Proceedings of the National Academy of Sciences of the United States of America

最新文章

• Mechanistic insights into the small-molecule inhibition of influenza A virus entry AI翻译
  作者：Xu, Yan; Anirudhan, Varada; Gaisina, Irina N.; Du, Haijuan; Alqarni, Saad; Moore, Terry W.; Caffrey, Michael; Manicassamy, Balaji; Zhou, Tongqing; Rong, Lijun; Xu, Kai
  作者单位：University System of Ohio; Ohio State University; University of Illinois System; University of Illinois Chicago; University of Illinois Chicago Hospital; University of Illinois System; University of Illinois Chicago; University of Illinois Chicago Hospital; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID); University Ha'il; University of Illinois System; University of Illinois Chicago; University of Illinois Chicago Hospital; University of Illinois System; University of Illinois Chicago; University of Illinois Chicago Hospital; University of Iowa; University System of Ohio; Ohio State University
  摘要：Influenza A virus (IAV) is a zoonotic pathogen responsible for seasonal and pandemic flu. The extensive genetic and antigenic diversity within and between IAV phylogenetic groups presents major challenges for developing universal vaccines and broad-spectrum antiviral therapies. Current interventions provide limited protection due to the virus's high mutation rate and capacity for immune evasion. Recent advancements in viral hemagglutinin (HA)-targeting small-molecule entry inhibitors offer a p...
• Solution spillover: Pairing issues with ideological solutions fuels polarization AI翻译
  作者：Kafka, Adrienne M.; Campbell, Troy H.; Kay, Aaron C.
  作者单位：Duke University
  摘要：Recent research has focused on understanding ideological and affective political polarization in the United States; however, the polarization of issue salience-defined as personal beliefs about how important or concerning an issue is, and how much attention or priority it deserves-has garnered less attention, especially regarding its underlying causes. We propose that issue salience polarization, particularly that of ideologically neutral issues, is driven in part by two psychological processe...
• Endocannabinoids inhibit contextual fear memory generalization via hippocampal GABAergic synaptic transmission AI翻译
  作者：Ge, Qian; Zhang, Jinming; Huo, Qing; Yu, Jing; Zheng, Qiaohua; Qian, Zhaoqiang; Wei, Chunling; Jiang, Tongtong; Liu, Yihui; Liu, Zhiqiang; Ren, Wei; Sun, Zongpeng; Jia, Zhengping; Han, Jing
  作者单位：Shaanxi Normal University; Air Force Medical University; Shaanxi Normal University; University of Toronto; Hospital for Sick Children (SickKids)
  摘要：Memory generalization allows an organism to adapt to new conditions, but overgeneralization of fear or traumatic experiences can be detrimental to survival and contributes to the development of various mental disorders. However, the cellular and molecular mechanisms underlying fear memory generalization, especially in the hippocampus, remain largely unknown. In this study, utilizing a well-established mouse model of fear memory generalization, we investigated the role of endocannabinoids (eCBs...
• Tree rings reveal persistent Western Apache (Ndee) fire stewardship and niche construction in the American Southwest AI翻译
  作者：Roos, Christopher I.; Kaib, J. Mark; Laluk, Nicholas C.; Adams, Melinda M.; Guiterman, Christopher H.; Baisan, Christopher H.; Morino, Kiyomi; Swetnam, Thomas W.
  作者单位：Southern Methodist University; United States Department of the Interior; US Fish & Wildlife Service; University of Arizona; University of California System; University of California Berkeley; University of Kansas; University of Kansas; University of Colorado System; University of Colorado Boulder; National Oceanic Atmospheric Admin (NOAA) - USA
  摘要：Identifying the influence of low-density Indigenous populations in paleofire records has been methodologically challenging. In the Southwest United States, well-replicated fire histories suggest that abundant lightning and suitable climate conditions drove frequent low-severity wildfires in dry pine forests independent of human activities even as ethnography provided hints that highly mobile indigenous populations used fire in myriad land use contexts. Here, we leverage published and unpublish...
