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作者：Lai, Youbo; Zhang, Tengteng; Huang, Ling; Klymchenko, Andrey S.; Lin, Weiying
作者单位：Guangxi University; Universites de Strasbourg Etablissements Associes; Universite de Strasbourg; Centre National de la Recherche Scientifique (CNRS)
摘要：Monitoring subcellular organelle dynamics in real time and precisely assessing membrane heterogeneity in living cells are very important for studying fundamental biological mechanisms and gaining a comprehensive understanding of cellular processes. However, there remains a shortage of effective tools for these purposes. Herein, we propose a strategy to develop the exchangeable water- sensing probeAPBD for time- lapse imaging of dynamics in cellular membrane- bound organelle morphology with str...
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作者：Li, Cong; Li, Minglai; Zhang, Lin; Li, Qiang; Zheng, Hua; Feldman, Marcus W.
作者单位：Xi'an Jiaotong University; City University of Hong Kong; City University of Hong Kong; Chinese Academy of Sciences; Research Center for Eco-Environmental Sciences (RCEES), CAS; Stanford University
摘要：Affordable and clean energy, eliminating poverty, and reducing inequality are important goals of the United Nations Sustainable Development Goals (SDGs). This paper examines the role of access to clean cooking fuels in promoting income growth and reducing income inequality. Using data from Chinese households, we show that a 10% increase in the adoption of clean cooking fuels would result in an increase in total annual household income of US$37 billion nationwide. Income growth from access to c...
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作者：Stadnicki, Emily J.; Ludewig, Hannes; Kumar, Ramasamy P.; Wang, Xicong; Qiao, Youwei; Kern, Dorothee; Bradshaw, Niels
作者单位：Brandeis University; University of Massachusetts System; University of Massachusetts Worcester; Scripps Research Institute; Scripps Research Institute; Howard Hughes Medical Institute; Brandeis University; Howard Hughes Medical Institute
摘要：Reversible protein phosphorylation directs essential cellular processes including cell division, cell growth, cell death, inflammation, and differentiation. Because protein phosphorylation drives diverse diseases, kinases and phosphatases have been targets for drug discovery, with some achieving remarkable clinical success. Most protein kinases are activated by phosphorylation of their activation loops, which shifts the conformational equilibrium of the kinase toward the active state. To turn ...
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作者：Kakade, Aishwarya Arun; Gupta, Sachin; Johnson, Andrea; Varghese, Reshmi; Adicherla, Harikrishna; Nagarkar-Jaiswal, Sonal
作者单位：Council of Scientific & Industrial Research (CSIR) - India; CSIR - Centre for Cellular & Molecular Biology (CCMB); Academy of Scientific & Innovative Research (AcSIR)
摘要：To unveil the molecular players that maintain neural stem cell (NSC) homeostasis, we conducted a genetic screen in Drosophila and isolated an uncharacterized gene that we named Inseparable (Insep). Insep is the Drosophila homologue of human IER3IP1, a gene associated with Microcephaly, Epilepsy, and Neonatal Diabetes Syndrome-1 (MEDS-1). We show that Insep loss leads to early larval lethality with small brains and these phenotypes can be rescued by expressing IER3IP1 indicating that their biol...
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作者：Korchak, Emilie J.; Sharafi, Mona; Maisonet, Isabella Jaen; Chaparro, Andres Salazar -; V. Semenova, Irina; Khan, Hamza; O'Neil, Alison L.; Caro, Pilar; Schaaf, Christian P.; Buhrlage, Sara J.; Bezsonova, Irina
作者单位：University of Connecticut; Harvard University; Harvard University Medical Affiliates; Dana-Farber Cancer Institute; Harvard University; Harvard Medical School; Wesleyan University; Wesleyan University; Ruprecht Karls University Heidelberg
摘要：Hao-Fountain syndrome is a rare neurodevelopmental disorder caused by mutations in the deubiquitinating enzyme Ubiquitin-Specific Protease 7 (USP7). Due to the novelty of the disease and its poorly understood molecular mechanisms, treatments for the syndrome are currently lacking. This study examines the effects of 11 patient-derived variants located within the catalytic domain of USP7, focusing on their impact on the enzyme's activity, thermodynamic stability, and substrate recognition. Our f...
