Proceedings of the National Academy of Sciences of the United States of America

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• Concerted transport and phosphorylation of diacylglycerol at ER-PM contact sites regulate phospholipid dynamics during stress AI翻译
  作者：Garcia-Hernandez, Selene; Morello-Lopez, Jorge; Haslam, Richard; Amorim-Silva, Vitor; Moya-Cuevas, Jose; Catala, Rafael; Michaelson, Louise; Perez-Sancho, Jessica; Markovic, Vedrana; Salinas, Julio; Napier, Johnathan; Jaillais, Yvon; Ruiz-Lopez, Noemi; Botella, Miguel A.
  作者单位：Consejo Superior de Investigaciones Cientificas (CSIC); Universidad de Malaga; UK Research & Innovation (UKRI); Biotechnology and Biological Sciences Research Council (BBSRC); Rothamsted Research; Consejo Superior de Investigaciones Cientificas (CSIC); CSIC - Centro de Investigaciones Biologicas (CIB); Centre National de la Recherche Scientifique (CNRS); Ecole Normale Superieure de Lyon (ENS de LYON); Universite Claude Bernard Lyon 1; INRAE
  摘要：A universal response of plants to environmental stresses is the activation of plasma membrane (PM) phospholipase C, which hydrolyzes phosphoinositides to produce soluble inositol phosphate and diacylglycerol (DAG). Because of their conical shape, DAG amounts have to be tightly regulated or they can destabilize membranes. We previously showed that upon stress, Synaptotagmin1 (SYT1) transports DAG from the PM to the endoplasmic reticulum (ER) at ER-PM Contact Sites (CS). Here, we addressed the f...
• Projecting neurons from the lateral entorhinal cortex to the basolateral amygdala mediate the encoding of incidental odor-taste associations AI翻译
  作者：Gonzalez-Parra, Jose Antonio; Acciai, Vittoria; Vidal-Palencia, Laura; Canela- Grimau, Marc; Busquets- Garcia, Arnau
  作者单位：Hospital del Mar Research Institute; Hospital del Mar
  摘要：Since our first steps in life, we are forming incidental associations between diverse stimuli across various sensory modalities that influence our future choices and facilitate adaptation to environmental fluctuations. Daily behavior is usually governed by indirect incidental associations among sensory cues that have never been explicitly paired with a reinforcer. This phenomenon, known as higher-order conditioning, can be systematically investigated in laboratory animals through specific beha...
• Single-cell resolution uncovers neighboring cell subtypes that share steroidogenic capacity during fetal testis development AI翻译
  作者：Jiang, Keer; Fu, Zirui; Tsourkas, Philippos; Kothandapani, Anbarasi; Kearse, Tyler; McIlwain, Sean J.; Mayere, Chloe; Nef, Serge; Jorgensen, Joan S.
  作者单位：University of Wisconsin System; University of Wisconsin Madison; University of Wisconsin System; University of Wisconsin Madison; University of Wisconsin System; University of Wisconsin Madison; University of Geneva; University of Geneva
  摘要：Historically, endocrine cells were perceived to coordinate their output in a uniform manner. Recently however, single-cell technologies have uncovered heterogeneity within these populations, indicating that individual cells may operate as independently regulated units. Using high-resolution tools such as single-molecule fluorescent in situ hybridization (sm-FISH) and single-cell RNA sequencing (scRNA-seq), we investigated the contributions of individual and the collective of fetal Leydig cells...
• Detection of the knee point in lithium- ion battery degradation using a state- of- charge- dependent parameter AI翻译
  作者：Kim, Hyunjae; Kim, Inwoo; Kim, Minsoo; An, Seongha; Ahn, Hyo Chul; Park, Dongmin; Lee, Jun Hee; Kang, Chun Yong; Choi, Jang Wook
  作者单位：Seoul National University (SNU); Seoul National University (SNU); Seoul National University (SNU); Hyundai Motors
  摘要：The rapidly expanding lithium- ion battery (LIB) market has heightened the demand for efficient diagnostics for in- use cells and the reliable grading of used cells. Various purpose- built analysis tools and statistical algorithms have been developed, but often rely on redundant instrumentation and computationally intensive procedures. Here, we propose using the variance of the capacity difference between 0.2C and 1C, Var(Delta Q0.2C- 1C(V)), based on the strong correlation between the interfa...
• Evolution of the real area of contact during laboratory earthquakes AI翻译
  作者：Wu, Baoning; Barbot, Sylvain
  作者单位：University of Southern California; University of California System; University of California San Diego; Scripps Institution of Oceanography
  摘要：Empirical slip-rate-and state-dependent friction laws and linear fracture mechanics constitute popular approaches to explaining earthquakes. However, the physics underlying friction laws remain elusive and fracture mechanics does not specify fault strength at the various conditions relevant to crustal faulting. Here, we introduce a physical constitutive framework that augments the traditional approaches by incorporating the real area of contact as the state variable. The physical model explain...
