Proceedings of the National Academy of Sciences of the United States of America

最新文章

• miR-155 impairs ICOSL and MHC- I expression in DLBCL lymphomas AI翻译
  作者：Tili, Esmerina; Commisso, Teresa L.; Balatti, Veronica; Michaille, Jean - Jacques; Nuovo, Gerard J.; Croce, Carlo M.
  作者单位：University System of Ohio; Ohio State University; University System of Ohio; Ohio State University; James Cancer Hospital & Solove Research Institute
  摘要：Elevated miR-155 levels in B cell malignancies, such as CLL and DLBCL, correlate with increased aggressiveness of the disease. We recently reported that, in two different mouse models of miR-155-driven B cell malignancy, miR-155 targets and down-regulates transcripts encoding ICOSL, the ligand for the Inducible T cell costimulator (ICOS), thereby impairing the capacity of T lymphocytes to recognize and eliminate malignant cells. In report, we extend our previous findings to Human by showing th...
• Structural basis of ZP2-targeted female nonhormonal contraception AI翻译
  作者：Dioguardi, Elisa; Stsiapanava, Alena; Fahrenkamp, Eileen; Han, Ling; de Sanctis, Daniele; Inzunza, Jose; Jovine, Luca
  作者单位：Karolinska Institutet; Karolinska Institutet; Chiesi Pharmaceuticals Inc
  摘要：Monoclonal antibody IE-3 prevents mouse fertilization by binding ZP2, a major component of the oocyte-specific zona pellucida (ZP). We show that an IE-3-derived single-chain variable fragment (scFV) is sufficient for blocking fertilization in vitro and determine the structural basis of IE-3/ZP2 recognition. The high affinity of this interaction depends on induced fit of the epitope, offering insights for nonhormonal contraceptive design without off-target effects.
• Indigenous Knowledge as a sole data source in habitat selection functions AI翻译
  作者：Gryba, Rowenna; Von Duyke, Andrew; Huntington, Henry P.; Adams, Billy; Frantz, Brower; Gatten, Justin; Harcharek, Qaiyaan; Sarren, Robert; Henry, Greg; Auger-Methe, Marie
  作者单位：University of British Columbia; University of British Columbia; University of British Columbia
  摘要：While Indigenous Knowledge (IK) contains a wealth of information on the behavior and habitat use of species, it is rarely included in the species-habitat models frequently used by Western species management authorities. As decisions from these authorities can limit access to species that are important culturally and for subsistence, exclusion of IK in conservation and management frameworks can negatively impact both species and Indigenous communities. In partnership with I & ntilde;upiat hunte...
• Richardson's law and the origins of alcohol research AI翻译
  作者：Mukerjee, Snigdha; Siciliano, Cody A.
  作者单位：Vanderbilt University
  摘要：Interest in the biological actions of alcohols, ethanol in particular, dates back to the earliest historical texts. Alcohol research is now a highly active field with roots in physiology, pharmacology, toxicology, and neuroscience. But at what point did interest and speculation evolve into bona fide science? Here, we set out to identify the earliest systematic empirical investigations into the biological actions of alcohols and unearthed a surprisingly rigorous literature which included a fund...
• Design of a halogen bond catalyzed DNA endonuclease AI翻译
  作者：Walker, Margaret G.; Mendez, Cesar Gustavo; Ho, Alexander N.; Czarny, Ryan S.; Rappe, Anthony K.; Ho, Pui Shing
  作者单位：Colorado State University System; Colorado State University Fort Collins; Colorado State University System; Colorado State University Fort Collins
  摘要：In this study, we expand the repertoire of biological catalysts by showing that a halogen bond (X-bond) can functionally replace the magnesium (Mg2+) cofactor in mouse endonuclease G (mEndoG). We mutated the metal coordinating glutamate E136 in mEndoG to a meta-halotyrosine (mXY, X = chlorine or iodine) to form a mXY-mEndoG construct that is both acid and base catalyzed. Under basic conditions, the enzyme is inactivated by the metal chelator ethylene diamine tetraacetic acid (EDTA), indicating...
