An artificially evolved gene for herbicide- resistant rice breeding

• 成果类型: Article
• 署名作者: Dong, Jin; Yu, Xin-He; Dong, Jiangqing; Wang, Gao-Hua; Wang, Xin-Long; Wang, Da-Wei; Yan, Yao-Chao; Xiao, Han; Ye, Bao-Qin; Lin, Hong-Yan; Yang, Guang-Fu
• 署名单位: Central China Normal University; Central China Normal University; Hubei University of Chinese Medicine
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-12323
• DOI: 10.1073/pnas.2407285121
• 发表日期: 2024-08-20
• 关键词: 4-hydroxyphenylpyruvate dioxygenase; broad-spectrum; agrobacterium; TRANSFORMATION; DISCOVERY; synthase

摘要

Discovering and engineering herbicide- resistant genes is a crucial challenge in crop breeding. This study focuses on the 4- hydroxyphenylpyruvate dioxygenase Inhibitor Sensitive 1- Like (HSL) protein, prevalent in higher plants and exhibiting weak catalytic activity against many beta- triketone herbicides (beta- THs). The crystal structures of maize HSL1A complexed with beta- THs were elucidated, identifying four essential herbicide- binding residues and explaining the weak activity of HSL1A against the herbicides. Utilizing an artificial evolution approach, we developed a series of rice HSL1 mutants targeting the four residues. Then, these mutants were systematically evaluated, identifying the M10 variant as the most effective in modifying beta- THs. The initial active conformation of substrate binding in HSL1 was also revealed from these mutants. Furthermore, overexpression of M10 in rice significantly enhanced resistance to beta- THs, resulting in a notable 32- fold increase in resistance to methyl- benquitrione. In conclusion, the artificially evolved M10 gene shows great potential for the development of herbicide- resistant crops.