• Cryosectioning-enhanced super-resolution microscopy for single-protein imaging across cells and tissues AI翻译
  作者：Stein, Johannes; Ericsson, Maria; Nofal, Michel; Magni, Lorenzo; Aufmkolk, Sarah; Mcmillan, Ryan B.; Breimann, Laura; Herlihy, Conor P.; Lee, S. Dean; Willemin, Andrea; Wohlmann, Jens; Arguedas-Jimenez, Laura; Yin, Peng; Pombo, Ana; Church, George M.; Wu, Chao-ting
  作者单位：Harvard University; Harvard Medical School; Harvard University; Harvard Medical School; Helmholtz Association; Max Delbruck Center for Molecular Medicine; Humboldt University of Berlin; University of Oslo
  摘要：DNA-points accumulation for imaging in nanoscale topography (DNA-PAINT) enables nanoscale imaging with virtually unlimited multiplexing and molecular counting. Here, we address challenges, such as variable imaging performance and target accessibility, that can limit its broader applicability. Specifically, we enhance its capacity for robust single-protein imaging and molecular counting by optimizing the integration of total internal reflection fluorescence microscopy with physical sectioning, ...
• Actions of the antiseizure drug carbamazepine in the thalamic reticular nucleus: Potential mechanism of aggravating absence seizures AI翻译
  作者：Jang, Sung-Soo; Agranonik, Nicole; Huguenard, John R.
  作者单位：Stanford University
  摘要：Carbamazepine (CBZ) is a widely used antiepileptic drug effective in managing partial and generalized tonic-clonic seizures. Despite its established therapeutic efficacy, CBZ has been reported to worsen seizures in another form of epilepsy, generalized absence seizures, in both clinical and experimental settings. In this study, we focused on thalamic reticular (RT) neurons, which regulate thalamocortical network activity in absence seizures, to investigate whether CBZ alters their excitability...
• Ciprofloxacin- driven purifying selection on viral genomes accelerates soil N2O production AI翻译
  作者：Fang, Linfa; Lakshmanan, Prakash; Zhang, Hailin; Deng, Yue; Xiao, Ran; Wen, Teng; Ma, Bin; Ge, Tida; Mueller, Christoph; Zhang, Jinbo; Chen, Xinping; Zhu, Yongguan; Su, Xiaoxuan
  作者单位：Southwest University - China; Southwest University - China; Guangxi Academy of Agricultural Sciences; University of Queensland; Nanjing Normal University; Zhejiang University; Ningbo University; Justus Liebig University Giessen; Justus Liebig University Giessen; University College Dublin; University College Dublin; Chinese Academy of Sciences
  摘要：Viruses are ubiquitous regulators of microbial dynamics and may thus greatly influence global microbial- driven greenhouse gas emissions. Anthropogenic stressors, such as chemical contamination, are likely to amplify these viral contributions; however, their global significance and underlying mechanisms remain elusive. Utilizing 15N tracing, metagenomics, and laboratory assays, we explore soil viral communities and their evolutionary potential under the stress from antibiotic ciprofloxacin (CI...
• Synergy between membrane topography and domains to control signaling protein localization in mast cells facilitates their activation AI翻译
  作者：Ghosh, Shirsendu; Wiesner, Alice Wagenknecht -; Desai, Shriya; Vyphuis, Jada; Ramos, Mariena Silvestry; Grazul, John L.; Baird, Barbara A.
  作者单位：Cornell University; Cornell University
  摘要：Similar to T cells and B cells, mast cell surfaces are dominated by microvilli, and like these other immune cells we showed with microvillar cartography (MVC) that key signaling proteins for RBL mast cells localize to these topographical features. Although stabilization of ordered lipid nanodomains around antigen-crosslinked IgE-Fc epsilon RI is known to facilitate necessary coupling with Lyn tyrosine kinase to initiate transmembrane signaling in these mast cells, the relationship of ordered-l...
• QnAs with Hans F. Coetzee AI翻译
  作者：Hardcastle, Matthew
• A versatile immunomodulated nanoCRISPR converter augments the susceptibility and visibility of tumors to the immune system AI翻译
  作者：Li, Yingjie; Zhou, Shiyao; Fu, Wangxian; Li, Xinchao; Chen, Tao; Le, Hao; Xu, Yangsong; Tang, Yuting; Mi, Peng; Gao, Huile; Wu, Qinjie; Gong, Changyang
  作者单位：Sichuan University; Sichuan University; Sichuan University; Sichuan University
  摘要：Immune checkpoint blockade (ICB) has potential in alleviating cytotoxic T lymphocyte (CTL) exhaustion. However, resistance that impaired major histocompatibility complex class I (MHC-I) expression on tumors can be developed in many patients after ICB treatment, resulting in insufficient antigen presentation to CTLs. Herein, we rationally design a versatile and powerful immunomodulated hierarchical nanoCRISPR converter (SWITCH) targeting PD-L1 and PCSK9 loci to convert the immune-resistance sta...