• An atlas of protein phosphorylation dynamics during interferon signaling AI翻译
  作者：Busnadiego, Idoia; Lork, Marie; Fernbach, Sonja; Schiefer, Samira; Tsolakos, Nikos; Hale, Benjamin G.
  作者单位：University of Zurich; University of Copenhagen
  摘要：Interferons (IFNs, types I-III) have pleiotropic functions in promoting antiviral and antitumor responses, as well as in modulating inflammation. Dissecting the signaling mechanisms elicited by different IFNs is therefore critical to understand their phenotypes. Here, we use mass spectrometry to investigate the early temporal dynamics of cellular protein phosphorylation in a human lung epithelial cell-line as it responds to stimulation with IFN alpha 2, IFN beta, IFN omega, IFN gamma, or IFN l...
• Correction for Ganguli et al., A culture- free biphasic approach for sensitive and rapid detection of pathogens in dried whole- blood matrix AI翻译
  作者：Ganguli, Anurup; Lim, Jongwon; Mostafa, Ariana; Saavedra, Carlos; Rayabharam, Archith; Aluru, Narayana R.; Wester, Matthew; White, Karen C.; Kumar, James; McGuffin, Reubin; Frederick, Ann; Valera, Enrique; Bashir, Rashid
• The FBXW7-KMT 2 axis in cancer- associated fibroblasts controls tumor growth via an epigenetic- paracrine mechanism AI翻译
  作者：Yin, Lu; Zhang, Jiagui; Zhu, Zhipeng; Peng, Xiaojuan; Lan, Huiyin; Ayoub, Alex; Tan, Mingjia; Zhou, Bo; He, Yaohui; Wang, Siyuan; Lu, Yan; Liu, Wen; Xiong, Xiufang; Huang, Jing; Dou, Yali; Mao, Fengbiao; Sun, Yi
  作者单位：Zhejiang University; Zhejiang University; Shanghai Jiao Tong University; University of Michigan System; University of Michigan; University of Michigan System; University of Michigan; University of Michigan System; University of Michigan; Xiamen University; Zhejiang University; Zhejiang University; Zhejiang University; University of Southern California; University of Southern California; Zhejiang University; Zhejiang University
  摘要：F-box and WD repeat domain-containing 7 (FBXW7) is a tumor suppressor that targets various oncoproteins for degradation, but its role in modulating cancer-associated fibroblasts (CAFs) in the tumor microenvironment remains elusive. Here, we report that FBXW7 expression is gradually downregulated in CAFs during the progression of human pancreatic and lung cancers. Mechanically, FBXW7 inhibits histone lysine methyltransferase 2 (KMT2) methyltransferase activity via retinoblastoma binding protein...
• Painting rich six- dimensional pictures using polarized fluorescence microscopy AI翻译
  作者：Lew, Matthew D.
  作者单位：Washington University (WUSTL)
• Dopaminergic neurons in the paraventricular hypothalamus extend the food consumption phase AI翻译
  作者：Ariyani, Winda; Yoshikawa, Chiharu; Tsuneoka, Haruka; Amano, Izuki; Imayoshi, Itaru; Ichinose, Hiroshi; Sumi-Ichinose, Chiho; Koibuchi, Noriyuki; Kitamura, Tadahiro; Kohno, Daisuke
  作者单位：Gunma University; Gunma University; Kyoto University; Kyoto University; Kyoto University; Fujita Health University
  摘要：Feeding behavior is controlled by various neural networks in the brain that are involved in different feeding phases: Food procurement, consumption, and termination. However, the specific neural circuits controlling the food consumption phase remain poorly understood. Here, we investigated the roles of dopaminergic neurons in the paraventricular nucleus of the hypothalamus (PVH) in the feeding behavior in mice. Our results indicated that the PVH dopaminergic neurons were critical for extending...