Proceedings of the National Academy of Sciences of the United States of America

最新文章

• Measurement of adhesion and traction of cells at high yield reveals an energetic ratchet operating during nephron condensation AI翻译
  作者：Liu, Jiageng; Prahl, Louis S.; Huang, Aria Zheyuan; Hughes, Alex J.
  作者单位：University of Pennsylvania; University of Pennsylvania; University of Pennsylvania; University of Pennsylvania; University of Pennsylvania; University of Pennsylvania; University of Pennsylvania; University of Pennsylvania
  摘要：Developmental biology- inspired strategies for tissue- building have extraordinary promise for regenerative medicine, spurring interest in the relationship between cell biophysical properties and morphological transitions. However, mapping gene or protein expression data to cell biophysical properties to physical morphogenesis remains challenging with current techniques. Here, we present multiplexed adhesion and traction of cells at high yield (MATCHY). MATCHY advances the multiplexing and thr...
• The telencephalon is a neuronal substrate for systemic inflammatory responses in teleosts via polyamine metabolism AI翻译
  作者：Mani, Amir; Haddad, Farah; Barreda, Daniel R.; Salinas, Irene
  作者单位：University of New Mexico; University of Alberta; University of Alberta
  摘要：Systemic inflammation elicits sickness behaviors and fever by engaging a complex neuronal circuitry that begins in the preoptic area of the hypothalamus. Ectotherms such as teleost fish display sickness behaviors in response to infection or inflammation, seeking warmer temperatures to enhance survival via behavioral fever responses. To date, the hypothalamus is the only brain region implicated in sickness behaviors and behavioral fever in teleosts. Yet, the complexity of neurobehavioral manife...
• Neural network architecture of a mammalian brain AI翻译
  作者：Swanson, Larry W.; Hahn, Joel D.; Sporns, Olaf
  作者单位：University of Southern California; Indiana University System; Indiana University Bloomington; Indiana University System; Indiana University Bloomington
  摘要：Connectomics research is making rapid advances, although models revealing general lished connection reports indicates that the adult rat brain interregional connectome has about 76,940 of a possible 623,310 axonal connections between its 790 gray matter regions mapped in a reference atlas, equating to a network density of 12.3%. We examined the sexually dimorphic network using multiresolution consensus clustering that generated a nested hierarchy of interconnected modules/subsystems with three...
• Single- cell analysis identifies distinct macrophage phenotypes associated with prodisease and proresolving functions in the endometriotic niche AI翻译
  作者：Henlon, Yasmin; Panir, Kavita; Mcintyre, Iona; Hogg, Chloe; Dhami, Priya; Cuff, Antonia O.; Senior, Anna; Adwani, Niky Moolchandani -; Courtois, Elise T.; Horne, Andrew W.; Rosser, Matthew; Ott, Sascha; Greaves, Erin
  作者单位：University of Warwick; University of Warwick; University of Edinburgh; Jackson Laboratory
  摘要：Endometriosis negatively impacts the health- related quality of life of 190 million women worldwide. Novel advances in nonhormonal treatments for this debilitating condition are desperately needed. Macrophages play a vital role in the pathophysiology of endometriosis and represent a promising therapeutic target. In the current study, we revealed the full transcriptomic complexity of endometriosis- associated macrophage subpopulations using single- cell analyses in a preclinical mouse model of ...
• IRoning out mosquitoes' attraction to mugginess AI翻译
  作者：Montell, Craig
  作者单位：University of California System; University of California Santa Barbara; University of California System; University of California Santa Barbara
• Multifunctional biomolecular corona- inspired nanoremediation of antibiotic residues AI翻译
  作者：Hou, Jie; Lu, Yuqi; Chen, Qiqi; Liao, Xinyi; Wu, Xinyue; Sang, Kaijian; White, Jason C.; Gardea-Torresdey, Jorge L.; Xu, Jiang; Zhang, Jianying; Yang, Kun; Zhu, Lizhong; Lin, Daohui
  作者单位：Zhejiang University; Zhejiang University; Connecticut Agricultural Experiment Station; University of Texas System; University of Texas El Paso; Zhejiang University
  摘要：Facing complex and variable emerging antibiotic pollutants, the traditional development of functional materials is a trial- and- error process based on physicochemical principles, where laborious steps and long timescales make it difficult to accelerate technical breakthroughs. Notably, natural biomolecular coronas derived from highly tolerant organisms under significant contamination scenarios can be used in conjunction with nanotechnology to tackling emerging contaminants of concern. Here, s...
