21期

最新文章

• Dichotomous transactivation domains contribute to growth inhibitory and promotion functions of TAp73 AI翻译
  作者：Li, Dan; Kok, Catherine Yen Li; Wang, Chao; Ray, Debleena; Osterburg, Susanne; Doetsch, Volker; Ghosh, Sujoy; Sabapathy, Kanaga
  作者单位：National Cancer Centre Singapore (NCCS); National University of Singapore; Goethe University Frankfurt; Goethe University Frankfurt; Goethe University Frankfurt; National University of Singapore; National University of Singapore; Nanyang Technological University
  摘要：The transcription factor p73, a member of the p53 tumor - suppressor family, regulates cell death and also supports tumorigenesis, although the mechanistic basis for the dichotomous functions is poorly understood. We report here the identification of an alternate transactivation domain (TAD) located at the extreme carboxyl (C) terminus of TAp73 (3 , a commonly expressed p73 isoform. Mutational disruption of this TAD significantly reduced TAp73 (3 's transactivation activity, to a level observe...
• Synergistic induction of blood-brain barrier properties AI翻译
  作者：Porkolab, Gergo; Meszaros, Maria; Szecsko, Aniko; Vigh, Judit P.; Walter, Fruzsina R.; Figueiredo, Ricardo; Kalomista, Ildiko; Hoyk, Zsofia; Vizsnyiczai, Gaszton; Grof, Ilona; Jan, Jeng-Shiung; Gosselet, Fabien; Pirity, Melinda K.; Vastag, Monika; Hudson, Natalie; Campbell, Matthew; Veszelka, Szilvia; Deli, Maria A.
  作者单位：HUN-REN; HUN-REN Biological Research Center; Institute of Biophysics - HAS; Szeged University; Gedeon Richter Chemistry Works; National Cheng Kung University; Universite d'Artois; HUN-REN; HUN-REN Biological Research Center; Institute of Genetics - HAS; Trinity College Dublin
  摘要：Blood-brain barrier (BBB) models derived from human stem cells are powerful tools to improve our understanding of cerebrovascular diseases and to facilitate drug development for the human brain. Yet providing stem cell-derived endothelial cells with the right signaling cues to acquire BBB characteristics while also retaining their vascular identity remains challenging. Here, we show that the simultaneous activation of cyclic AMP and Wnt/beta-catenin signaling and inhibition of the TGF-beta pat...
• Essential role of N- terminal SAM regions in STIM1 multimerization and function AI翻译
  作者：Sallinger, Matthias; Humer, Christina; Ong, Hwei Ling; Narayanasamy, Sasirekha; Lin, Qi Tong; Fahrner, Marc; Grabmayr, Herwig; Berlansky, Sascha; Choi, Sean; Schmidt, Tony; Maltan, Lena; Atzgerstorfer, Lara; Niederwieser, Martin; Frischauf, Irene; Romanin, Christoph; Stathopulos, Peter B.; Ambudkar, Indu; Leitner, Romana; Borinenry, Daniel; Schind, Rainer
  作者单位：Johannes Kepler University Linz; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR); Western University (University of Western Ontario); Medical University of Graz; University of Luxembourg
  摘要：The single - pass transmembrane protein Stromal Interaction Molecule 1 (STIM1), located in the endoplasmic reticulum (ER) membrane, possesses two main functions: It senses the ER - Ca 2+ concentration and directly binds to the store - operated Ca 2+ channel Orai1 for its activation when Ca 2+ recedes. At high resting ER - Ca 2+ concentration, the ER - luminal STIM1 domain is kept monomeric but undergoes di/multimerization once stores are depleted. Luminal STIM1 multimerization is essential to ...
