2025

最新文章

• Volumetric imaging of the 3D orientation of cellular structures with a polarized fluorescence light-sheet microscope AI翻译
  作者：Chandler, Talon; Guo, Min; Su, Yijun; Chen, Jiji; Wu, Yicong; Liu, Junyu; Agashe, Atharva; Fischer, Robert S.; Mehta, Shalin B.; Kumar, Abhishek; Baskin, Tobias I.; Jaumouille, Valentin; Liu, Huafeng; Swaminathan, Vinay; Nain, Amrinder S.; Oldenbourg, Rudolf; La Riviere, Patrick J.; Shroff, Hari
  作者单位：Chan Zuckerberg Initiative (CZI); University of Chicago; Zhejiang University; National Institutes of Health (NIH) - USA; NIH National Institute of Biomedical Imaging & Bioengineering (NIBIB); National Institutes of Health (NIH) - USA; Howard Hughes Medical Institute; Virginia Polytechnic Institute & State University; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI); Marine Biological Laboratory - Woods Hole; University of Massachusetts System; University of Massachusetts Amherst; Marine Biological Laboratory - Woods Hole; Simon Fraser University; Scania; Lund University; Lund University; Scania
  摘要：Polarized fluorescence microscopy is a valuable tool for measuring molecular orientations in biological samples, but techniques for recovering three-dimensional orientations and positions of fluorescent ensembles are limited. We report a polarized dual-view light-sheet system for determining the diffraction-limited three-dimensional distribution of the orientations and positions of ensembles of fluorescent dipoles that label biological structures. We share a set of visualization, histogram, an...
• Recurrent DNA nicks drive massive expansions of (GAA)n repeats (vol 121, e2413298121, 2024) AI翻译
  作者：Li, Liangzi; Scott, W. Shem; Khristich, Alexandra N.; Armenia, Jillian F.; Mirkin, Sergei M.
• Computational and in vitro evaluation of probiotic treatments for nasal Staphylococcus aureus decolonization AI翻译
  作者：Tepekule, Burcu; Barcik, Weronika; Staiger, Willy I.; Bergada-Pijuan, Judith; Scheier, Thomas; Brulisauer, Laura; Hall, Alex R.; Gunthard, Huldrych F.; Hilty, Markus; Kouyos, Roger D.; Brugger, Silvio D.
  作者单位：University of Zurich; University Zurich Hospital; University of Zurich; University Zurich Hospital; University of Zurich; Swiss Federal Institutes of Technology Domain; ETH Zurich; University of Bern
  摘要：Despite the rising challenge of antibiotic resistance, current approaches to eradicate nasal pathobionts Staphylococcus aureus and Streptococcus pneumoniae rely on antibac-terials. An alternative is the artificial inoculation of commensal bacteria, i.e., probiotic treatment, supported by the increasing evidence for commensal- mediated inhibition of pathogens. To systematically investigate the potential of this approach, we developed a quantitative framework simulating the nasal microbiome dyna...
• Profile of Christopher B. Barrett AI翻译
  作者：Viegas, Jennifer
• OsNLP3 and OsPHR2 orchestrate direct and mycorrhizal pathways for nitrate uptake by regulating NAR2.1-NRT2s complexes in rice AI翻译
  作者：Wang, Shuangshuang; Ye, Hanghang; Yang, Congfan; Zhang, Yan; Pu, Jiawen; Ren, Yuhan; Xie, Kun; Wang, Lingxiao; Zeng, Dechao; He, Haoqiang; Ji, Haoyan; Herrera-Estrella, Luis Rafael; Xu, Guohua; Chen, Aiqun
  作者单位：Nanjing Agricultural University; Texas Tech University System; Texas Tech University; Instituto Politecnico Nacional - Mexico; CINVESTAV - Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional; Nanjing Agricultural University
  摘要：Nitrogen (N) is the most important essential nutrient required by plants. Most land plants have evolved two N uptake pathways, a direct root pathway and a symbiotic pathway, via association with arbuscular mycorrhizal (AM) fungi. However, the interaction between the two pathways is ambiguous. Here, we report that OsNAR2.1-OsNRT2s, the nitrate (NO3-) transporter complexes with crucial roles in direct NO3 - uptake, are also recruited for symbiotic NO3- uptake. OsNAR2.1 and OsNRT2.1/2.2 are coreg...
• Observed declines in upper ocean phosphate- to- nitrate availability AI翻译
  作者：Gerace, Skylar D.; Yu, Jun; Moore, J. Keith; Martiny, Adam C.
