OsNLP3 and OsPHR2 orchestrate direct and mycorrhizal pathways for nitrate uptake by regulating NAR2.1-NRT2s complexes in rice

• 成果类型: Article
• 署名作者: Wang, Shuangshuang; Ye, Hanghang; Yang, Congfan; Zhang, Yan; Pu, Jiawen; Ren, Yuhan; Xie, Kun; Wang, Lingxiao; Zeng, Dechao; He, Haoqiang; Ji, Haoyan; Herrera-Estrella, Luis Rafael; Xu, Guohua; Chen, Aiqun
• 署名单位: Nanjing Agricultural University; Texas Tech University System; Texas Tech University; Instituto Politecnico Nacional - Mexico; CINVESTAV - Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional; Nanjing Agricultural University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-14812
• DOI: 10.1073/pnas.2416345122
• 发表日期: 2025-02-18
• 关键词: arbuscular mycorrhiza; ammonium transporters; transcription factor; nitrogen; identification; expression; starvation; nutrition; symbioses; responses

摘要

Nitrogen (N) is the most important essential nutrient required by plants. Most land plants have evolved two N uptake pathways, a direct root pathway and a symbiotic pathway, via association with arbuscular mycorrhizal (AM) fungi. However, the interaction between the two pathways is ambiguous. Here, we report that OsNAR2.1-OsNRT2s, the nitrate (NO3-) transporter complexes with crucial roles in direct NO3 - uptake, are also recruited for symbiotic NO3- uptake. OsNAR2.1 and OsNRT2.1/2.2 are coregulated by NIN-like protein 3 (OsNLP3), a key regulator in NO3- signaling, and OsPHR2, a major regulator of phosphate starvation responses. More importantly, AM symbiosis induces expression of OsNAR2.1-OsNRT2s, OsNLP3, and OsSPX4, encoding an intracellular Pi sensor, in arbuscular- containing cells, but weakens their expression in the epidermis. OsNAR2.1 and OsNLP3 can activate both mycorrhizal NO3 - uptake and mycorrhization efficiency. Overall, we demonstrate that OsNLP3 and OsPHR2 orchestrate the direct and mycorrhizal NO3- uptake pathways by regulating the NAR2.1-NRT2s complexes in rice.