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作者：Cypranowska, Caroline A.; Feldthouse, Maya; Choi, Yoon Gi; Bakshinska, Dariya; Li, Rachel; Newman, Zachary L.; Isacoff, Ehud Y.
作者单位：University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of California System; University of California Berkeley; United States Department of Energy (DOE); Lawrence Berkeley National Laboratory; Francis Crick Institute; University of California System; University of California San Diego; University of Minnesota System; University of Minnesota Twin Cities
摘要：Weakening of synaptic transmission at the Drosophila larval neuromuscular junction triggers two forms of homeostatic compensation, one that increases the probability of glutamate release per action potential (Pr) and another that increases motoneuron (MN) activity. We investigated the molecular changes in MNs that underlie the increase in MN activity. RNA sequencing (RNA-seq) analysis on MNs whose glutamate release is weakened by knockdown of components of the MN transmitter release machinery ...
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作者：Dvir, Gony; Xing, Zenan; Beldman, Irina; Rivera, Andres; Wheeldon, Ian; Cutler, Sean R.
作者单位：University of California System; University of California Riverside; University of California System; University of California Riverside; University of California System; University of California Riverside; Universidad Nacional Autonoma de Mexico; University of California System; University of California Riverside; University of California System; University of California Riverside
摘要：Methods for fast and inexpensive gene synthesis from oligonucleotide pools enable rapid iteration of genetic designs. Here, we describe iggypop (indexed Golden Gate gene assembly from PCR- amplified oligonucleotide pools), a simple computational-experimental pipeline that allows for low- cost design and synthesis of hundreds of genes from oligonucleotide pools using Golden Gate assembly methods. We used iggypop to synthesize a series of single- transcript autonomously bioluminescent reporters ...
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作者：Hoskova, Michaela; Kotov, Oleg, V; Kucukoz, Betul; Murphyc, Catherine J.; Shegaia, Timur O.
作者单位：Chalmers University of Technology; Brno University of Technology; University of Illinois System; University of Illinois Urbana-Champaign
摘要：Self-assembly (SA) plays a pivotal role in nanotechnology, offering cost-effective methods for bottom-up fabrication and providing versatile model systems for investigating fundamental interactions in various bioinspired systems. However, current methods for investigating and quantifying the dynamics of SA systems are limited in their applicability to planar interfaces, particularly in liquid environments. These methods typically rely on analyzing the collective behavior of particle suspension...
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作者：Huang, Yida; Lin, Huimin; Shi, Jiahao; Chen, Yifan; Sang, Qi; Wang, Ruimin; Liu, Wanshan; Wu, Jiao; Li, Yanyan; Xu, Xiaoyu; Ding, Chunmeng; Yang, Shouzhi; Zhang, Juxiang; Jia, Renbing; Fan, Xianqun; Qian, Kun; Zhou, Yixiong
作者单位：Shanghai Jiao Tong University; Shanghai Jiao Tong University; Shanghai Jiao Tong University; Shanghai Jiao Tong University; Shanghai Jiao Tong University
摘要：Ocular adnexal lymphoma (OAL) is the most common orbital malignancy in adults. Advanced tools for precise diagnosis and prognosis of OAL are in demand. Here, the nanoparticle-enhanced laser desorption/ionization mass spectrometry was applied for the construction of OAL-associated serum metabolic patterns (SMPs) from 239 participants (104 OAL and 135 non-OAL). Through machine learning, the diagnostic performance with an area-under-the-curve (AUC) of 0.901 was achieved for OAL based on SMPs. Fur...
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作者：Richardson, Reese A. K.; Hong, Spencer S.; Byrne, Jennifer A.; Stoeger, Thomas; Amaral, Luis A. Nunes
作者单位：Northwestern University; Northwestern University; Northwestern University; University of Sydney; Northwestern University; Northwestern University; Northwestern University; Feinberg School of Medicine; Northwestern University; Northwestern University
摘要：Science is characterized by collaboration and cooperation, but also by uncertainty, competition, and inequality. While there has always been some concern that these pressures may compel some to defect from the scientific research ethos-i.e., fail to make genuine contributions to the production of knowledge or to the training of an expert workforce-the focus has largely been on the actions of lone individuals. Recently, however, reports of coordinated scientific fraud activities have increased....