  作者单位：University of California System; University of California Irvine; University of California System; University of California Irvine
  摘要：Climate warming is increasing ocean stratification, which in turn should decrease the nutrient flux to the upper ocean. This may slow marine primary productivity, causing cascading effects throughout food webs. However, observing changes in upper ocean nutrients is challenging because surface concentrations are often below detection limits. We show that the nutricline depth, where nutrient concentrations reach well- detected levels, is tied to productivity and upper ocean nutrient availability...
• Abscisic acid signaling gates salt- induced responses of plant roots AI翻译
  作者：Lamers, Jasper; Zhang, Yanxia; van Zelm, Eva; Leong, Cheuk Ka; Meyer, A. Jessica; de Zeeuw, Thijs; Verstappen, Francel; Veen, Mark; Deolu-Ajayi, Ayodeji O.; Gommers, Charlotte M. M.; Testerink, Christa
  作者单位：Wageningen University & Research; Wageningen University & Research; South China Agricultural University; Utrecht University; Wageningen University & Research
  摘要：Soil salinity presents a dual challenge for plants, involving both osmotic and ionic stress. In response, plants deploy distinct yet interconnected mechanisms to cope with these facets of salinity stress. In this investigation, we observed a substantial overlap in the salt (NaCl)- induced transcriptional responses of Arabidopsis roots with those triggered by osmotic stress or the plant stress hormone abscisic acid (ABA), as anticipated. Notably, a specific cluster of genes responded uniquely t...
• The Regulatory- associated protein of target of rapamycin 1B (RAPTOR 1B) interconnects with the photoperiod pathway to promote flowering in Arabidopsis AI翻译
  作者：Urrea- Castellanos, Reynel; Calderan- Rodrigues, Maria J.; Artins, Anthony; Musialak- Lange, Magdalena; Macharanda- Ganesn, Appanna; Fernie, Alisdair R.; Wahl, Vanessa; Caldana, Camila
  作者单位：Max Planck Society; James Hutton Institute; Universidade de Sao Paulo
  摘要：The transition from vegetative to reproductive growth, or floral transition, is a tightly regulated, energy- demanding process. In Arabidopsis, the interplay of light perception and circadian rhythms detects changes in photoperiod length, accelerating flowering under long days (LD). CONSTANS (CO), a transcription factor, upregulates FLOWERING LOCUS T (FT) in leaves during dusk. The FT protein then moves to the shoot apical mer-istem, triggering the floral transition. While light and circadian ...
• Spatial propagation of temperate phages within and among biofilms AI翻译
  作者：Winans, James B.; Zeng, Lanying; Nadell, Carey D.
  作者单位：Dartmouth College; Dartmouth College; Texas A&M University System; Texas A&M University College Station
  摘要：Bacteria form groups composed of cells and a secreted polymeric matrix that controls their spatial organization. These groups-termed biofilms-can act as refuges from environmental disturbances and from biotic threats, including phages. Despite the ubiquity of temperate phages and bacterial biofilms, live propagation of temperate phages within biofilms has not been characterized on cellular spatial scales. Here, we leverage several approaches to track temperate phages and distinguish between ly...
• Endogenous LRRK2 and PINK1 function in a convergent neuroprotective ciliogenesis pathway in the brain AI翻译
  作者：Bagnoli, Enrico; Lin, Yu- En; Burel, Sophie; Jaimon, Ebsy; Antico, Odetta; Themistokleous, Christos; Nikoloff, Jonas M.; Squires, Samuel; Morella, Ilaria; Watzlawik, Jens O.; Fiesel, Fabienne C.; Springer, Wolfdieter; Tonelli, Francesca; Lis, Pawel; Brooks, Simon P.; Dunnett, Stephen B.; Brambilla, Riccardo; Alessi, Dario R.; Pfeffer, Suzanne R.; Muqit, Miratul M. K.
  作者单位：University of Dundee; Stanford University; University of Pavia; Cardiff University; Mayo Clinic; Mayo Clinic; Cardiff University
  摘要：Mutations in Leucine- rich repeat kinase 2 (LRRK2) and PTEN- induced kinase 1 (PINK1) are associated with familial Parkinson's disease (PD). LRRK2 phosphorylates Rab guanosine triphosphatase (GTPases) within the Switch II domain while PINK1 directly phosphorylates Parkin and ubiquitin (Ub) and indirectly induces phosphorylation of a subset of Rab GTPases. Herein we have crossed LRRK2 [R1441C] mutant knock- in mice with PINK1 knock- out (KO) mice and report that loss of PINK1 does not impact en...