Proceedings of the National Academy of Sciences of the United States of America

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• Cryo- EM structure of cyanopodophage A4 reveals a pentameric pre- ejectosome in the double- stabilized capsid AI翻译
  作者：Hou, Pu; Zhou, Rui-Qian; Jiang, Yong-Liang; Yu, Rong-Cheng; Du, Kang; Gan, Nanqin; Ke, Fei; Zhang, Qi-Ya; Li, Qiong; Zhou, Cong-Zhao
  作者单位：Chinese Academy of Sciences; University of Science & Technology of China, CAS; Chinese Academy of Sciences; University of Science & Technology of China, CAS; Chinese Academy of Sciences; University of Science & Technology of China, CAS; Chinese Academy of Sciences; Institute of Hydrobiology, CAS
  摘要：Upon infection, the podophages usually eject a couple of proteins from the capsid to form a transmembrane ejectosome on the host cell membrane that facilitates the ejection of viral genome. However, it remains unclear how these proteins of pre-ejectosome are finely assembled at the center of highly packaged genome. Here, we report the intact structure of Anabaena cyanopodophage A4, which consists of a capsid stabilized by two types of cement proteins and a short tail attached with six tail fib...
• AcrIE7 inhibits the CRISPR- Cas system by directly binding to the R- loop single- stranded DNA AI翻译
  作者：Kim, Do Yeon; Lee, So Yeon; Ha, Hyun Ji; Park, Hyun Ho
  作者单位：Chung Ang University
  摘要：The CRISPR-Cas system is a well-known adaptive immune system in bacteria, and a prominent mechanism for evading this immunity involves anti-CRISPR (Acr) proteins, which employ various methods to neutralize the CRISPR-Cas system. In this study, using structural and biochemical analyses, we revealed that AcrIE7 binds to the single-stranded DNA in the R-loop formed when Cascade encounters the target DNA, thereby preventing Cas3 from cleaving the DNA. This represents a different inhibition strateg...
• Explaining human motor coordination via the synergy expansion hypothesis AI翻译
  作者：Tessari, Federico; West Jr, A. Michael; Hogan, Neville
  作者单位：Massachusetts Institute of Technology (MIT); Johns Hopkins University; Massachusetts Institute of Technology (MIT)
  摘要：The search for an answer to Bernstein's degrees of freedom problem has propelled a large portion of research studies in human motor control over the past six decades. Different theories have been developed to explain how humans might use their incredibly complex neuro-musculo-skeletal system with astonishing ease. Among these theories, motor synergies appeared as one possible explanation. In this work, the authors investigate the nature and role of synergies and propose a theoretical framework...
• Cholesterol- dependent enzyme activity of human TSPO1 AI翻译
  作者：Qiu, Weihua; Trinh, Thi Kim Hoang; Catalano, Claudio; Mehta, Akul; Desai, Umesh R.; Kellogg, Glen E.; Hendrickson, Wayne A.; Guo, Youzhong
  作者单位：Virginia Commonwealth University; Virginia Commonwealth University; Columbia University; Columbia University
  摘要：The amino acid sequence of the tryptophan- rich sensory proteins (TSPO) is substantially conserved throughout all kingdoms of life. Human mitochondrial TSPO1 (HsTSPO1) binds to porphyrins and steroids, although its interactions with these molecules remains unknown. HsTSPO1 is associated with numerous physiological and pathological disorders, but the underlying molecular mechanisms are unknown. Here, we disclose the finding of human mitochondrial TSPO as a cholesterol- dependent protoporphyrin ...
• Prediction of phase-separation propensities of disordered proteins from sequence AI翻译
  作者：von Buelow, Soeren; Tesei, Giulio; Kamalzaidi, Fatima; Mittag, Tanja; Lindorff-Larsen, Kresten
  作者单位：University of Copenhagen; St Jude Children's Research Hospital
  摘要：Phase separation is one possible mechanism governing the selective cellular enrichment of biomolecular constituents for processes such as transcriptional activation, mRNA regulation, and immune signaling. Phase separation is mediated by multivalent interactions of macromolecules including intrinsically disordered proteins and regions (IDRs). Despite considerable advances in experiments, theory, and simulations, the prediction of the thermodynamics of IDR phase behavior remains challenging. We ...
