21期

最新文章

• Making evidence go further: Advancing synergy between agent- based modeling and randomized control trials AI翻译
  作者：Hammond, Ross A.; Barkin, Shari
  作者单位：Washington University (WUSTL); Brookings Institution; The Santa Fe Institute; Virginia Commonwealth University; Virginia Commonwealth University
• iATPSnFR2: A high-dynamic-range fluorescent sensor for monitoring intracellular ATP AI翻译
  作者：Marvin, Jonathan S.; Kokotos, Alexandros C.; Kumar, Mukesh; Pulido, Camila; Tkachuk, Ariana N.; Yao, Jocelyn Shuxin; Brown, Timothy A.; Ryan, Timothy A.
  作者单位：Howard Hughes Medical Institute; Cornell University; Weill Cornell Medicine; Johns Hopkins University
  摘要：We developed a significantly improved genetically encoded quantitative adenosine triphosphate (ATP) sensor to provide real - time dynamics of ATP levels in subcellular compartments. iATPSnFR2 is a variant of iATPSnFR1, a previously developed sensor that has circularly permuted superfolder green fluorescent protein (GFP) inserted between the ATP - binding helices of the epsilon - subunit of a bacterial F-0 - F-1 ATPase. Optimizing the linkers joining the two domains resulted in a similar to fiv...
• Negotiating discord in sustainability transformations AI翻译
  作者：Pattersona, James J.; Feolaa, Giuseppe; Kima, Rakhyun E.
  作者单位：Utrecht University
  摘要：Policy action for sustainability transformation faces inherent and ever-present sources of conflict, pushback, and resistance (i.e., discord). However, conceptual frameworks and policy prescriptions for sustainability transformations often reflect an undue image of accord. This involves simplified assumptions about consensus, steering, friction, discreteness, and additiveness of policy action, conferring an unrealistic view of the potential to deliberately realize transformation. Instead, nego...
• Premeiotic 24-nt phasiRNAs are present in the Zea genus and unique in biogenesis mechanism and molecular function AI翻译
  作者：Zhan, Junpeng; Belanger, Sebastien; Lewis, Scott; Teng, Chong; McGregor, Madison; Beric, Aleksandra; Schon, Michael A.; Nodine, Michael D.; Meyers, Blake C.
  作者单位：Huazhong Agricultural University; Hubei Hongshan Laboratory; Donald Danforth Plant Science Center; James Hutton Institute; Washington University (WUSTL); University of California System; University of California Davis; University of California System; University of California Davis; University of Missouri System; University of Missouri Columbia; Wageningen University & Research
  摘要：Reproductive phasiRNAs (phased, small interfering RNAs) are broadly present in angiosperms and play crucial roles in sustaining male fertility. While the premeiotic 21-nt (nucleotides) phasiRNAs and meiotic 24-nt phasiRNA pathways have been extensively studied in maize (Zea mays) and rice (Oryza sativa), a third putative category of reproductive phasiRNAs-named premeiotic 24-nt phasiRNAs-have recently been reported in barley (Hordeum vulgare) and wheat (Triticum aestivum). To determine whether...
• YdbH and YnbE form an intermembrane bridge to maintain lipid homeostasis in the outer membrane of Escherichia coli AI翻译
  作者：Kumar, Sujeet; Davis, Rebecca M.; Ruiz, Natividad
  作者单位：University System of Ohio; Ohio State University
  摘要：The outer membrane (OM) of didermic gram - negative bacteria is essential for growth, maintenance of cellular integrity, and innate resistance to many antimicrobials. Its asymmetric lipid distribution, with phospholipids in the inner leaflet and lipopolysaccharides (LPS) in the outer leaflet, is required for these functions. Lpt proteins form a transenvelope bridge that transports newly synthesized LPS from the inner membrane (IM) to OM, but how the bulk of phospholipids are transported betwee...
• Interpersonal heart rate synchrony predicts effective information processing in a naturalistic group decision-making task AI翻译
  作者：Sharika, K. M.; Thaikkandi, Swarag; Nivedita; Platt, Michael L.