• Establishment of cell size-dependent growth rate via differential scaling of metabolite uptake and release AI翻译
  作者：Meliala, Ian; Chen, Jingyuan; Lyu, Jiahang; Bjorklund, Mikael
  作者单位：Zhejiang University; University of Edinburgh
  摘要：Metabolism fuels cell growth and functions. While it is well established that cellular growth rate scales with cell size, how cells alter their metabolism as they change size remains largely unexplored. Here, we conducted a systematic analysis of cell size-dependent metabolism across the NCI60 cancer cell line panel comprising a diverse range of cell sizes. We demonstrate that cellular metabolism and growth rate display 2/3 allometric scaling due to differential scaling of overall nutrient upt...
• Alternative cGAS signaling promotes herpes simplex encephalitis AI翻译
  作者：Shmuel-Galia, Liraz; Jiang, Zhaozhao; Stine, Laurel; Cahill, Sara; Ng, Sze - Ling; Wilson, Ruth; Kandasamy, Richard Kumaran; Kurt-Jones, Evelyn A.; Ramanjulu, Joshi M.; Bertin, John; Kasparcova, Viera; Pesiridis, G. Scott; Fitzgerald, Katherine A.; Humphries, Fiachra
  作者单位：University of Massachusetts System; University of Massachusetts Worcester; UMass Chan Medical School; GlaxoSmithKline; Glaxosmithkline USA; Norwegian University of Science & Technology (NTNU); Norwegian University of Science & Technology (NTNU); University of Massachusetts System; UMass Chan Medical School; University of Massachusetts Worcester; Sanofi-Aventis; Sanofi USA
  摘要：During infection, foreign DNA is sensed by cyclic GMP-AMP synthase (cGAS) leading to the production of cGAMP, STING-dependent type I interferon and proinflammatory cytokine expression, and autophagy. To prevent a response to self-DNA, cGAS activity is tightly regulated. Dysregulation of cGAS underpins interferonopathies, such as Aicardi-Gouti & egrave;res syndrome, as well as Lupus and neurodegenerative diseases like Parkinson's disease. Thus, cGAS and its product cGAMP are therapeutic targets...
• A combined experimental and computational analysis of mantATP turnover in skinned muscle fibers AI翻译
  作者：Montesel, Mauro; De Napoli, Cosimo; Schmidt, Luisa; Germinario, Elena; Guzman, Ulises H.; V. Olsen, Jesper; Marcucci, Lorenzo; Nogara, Leonardo
  作者单位：University of Padua; Veneto Institute Molecular Medicine; University of Copenhagen; University of Padua
  摘要：Myosin is the primary motor protein in skeletal muscle, responsible for adenosine triphosphate (ATP) hydrolysis that drives muscle contraction. In addition to force production, resting myosin consumes ATP in futile cycles at two rates, the slower one being associated with the Super Relaxed State (SRX), in contrast to the less inhibited Disordered Relaxed State (DRX). The SRX is typically measured using the mantATP chasing technique, where the decay of a fluorescent ATP analogue is fitted using...
• Aerobic nitrogen cycle 100 My before permanent atmospheric oxygenation AI翻译
  作者：Uveges, Benjamin T.; Izon, Gareth; Junium, Christopher K.; Ono, Shuhei; Summons, Roger E.
  作者单位：Massachusetts Institute of Technology (MIT); Cornell University; Syracuse University
  摘要：The Great Oxidation Event (GOE) represents a major shift in Earth's surficial redox balance. Delineating the driver(s) and tempo of the GOE and its impact on microbial evolution and biogeochemistry can be aided by characterizing the cycling of redox-sensitive elements such as nitrogen. While previous studies have shown that the transition to a broadly aerobic marine nitrogen cycle occurred in step with the final phase of the GOE similar to 2.33 billion years ago (Ga), an evolving understanding...
• Modes of action of a small molecule antiviral compound targeting yellow fever virus NS4B protein AI翻译
  作者：Wang, Fuxuan; Gao, Zhao; Chen, Bo; Jiang, Zhengyuan; Renner, David M.; Li, Jiaqi; Tolufashe, Gideon; Du, Yanming; Guo, Ju-Tao; Chang, Jinhong
  摘要：from the invagination of endoplasmic reticulum membranes, designated as replication organelles (ROs). Nonstructural protein 4B (NS4B) of flaviviruses play essential roles in the biogenesis of ROs and evasion of innate immune responses. We report herein that only rapidly inhibits YFV RNA synthesis, but also induces the activation of cytoplasmic double- stranded RNA (dsRNA)- sensing pathways to accelerate the apoptosis of infected cells. Genetic analyses revealed that all the three cytoplasmic d...