• Conserved 5-methyluridine tRNA modification modulates ribosome translocation AI翻译
  作者：Jones, Joshua D.; Franco, Monika K.; Giles, Rachel N.; Eyler, Daniel E.; Tardu, Mehmet; Smith, Tyler J.; Snyder, Laura R.; Polikanov, Yury S.; Kennedy, Robert T.; Niederer, Rachel O.; Koutmou, Kristin S.
  作者单位：University of Michigan System; University of Michigan; University of Michigan System; University of Michigan; University of Illinois System; University of Illinois Chicago; University of Illinois Chicago Hospital; University of Michigan System; University of Michigan
  摘要：While the centrality of posttranscriptional modifications to RNA biology has long been acknowledged, the function of the vast majority of modified sites remains to be discovered. Illustrative of this, there is not yet a discrete biological role assigned for one of the most highly conserved modifications, 5- methyluridine at position 54 in tRNAs (m5U54). Here, we uncover contributions of m5U54 to both tRNA maturation and protein synthesis. Our mass spectrometry analyses demonstrate that cells l...
• An artificially evolved gene for herbicide- resistant rice breeding AI翻译
  作者：Dong, Jin; Yu, Xin-He; Dong, Jiangqing; Wang, Gao-Hua; Wang, Xin-Long; Wang, Da-Wei; Yan, Yao-Chao; Xiao, Han; Ye, Bao-Qin; Lin, Hong-Yan; Yang, Guang-Fu
  作者单位：Central China Normal University; Central China Normal University; Hubei University of Chinese Medicine
  摘要：Discovering and engineering herbicide- resistant genes is a crucial challenge in crop breeding. This study focuses on the 4- hydroxyphenylpyruvate dioxygenase Inhibitor Sensitive 1- Like (HSL) protein, prevalent in higher plants and exhibiting weak catalytic activity against many beta- triketone herbicides (beta- THs). The crystal structures of maize HSL1A complexed with beta- THs were elucidated, identifying four essential herbicide- binding residues and explaining the weak activity of HSL1A ...
• Extended time, elevated expectations: The unappreciated downsides of pausing the tenure clock AI翻译
  作者：Holland, Maria A.; Maisel, Katharina; Ibberson, Carolyn B.; Wiley, Laura K.; Burnett, David C.; Mace, Emily M.; Elting, Mary Williard
  作者单位：University of Notre Dame; University System of Maryland; University of Maryland College Park; University of Tennessee System; University of Tennessee Knoxville; University of Colorado System; University of Colorado Anschutz Medical Campus; Portland State University; Columbia University; NewYork-Presbyterian Hospital; North Carolina State University
• An AAGAB-to-CCDC32 handover mechanism controls the assembly of the AP2 adaptor complex AI翻译
  作者：Wan, Chun; Puscher, Harrison; Ouyang, Yan; Wu, Jingyi; Tian, Yuan; Li, Suzhao; Yin, Qian; Shen, Jingshi
  作者单位：University of Colorado System; University of Colorado Boulder; State University System of Florida; Florida State University; State University System of Florida; Florida State University; University of Colorado System; University of Colorado Anschutz Medical Campus
  摘要：Vesicular transport relies on multimeric trafficking complexes to capture cargo and drive vesicle budding and fusion. Faithful assembly of the trafficking complexes is essential to their functions but remains largely unexplored. Assembly of AP2 adaptor, a heterotetrameric protein complex regulating clathrin- mediated endocytosis, is assisted by the chaperone AAGAB. Here, we found that AAGAB initiates AP2 assembly by stabilizing its alpha and sigma 2 subunits, but the AAGAB:alpha:sigma 2 comple...