This process is influenced by many host and viral factors. To identify host factors that modulate interferon induction within cells infected by influenza A virus, we developed CRISPR with Transcriptional Readout using sequencing (CRITR-seq). CRITR-seq is a method linking CRISPR guide sequence to activity at a promoter of interest. Employing this method, we find that depletion of the Negative Elong...
• FADDDED filaments coordinate complex IIa assembly during TNF-induced apoptosis AI翻译
  作者：Chen, Ying; Huynh, Vinh Thang; Lai, Lihua; Liu, Ping; Li, Tongyang; Tan, Yaw Bia; Chew, Che Shin; Velazquez, Amhed Missael Vargas; Samsudin, Firdaus; Marzinek, Jan K.; Bond, Peter J.; Wu, Bin; Luo, Dahai; Tergaonkar, Vinay
  作者单位：Agency for Science Technology & Research (A*STAR); A*STAR - Institute of Molecular & Cell Biology (IMCB); Nanyang Technological University; Nanyang Technological University; Agency for Science Technology & Research (A*STAR); A*STAR - Bioinformatics Institute (BII); National University of Singapore; Nanyang Technological University; National Centre for Infectious Diseases Singapore; National University of Singapore
  摘要：Extrinsic apoptosis is initiated by signaling from death receptors, leading to the assembly of RIPK1, FADD, and caspase-8 complex. Subsequently, caspase-8 forms a filamentous structure through the oligomerization of its tandem death effector domain (tDED), resulting in caspase activation and cell death. Although the DED of FADD (FADDDED) is homologous to the tDEDs of caspase-8 (casp8tDED) and both oligomerize to function, the functional form of FADDDED oligomer in extrinsic apoptosis remains u...
• Into the belly of the brainstem: The neurons of the extended enteric nervous system AI翻译
  作者：Moore, Jeffrey D.
  作者单位：University of Southern California
• A critical role for Vibrio parahaemolyticus LPS to mediate evasion of host immune response during infection AI翻译
  作者：Jaishankar, Jananee; Yang, Hyojik; O'Keefe, Ian P.; Tenaglia, Brandon M.; Kinch, Lisa N.; Ernst, Robert K.; Orth, Kim
  作者单位：University of Texas System; University of Texas Southwestern Medical Center; Howard Hughes Medical Institute; University System of Maryland; University of Maryland Baltimore; University System of Maryland; University of Maryland Baltimore; University of Texas System; University of Texas Southwestern Medical Center
  摘要：Vibrio parahaemolyticus (V. para), a Gram-negative halophilic bacterium, is the leading cause of seafood-borne gastroenteritis. While the cytotoxic and enterotoxic capabilities of this bacterium have been previously investigated, the strategies it employs to circumvent innate immune detection by the host remain poorly understood. Bacterial lipopolysaccharide (LPS) serves as the major pathogen-associated molecular pattern that triggers host inflammatory responses, and its lipid A structure cont...
• Keys to the global treeline formation: Thermal limit for its position and moisture for the taxon-specific variation AI翻译
  作者：Xie, Yuyang; Shen, Zehao; Camarero, J. Julio; Penuelas, Josep; Wang, Xuejing; Li, Jitang; Rao, Wenge; Chen, Xiangwu; Zhao, Fu; Feng, Xiao
  作者单位：Peking University; Peking University; University of North Carolina; University of North Carolina Chapel Hill; University of North Carolina School of Medicine; Yunnan University; Consejo Superior de Investigaciones Cientificas (CSIC); CSIC - Instituto Pirenaico de Ecologia (IPE); Centro de Investigacion Ecologica y Aplicaciones Forestales (CREAF-CERCA); Autonomous University of Barcelona; Centro de Investigacion Ecologica y Aplicaciones Forestales (CREAF-CERCA)
  摘要：Alpine treeline is a prominent biogeographic feature worldwide, determined by the physiological limit of tree life form. There are considerable variations in the various dimensions of physiological limit among tree taxa; thus, varied environmental drivers and spatial patterns are expected for different tree taxa at treelines. However, such taxonomic variability of treeline is often overlooked in large-scale studies. Here, we assembled, to our knowledge, the most comprehensive dataset of tree s...
• Keldysh tuning of photoluminescence in a lead halide perovskite crystal AI翻译
  作者：Zhang, Zhuquan; Ning, Honglie; Liu, Zi-Jie; Hou, Jin; Mohite, Aditya D.; Baldini, Edoardo; Gedik, Nuh; Nelson, Keith A.
  作者单位：Massachusetts Institute of Technology (MIT); Massachusetts Institute of Technology (MIT); Rice University; Rice University; University of Texas System; University of Texas Austin
  摘要：In 1964, Keldysh laid the groundwork for strong-field physics in atomic, molecular, and solid-state systems by delineating a ubiquitous transition from multiphoton absorption to classical field-driven electron tunneling under intense electromagnetic waves. While both processes in semiconductors can generate carriers and result photon emission through electron-hole recombination, the low quantum yields most materials have hindered direct observation of the Keldysh crossover. Leveraging the larg...
• Confined colloidal droplets dry to form circular mazes AI翻译
  作者：Beechey-Newman, Ilaria; Kizilova, Natalya; Hennig, Andreas Andersen; Flekkoy, Eirik Crude; Eiser, Erika
  作者单位：Norwegian University of Science & Technology (NTNU); Warsaw University of Technology; University of Oslo; Norwegian University of Science & Technology (NTNU); University of Cambridge
  摘要：During drying, particle-laden sessile droplets will leave so-called coffee-stain rings behind. This phenomenon is well known and well understood (Deegan et al., Nature 389, 827-829 (1997). Here, we show that when particle-laden droplets confined in a slit are allowed to evaporate very slowly, they do not deposit coffee rings, but form surprisingly intricate, circular maze-like patterns. We present experiments that illustrate this pattern formation and discuss the factors that determine when su...
• Exploring glycoform- dependent dynamic modulations in human immunoglobulin G via computational and experimental approaches AI翻译
  作者：Yanaka, Saeko; Sakae, Yoshitake; Miyanoiri, Yohei; Yamaguchi, Takumi; Isono, Yukiko; Kondo, Sachiko; Iwasaki, Miyuki; Onitsuka, Masayoshi; Yagi, Hirokazu; Kato, Koichi
  作者单位：National Institutes of Natural Sciences (NINS) - Japan; The Exploratory Research Center on Life & Living Systems (ExCELLS); National Institutes of Natural Sciences (NINS) - Japan; Research Organization for Information Science & Technology (RIST); University of Osaka; Japan Advanced Institute of Science & Technology (JAIST); Nagoya City University; Tokushima University
  摘要：We investigate the impact of glycoform alterations on the dynamic structure of the human immunoglobulin G1 (IgG1) Fc region using integrated computational and experimental approaches. Four distinct IgG1-Fc glycoforms, varying in core fucosylation and nonreducing terminal galactosylation, were generated through a combination of cell engineering and in vitro enzymatic reactions. Stable-isotope-assisted NMR spectroscopy, incorporating both glycan and protein signals, revealed that galactosylation...