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作者：Lin, Yiyan; Parajon, Eleana; Yuan, Qinling; Ye, Siyu; Qin, Guanghui; Deng, Yu; Borleis, Jane; Koyfman, Ariel; Iglesias, Pablo A.; Konstantopoulos, Konstantinos; Robinson, Douglas N.; Devreotes, Peter N.
作者单位：Johns Hopkins University; Johns Hopkins University; Johns Hopkins University; Johns Hopkins University; Johns Hopkins University; Microsoft; Johns Hopkins University; Johns Hopkins University; Johns Hopkins University; Johns Hopkins University; Johns Hopkins University; Johns Hopkins University
摘要：Ras has traditionally been regarded as a positive regulator and therapeutic target due to its role in cell proliferation, but recent findings indicate a more nuanced role in cell migration, where suppressed Ras activity can unexpectedly promote migration. To clarify this complexity, we systematically modulate Ras activity using various RasGEF and RasGAP proteins and assess their effects on migration dynamics. Leveraging optogenetics, we assess the immediate, nontranscriptional effects of Ras s...
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作者：Soom, Sarah; Moning, Stefan Urs; Cook, Gregory M.; Lingford, James P.; Kropp, Ashleigh; Tran, Sieu; Grinter, Rhys; Greening, Chris; von Ballmoos, Christoph
作者单位：University of Bern; University of Bern; University of Otago; Queensland University of Technology (QUT); Monash University; Monash University; University of Melbourne; Monash University; Monash University
摘要：All cells require a continuous supply of the universal energy currency, adenosine triphosphate (ATP), to drive countless cellular reactions. The universally conserved F1Fo-ATP synthase regenerates ATP from ADP and Pi by harnessing a transmembrane electrochemical proton gradient (pmf). Bacteria have evolved diverse pmf-forming strategies using light, organic, and inorganic energy sources. Recently, we proposed that many bacteria survive using atmospheric trace gases to produce ATP when limited ...
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作者：Tang, Weiwei; Yang, Taimin; Tu, Qing; Calderon, Hector A.; Hernandez, Francisco C. Robles; O'Reilly, Padraic; Jing, Yun; Park, Sung; Rimer, Jeffrey D.
作者单位：University of Houston System; University of Houston; Tianjin University; Stockholm University; Texas A&M University System; Texas A&M University College Station; United States Department of Energy (DOE); Lawrence Berkeley National Laboratory
摘要：Understanding and exploiting material flexibility through phenomena such as the bending and twisting of molecular crystals has been a subject of increased interest owing to the number of applications that benefit from these properties, such as optoelectronics, mechanophotonics, soft robotics, and smart sensors. Here, we report the growth of spontaneously bent and twisted ammonium urate crystals induced by the keto-enol tautomerism of the urate molecule. The major tautomer is native to biogenic...
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作者：Owens, Gregory L.; Cai, Zhe; Bercovich, Natalia; Todesco, Marco; Lee-Yaw, Julie A.; Rieseberg, Loren H.
作者单位：University of Victoria; University of British Columbia; University of British Columbia; University of British Columbia; University of British Columbia; University of Ottawa
摘要：The cytoplasmic genomes of plants and animals often fail to track species boundaries. However, the mechanisms responsible for such patterns are poorly understood, in part because few studies have linked cytoplasmic variation to phenotypic traits or environmental differences. Here, we use 1,554 previously published and 185 new whole genome sequences representing 14 taxa from the sunflower genus Helianthus, 91 phenotypic traits measured in common gardens, and 39 environmental variables to test f...
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作者：Jankovic, Matija; Kang, Seungmin; Bhattacharya, Sarnab; Kashanchi, Jordon; Wang, Jieting; Deoli, Kanika; Huang, Tianda; Johnson, Alex; Ambani, Karina; Kim, Sangjun; Wang, Pulin; Coyle, Edward; Lu, Nanshu
作者单位：University of Texas System; University of Texas Austin; University of Texas System; University of Texas Austin; University of Texas System; University of Texas Austin; University of Texas System; University of Texas Austin; University of Texas System; University of Texas Austin; University of Texas System; University of Texas Austin
摘要：Assessment of whole-body hydration (WBH) is crucial for health management and disease diagnosis. Traditional methods are invasive or require bulky equipment, making them impractical for mobile, continuous sensing. We present a wearable bioimpedance sensor with strategic electrode placement across the arm for noninvasive, continuous, and mobile WBH monitoring. Although whole-body bioimpedance is a proven method for indicating WBH, the effectiveness of local bioimpedance measurement has been unc...
