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作者：Ling, Jing; Liu, Yitong; Dumoulin, Alexandre; Sheng, Danli; Fu, Yunzhe; Liu, Shuzhang; Ding, Ling; Huang, Lulu; Xi, Peng; Tang, Hongyun; Stoeckli, Esther T.; Chen, Zhixing
作者单位：Peking University; Peking University; University of Zurich; University of Zurich; Westlake University; Peking University; Peking University; Peking University; Peking University; University of Zurich; Chinese Academy of Sciences; Peking University
摘要：Plasma membrane (PM) stains are important organelle markers for monitoring membrane morphology and dynamics. The state-of-the-art PM stains are bright, specific, fluorogenic, and compatible with superresolution imaging. However, when recording membrane dynamics using advanced fluorescence microscopes, PM is prone to photo-dynamic damage introduced by dyes due to its phospholipid bilayer nature. Here, we introduce PK Mem dyes tailored for time-lapse fluorescence imaging. By integrating triplet-...
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作者：van Egmond, Daisy M.; Lygeroudi, Anita; Kooij, Gijs
作者单位：Vrije Universiteit Amsterdam
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作者：Mantua, Nathan J.; Bell, Heather; Todgham, Anne E.; Daniels, Miles E.; Rinchard, Jacques; Ludwig, Jarrod M.; Field, John C.; Lindley, Steven T.; Rowland, Freya E.; Richter, Catherine A.; Walters, David; Finney, Bruce; Distajo, Haskell Anne R.; Tillitt, Donald; Honeyfield, Dale C.; Lipscomb, Taylor; Kwak, Kevin; Kindopp, Jason; Cocherell, Dennis; Ward, Abigail; Williams, Thomas H.; Harding, Jeff; Fangue, Nann A.; Jeffres, Carson; Ruiz-Cooley, Rocio I.; Litvin, Steven Y.; Foott, Scott; Adkison, Mark; Kormos, Brett; Harte, Peggy; Colwell, Frederick; Suffridge, Christopher P.; Shannon, Kelly C.; Cranford, Amanda; Ambrose, Charlotte; Reed, Aimee; Johnson, Rachel C.
作者单位：National Oceanic Atmospheric Admin (NOAA) - USA; University of California System; University of California Davis; University of California System; University of California Davis; University of California System; University of California Santa Cruz; State University of New York (SUNY) System; State University of New York (SUNY) Brockport; United States Department of the Interior; United States Geological Survey; Idaho State University; Idaho State University; United States Department of the Interior; United States Geological Survey; United States Department of the Interior; US Fish & Wildlife Service; University of California System; University of California Davis; Moss Landing Marine Laboratories; Monterey Bay Aquarium Research Institute; University of California System; University of California Davis; Oregon State University
摘要：Thiamine (vitamin B1) deficiency in marine systems is a globally significant threat to marine life. In 2020, newly hatched Chinook salmon (Oncorhynchus tshawytscha) fry in California's Central Valley (CCV) hatcheries swam in corkscrew patterns and died at unusually high rates due to a lack of this essential vitamin. We subsequently investigated the impacts and causes of thiamine deficiency in California's anadromous salmonids. Our laboratory studies defined the relationship between thiamine co...
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作者：Ngoi, Peter; Wang, Xianxi; Putta, Sivasankar; Da Luz, Ricardo F.; Serrao, Vitor Hugo B.; Emanuele, Michael J.; Rubin, Seth M.
作者单位：University of California System; University of California Santa Cruz; University of North Carolina; University of North Carolina Chapel Hill; University of North Carolina; University of North Carolina Chapel Hill; University of California System; University of California Santa Cruz
摘要：Cyclin F, a noncanonical member of the cyclin protein family, plays a critical role in regulating transitions in the cell division cycle. Unlike canonical cyclins, which bind and activate cyclin-dependent kinases (CDKs), Cyclin F functions as a substrate receptor protein within the Skp1-Cullin-F-box E3 ubiquitin ligase complex, enabling the ubiquitylation of target proteins. The structural features that distinguish Cyclin F as a ligase adaptor and the mechanisms underlying its selective substr...
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作者：Wood, Nicholas A.; Kraft, Alyssa; Shin, Kyungsoo; Gopinath, Tata; Marassi, Francesca M.
作者单位：Medical College of Wisconsin
摘要：The outer membrane vesicles (OMVs) produced by diderm bacteria have important roles in cell envelope homeostasis, secretion, interbacterial communication, and pathogenesis. The facultative intracellular pathogen Salmonella enterica Typhimurium (STm) activates OMV biogenesis inside the acidic vacuoles of host cells by upregulating the expression of the OM protein PagC, one of the most robustly activated genes in a host environment. Here, we used solid- state nuclear magnetic resonance (NMR) and...
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作者：Barrera, Francisco N.
作者单位：University of Tennessee System; University of Tennessee Knoxville
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作者：Borovska, Petra; de Haas, Benjamin
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作者：Garcia, Julian; Traulsen, Arne
作者单位：Monash University; Max Planck Society
摘要：Evolutionary game theory (EGT) has been pivotal in the study of cooperation, offering formal models that account for how cooperation may arise in groups of selfish, but simple agents. This is done by inspecting the complex dynamics arising from simple interactions between a few strategies in a large population. As such, the strategies at stake are typically hand-picked by the modeler, resulting in a system with many more individuals in the population than strategies available to them. In the p...
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作者：Liu, Xingyu; Yi, Li; Lin, Zongtao; Chen, Siyu; Wang, Shunyang; Sheng, Ying; Lebrilla, Carlito B.; Garcia, Benjamin A.; Xie, Yixuan
作者单位：Fudan University; Fudan University; Washington University (WUSTL); University of California System; University of California Davis; University of California System; University of California Davis
摘要：Protein-protein interactions (PPIs) are crucial for comprehending the molecular mechanisms and signaling pathways underlying diverse biological processes and disease progression. However, investigating PPIs involving membrane proteins is challenging due to the complexity and heterogeneity of glycosylation. To tackle this challenge, we developed an approach termed glycan-dependent affinity purification coupled with mass spectrometry (GAP-MS), specifically designed to characterize changes in gly...
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作者：Mann, Adam