• Lipid- derived electrophiles inhibit the function of membrane channels during ferroptosis AI翻译
  作者：Van Kessel, Antonius T. M.; Cosa, Gonzalo
  作者单位：McGill University; McGill University
  摘要：The therapeutic targeting of ferroptosis requires full understanding of the molecular mechanism of this regulated cell death pathway. While lipid - derived electrophiles (LDEs), including 4 - hydroxy - 2 - nonenal (4 - HNE), are important biomarkers of ferroptosis, a functional role for these highly reactive species in ferroptotic cell death execution has not been established. Here, through mechanistic characterization of LDE - detoxification impairment, we demonstrate that LDEs mediate altere...
• Real- time emulation of future global warming reveals realistic impacts on the phenological response and quality deterioration in rice AI翻译
  作者：Itoh, Hironori; Yamashita, Hiroto; Wada, Kaede C.; Yonemaru, Jun- ichi
  作者单位：National Agriculture & Food Research Organization - Japan; National Agriculture & Food Research Organization - Japan; Shizuoka University
  摘要：Decreased production of crops due to climate change has been predicted scientifically. While climate - resilient crops are necessary to ensure food security and support sustainable agriculture, predicting crop growth under future global warming is challenging. Therefore, we aimed to assess the impact of realistic global warming conditions on rice cultivation. We developed a crop evaluation platform, the agro - environment (AE) emulator, which generates diverse environments by implementing the ...
• Targeting the Plasmodium falciparum UCHL3 ubiquitin hydrolase using chemically constrained peptides AI翻译
  作者：King, Harry R.; Bycroft, Mark; Nguyen, Thanh-Binh; Kelly, Geoff; Vinogradov, Alexander A.; Rowling, Pamela J. E.; Stott, Katherine; Ascher, David B.; Suga, Hiroaki; Itzhaki, Laura S.; Artavanis -Tsakonas, Katerina
  作者单位：University of Cambridge; University of Cambridge; University of Queensland; Francis Crick Institute; University of Tokyo; University of Cambridge
  摘要：The ubiquitin-proteasome system is essential to all eukaryotes and has been shown to be critical to parasite survival as well, including Plasmodium falciparum, the causative agent of the deadliest form of malarial disease. Despite the central role of the ubiquitin-proteasome pathway to parasite viability across its entire life- cycle, specific inhibitors targeting the individual enzymes mediating ubiquitin attachment and removal do not currently exist. The ability to disrupt P. falciparum grow...
• Macrophage transplantation rescues RNASET2-deficient leukodystrophy by replacing deficient microglia in a zebrafish model AI翻译
  作者：Rutherford, Holly A.; Candeias, Diogo; Duncan, Christopher J. A.; Renshaw, Stephen A.; Hamilton, Noemie
  作者单位：University of Sheffield; University of Sheffield; University of York - UK; University of York - UK; Newcastle University - UK; Newcastle University - UK; Newcastle Upon Tyne Hospitals NHS Foundation Trust
  摘要：RNASET2 - deficient leukodystrophy is a rare infantile white matter disorder mimicking a viral infection and resulting in severe psychomotor impairments. Despite its severity, there is little understanding of cellular mechanisms of pathogenesis and no treatments. Recent research using the rnaset2 mutant zebrafish model has suggested that microglia may be the drivers of the neuropathology, due to their failure to digest apoptotic debris during neurodevelopment. Therefore, we developed a strateg...
• Coordinating plasmid partition with bacterial chromosome segregation AI翻译
  作者：Fommell, Barbara E.
  作者单位：University of Toronto
• MACSPI enables tissue- selective proteomic and interactomic analyses in multicellular organisms AI翻译
  作者：Huang, Siyue; Ran, Qiao; Li, Xiao-Meng; Bao, Xiucong; Zheng, Chaogu; Li, Xiang David
  作者单位：University of Hong Kong; University of Hong Kong; University of Hong Kong
  摘要：Multicellular organisms are composed of many tissue types that have distinct morphologies and functions, which are largely driven by specialized proteomes and interactomes. To define the proteome and interactome of a specific type of tissue in an intact animal, we developed a localized proteomics approach called Methionine Analog - based Cell - Specific Proteomics and Interactomics (MACSPI). This method uses the tissue - specific expression of an engineered methionyl - tRNA synthetase to label...