• Supporting Information for Walters et al., Identification of FSH- regulated and estrous stage-specific transcriptional networks in mouse ovaries (vol 112, e2411977122,2025) AI翻译
  作者：Walters, Kathryn; Baldwin, Amber; Liu, Zhenghui; Larsen, Mark; Mukherjee, Neelanjan; Kumar, T. Rajendra
• High- density CRISPRi screens reveal diverse routes to improved acclimation in cyanobacteria AI翻译
  作者：Hren, Andrew; Lollini, Nicole; Carper, Dana L.; Abraham, Paul E.; Cameron, Jeffrey C.; Fox, Jerome M.; Eckert, Carrie A.
  作者单位：University of Colorado System; University of Colorado Boulder; United States Department of Energy (DOE); Oak Ridge National Laboratory; University of Colorado System; University of Colorado Boulder; University of Colorado System; University of Colorado Boulder; United States Department of Energy (DOE); National Renewable Energy Laboratory - USA
  摘要：Cyanobacteria are the oldest form of photosynthetic life on Earth and contribute to primary production in nearly every habitat, from permafrost to hot springs. Despite longstanding interest in the acclimation of these microbes, it remains poorly understood and challenging to rewire. This study uses a high- density, genome- wide CRISPR interference screen to examine the influence of gene- specific transcriptional variation on the growth of Synechococcus sp. PCC 7002 under environmental extremes...
• p53 activates circASCC3 to repress R- loops and enhance resistance to chemotherapy AI翻译
  作者：Cao, Mingming; Gan, Yu; Huang, Yingdan; Tong, Jing; Xiong, Chen; Chen, Yajie; Chen, Bing; Huang, Ruixuan; Xie, Bangxiang; Deng, Jun; Huang, Shenglin; He, Xianghuo; Hao, Qian; Zhou, Xiang
  作者单位：Fudan University; Fudan University; Fudan University; Fudan University; Nanchang University; Capital Medical University; Fudan University; Fudan University
  摘要：The tumor suppressor p53 can trigger tumor resistance to chemotherapy by facilitating DNA damage repair and maintaining genomic integrity. Here, we report that a p53- induced circular RNA circASCC3 promotes chemotherapeutic resistance by resolving R- loops. Our results reveal that p53 directly activates the transcription of is identified to inhibit the formation of circASCC3 by associating with its flanking regions. Importantly, p53 facilitates the formation of circASCC3 by repressing the expr...
• Astrocytes in the mouse brain respond bilaterally to unilateral retinal neurodegeneration AI翻译
  作者：Cooper, Melissa L.; Gildea, Holly K.; Selles, Maria Clara; Katafygiotou, Eleni; Liddelow, Shane A.; Chao, Moses, V
  作者单位：New York University; New York University; NYU Langone Medical Center; NYU Langone Medical Center
  摘要：Glaucomatous optic neuropathy, or glaucoma, is the world's primary cause of irreversible blindness. Glaucoma is comorbid with other neurodegenerative diseases, but how it might impact the environment of the full central nervous system to increase neurodegenerative vulnerability is unknown. Two neurodegenerative events occur early in the optic nerve, the structural link between the retina and brain: loss of anterograde transport in retinal ganglion cell (RGC) axons and early alterations in astr...
• Positive effects of species mixing on biodiversity of understory plant communities and soil health in forest plantations AI翻译
  作者：Guo, Jiahuan; Kneeshaw, Daniel; Peng, Changhui; Wu, Yaoxing; Feng, Lei; Qu, Xinjing; Wang, Weifeng; Pan, Chang; Feng, Huili
  作者单位：Hainan University; University of Quebec; University of Quebec Montreal; Hunan Normal University; International Centre for Bamboo & Rattan; Nanjing University; Nanjing Forestry University; Anqing Normal University
  摘要：Mixed- species plantations are increasingly recognized for their potential to maintain forest biodiversity and soil health; however, a comprehensive assessment of their global effectiveness is lacking. To fill this knowledge gap, we conducted a meta- analysis of 7,045 paired observations between mixed- species and monoculture plantations, derived from 311 studies across diverse forest ecosystems worldwide. Our results show that mixed- species plantations significantly increased understory plan...