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作者：Kuromiya, Kaori; Kobayashi, Yuya; Hirano, Masato; Furuya, Shinichi
作者单位：Sony Corporation
摘要：Creativity in the arts, such as painting and musical performance, hinges on the ability to produce a wide spectrogram of perceptual experiences. In music, it has long been believed that the timbre of tones can be altered by nuanced movements of performers. Previous studies have described relationships between fundamental elements of auditory perceptions (e.g., loudness, tempo) and physical movements (e.g., force, speed), but it remains unknown whether and how delicate features of perceptual ex...
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作者：Moriyama, Miyu; Rodrigues, Gisele; Wang, Jiping; Jayewickreme, Radeesha; Hudak, Andrew; Dong, Huiping; Homer, Robert J.; Ma, Shuangge; Iwasaki, Akiko
作者单位：Yale University; Yale University; Yale University; Yale University; Yale University; Howard Hughes Medical Institute
摘要：Licensed parenteral influenza vaccines induce systemic antibody responses and alleviate disease severity but do not efficiently induce local mucosal immune responses. Here, we describe an intranasal booster strategy with unadjuvanted recombinant hemagglutinin (HA) following initial messenger RNA-lipid nanoparticle (mRNA-LNP) vaccination, Prime and HA. This regimen establishes highly protective HA-specific mucosal immune memory responses in the respiratory tract. Intranasal HA boosters resulted...
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作者：Ren, Jie; Duan, Yaoyuan; Li, Ruoqi; Zhang, Xin; Shi, Yanfei; Zhou, Shiyun; Xie, Kaidong; Wu, Xiaomeng; Irish, Vivian F.; Deng, Xiuxin; Zhang, Fei; Guo, Wenwu
作者单位：Huazhong Agricultural University; Hubei Hongshan Laboratory; Yale University; Yale University
摘要：A common characteristic of thorns, prickles, and spines is a hardened and sharp tip. This feature complicates cultivation and postharvest processing for many crops, yet the molecular mechanisms governing this specific sclerification process remain unclear. By genome editing screening, we identified a MYB family gene named SHORT and SOFT THORN 1 (SST1) that specifically promotes the development of sclerenchyma cells at Citrus thorn tips. CRISPR-Cas9 editing of SST1 results in the formation of s...
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作者：Yang, Ge; Wang, Dong; Liu, Bin
作者单位：University of Minnesota System
摘要：Borna disease virus 1 (BoDV-1), an emerging zoonotic pathogen from the Bornaviridae family, is neurotropic and can infect a variety of mammalian hosts, including humans. Linked to severe encephalitis and high mortality, BoDV-1 currently lacks licensed treatments or vaccines. The BoDV-1 polymerase complex, comprising the large (L) and phosphoprotein (P) subunits, is crucial for viral replication and transcription, making it a promising target for antiviral intervention. Here, we present the cry...
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作者：Chen, Xiangjun; Hwang, Hee Seung; Li, Bihua; Zhao, Yanhua; Ghosh, Koushik; Deng, Lei; Creasey, Elizabeth A.; Ashenberg, Orr; Graham, Daniel B.; Xavier, Ramnik J.
作者单位：Harvard University; Harvard Medical School; Harvard University Medical Affiliates; Massachusetts General Hospital; Harvard University; Massachusetts Institute of Technology (MIT); Broad Institute; Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; Harvard Medical School; Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; Brigham & Women's Hospital; Harvard University; Harvard Medical School
摘要：Nuclear receptors (NRs) are crucial to integrate metabolite sensing and immune responses in the gut. NR- interacting protein 1 (NRIP1) is an important coregulator of various NRs that has been implicated in inflammatory bowel disease risk, but mechanistic details of how NRIP1 controls NR activities mediating immune homeostasis and inflammation remain elusive. We demonstrate that a missense risk tory cytokine production, ultimately leading to exacerbated intestinal inflammation. Mechanistically,...