  作者单位：Indian Institute of Technology System (IIT System); Indian Institute of Technology (IIT) - Kanpur; Indian Institute of Technology System (IIT System); Indian Institute of Technology (IIT) - Kanpur; University of Oxford; University of Pennsylvania; University of Pennsylvania; University of Pennsylvania; University of Pennsylvania
  摘要：Groups often outperform individuals in problem -solving. Nevertheless, failure to critically evaluate ideas risks suboptimal outcomes through so-called groupthink. Prior studies have shown that people who hold shared goals, perspectives, or understanding of the environment show similar patterns of brain activity, which itself can be enhanced by consensus -building discussions. Whether shared arousal alone can predict collective decision -making outcomes, however, remains unknown. To address th...
• Clocking out and letting go to unleash green biotech applications in a photosynthetic host AI翻译
  作者：Xu, Yao; Jabbur, Maria Luisa; Mori, Tetsuya; Young, Jamey D.; Johnson, Carl Hirschie
  作者单位：Vanderbilt University; Vanderbilt University
  摘要：Cyanobacteria are photosynthetic bacteria whose gene expression patterns are globally regulated by their circadian (daily) clocks. Due to their ability to use sunlight as their energy source, they are also attractive hosts for green production of pharmaceuticals, renewable fuels, and chemicals. However, despite the application of traditional genetic tools such as the identification of strong promoters to enhance the expression of heterologous genes, cyanobacteria have lagged behind other micro...
• Microbes vary strategically in their metalation of mononuclear enzymes AI翻译
  作者：Rohaun, Sanjay Kumar; Sethu, Ramakrishnan; Imlay, James A.
  作者单位：University of Illinois System; University of Illinois Urbana-Champaign
  摘要：Studies have determined that nonredox enzymes that are cofactored with Fe(II) are the most oxidant-sensitive targets inside Escherichia coli. These enzymes use Fe(II) cofactors to bind and activate substrates. Because of their solvent exposure, the metal can be accessed and oxidized by reactive oxygen species, thereby inactivating the enzyme. Because these enzymes participate in key physiological processes, the consequences of stress can be severe. Accordingly, when E. coli senses elevated lev...
• PM 2.5 exposure contributes to anxiety and depression- like behaviors via phenyl- containing compounds interfering with dopamine receptor AI翻译
  作者：Ji, Shaoyang; Guo, Yuqiong; Yan, Wei; Ding, Jinjian; Hong, Wenjun; Wu, Xiaoyun; Ku, Tingting; Yue, Huifeng; Sang, Nan; Murphy, Catherine
  作者单位：Shanxi University; Xuzhou Medical University; China Jiliang University; China Jiliang University
  摘要：As a global problem, fine particulate matter (PM 2.5 ) really needs local fixes. Considering the increasing epidemiological relevance to anxiety and depression but inconsistent toxicological results, the most important question is to clarify whether and how PM 2.5 causally contributes to these mental disorders and which components are the most dangerous for crucial mitigation in a particular place. In the present study, we chronically subjected male mice to a real - world PM 2.5 exposure syste...
• Neuronal PAS domain 1 identifies a major subpopulation of wakefulness- promoting GABAergic neurons in the basal forebrain AI翻译
  作者：Troppoli, Timothy A.; Yang, Chun; Katsuki, Fumi; Uygun, David S.; Lin, Ilyan; Aguilar, David D.; Spratt, Tristan; Basheer, Radhika; Mcnally, James M.; Chan, C. Savio; Mckenna, James T.; Brown, Ritchie E.
  作者单位：Harvard University; Harvard University Medical Affiliates; US Department of Veterans Affairs; Veterans Health Administration (VHA); VA Boston Healthcare System; Harvard University; Harvard Medical School; Northwestern University; Feinberg School of Medicine
  摘要：Here, we describe a group of basal forebrain (BF) neurons expressing neuronal Per-Arnt-Sim (PAS) domain 1 (Npas1), a developmental transcription factor linked to neuropsychiatric disorders. Immunohistochemical staining in Npas1- cre-2A- TdTomato mice revealed BF Npas1+ neurons are distinct from well- studied parvalbumin or cholinergic neurons. Npas1 staining in GAD67-GFP knock - in mice confirmed that the vast majority of Npas1+ neurons are GABAergic, with minimal colocalization with glutamate...