-
作者：Shi, Lei; Derouiche, Abderahmane; Pandit, Santosh; Alazmi, Meshari; Ventroux, Magali; Kohler, Julie Bonne; Noirot-Gros, Marie-Francoise; Gao, Xin; Mijakovic, Ivan
作者单位：Chalmers University of Technology; King Abdullah University of Science & Technology; University Ha'il; King Abdullah University of Science & Technology; Universite Paris Saclay; INRAE; AgroParisTech; Technical University of Denmark
摘要：In bacteria, attenuation of protein - tyrosine phosphorylation occurs during oxidative stress. The main described mechanism behind this effect is the H 2 O 2 - triggered conversion of bacterial phosphotyrosines to protein - bound 3,4 - dihydroxyphenylalanine. This disrupts the bacterial tyrosine phosphorylation - based signaling network, which alters the bacterial polysaccharide biosynthesis. Herein, we report an alternative mechanism, in which oxidative stress leads to a direct inhibition of ...
-
作者：Vanderplas, Susan; Carriquiry, Alicia; Hofmann, Heike
作者单位：University of Nebraska System; University of Nebraska Lincoln; Iowa State University
摘要：When wires are cut, the tool produces striations on the cut surface; as in other forms of forensic analysis, these striation marks are used to connect the evidence to the source that created them. Here, we argue that the practice of comparing two wire cut surfaces introduces complexities not present in better-investigated forensic examination of toolmarks such as those observed on bullets, as wire comparisons inherently require multiple distinct comparisons, increasing the expected false disco...
-
作者：Kochanowsky, Joshua A.; Mira, Portia M.; Elikaee, Samira; Muratore, Katherine; Rai, Anand Kumar; Riestra, Angelica M.; Johnson, Patricia J.
作者单位：University of California System; University of California Los Angeles; California State University System; San Diego State University
摘要：Trichomonas vaginalis , a common sexually transmitted parasite that colonizes the human urogenital tract, secretes extracellular vesicles (TvEVs) that are taken up by human cells and are speculated to be taken up by parasites as well. While the crosstalk between TvEVs and human cells has led to insight into host:parasite interactions, roles for TvEVs in infection have largely been one- sided, with little known about the effect of TvEV uptake by T. vaginalis . Approximately 11% of infections ar...
-
作者：Ceolini, Enea; Ridderinkhof, K. Richard; Ghosh, Arko; Carpenter, Stephen
作者单位：Leiden University; Leiden University - Excl LUMC; University of Amsterdam
摘要：We experience a life that is full of ups and downs. The ability to bounce back after adverse life events such as the loss of a loved one or serious illness declines with age, and such isolated events can even trigger accelerated aging. How humans respond to common day-to-day perturbations is less clear. Here, we infer the aging status from smartphone behavior by using a decision tree regression model trained to accurately estimate the chronological age based on the dynamics of touchscreen inte...
-
作者：Herrera, Alfa; Packer, Megan M.; Lemus, Monica Rosas-; Minasov, George; Chen, Jiexi; Brumell, John H.; Satchell, Karla J. F.
作者单位：Northwestern University; Feinberg School of Medicine; Northwestern University; Feinberg School of Medicine; University of Toronto; Hospital for Sick Children (SickKids); University of Toronto; University of Toronto; University of Toronto; Hospital for Sick Children (SickKids); University of New Mexico
摘要：Vibrio vulnificus causes life- threatening wound and gastrointestinal infections, mediated primarily by the production of a Multifunctional- Autoprocessing Repeats - In - Toxin (MARTX) toxin. The most commonly present MARTX effector domain, the Makes Caterpillars Floppy - like (MCF) toxin, is a cysteine protease stimulated by host adenosine diphosphate (ADP) ribosylation factors (ARFs) to autoprocess. Here, we show processed MCF then binds and cleaves host Ras- related proteins in brain (Rab) ...
-
作者：Rhana, Paula; Matsumoto, Collin; Fong, Zhihui; Costa, Alexandre D.; Del Villar, Silvia G.; Dixon, Rose E.; Santana, L. Fernando
作者单位：University of California System; University of California Davis
摘要：The heart beats approximately 100,000 times per day in humans, imposing substantial energetic demands on cardiac muscle. Adenosine triphosphate (ATP) is an essential energy source for normal function of cardiac muscle during each beat, as it powers ion transport, intracellular Ca2+ handling, and actin-myosin cross-bridge cycling. Despite this, the impact of excitation-contraction coupling on the intracellular ATP concentration ([ATP](i)) in myocytes is poorly understood. Here, we conducted rea...
-
作者：Guillen, Jaresley V.; Glaunsinger, Britt A.
作者单位：University of California System; University of California Berkeley; University of California System; University of California Berkeley; Howard Hughes Medical Institute
-
作者：Smolen, Gromoslaw A.; Sordella, Raffaella; Muir, Beth; Mohapatra, Gayatry; Barmettler, Anne; Archibald, Heidi; Kim, Woo J.; Okimoto, Ross A.; Bell, Daphne W.; Sgroi, Dennis C.; Christensen, James G.; Settleman, Jeffrey; Haber, Daniel A.
-
作者：Windisch, Roland; Soliman, Sarah; Hoffmann, Adrian; Chen-Wichmann, Linping; Danese, Anna; Vosberg, Sebastian; Bravo, Jimena; Lutz, Sebastian; Kellner, Christian; Fischer, Alexander; Gebhard, Claudia; Monte, Enric Redondo; Hartmann, Luise; Schneider, Stephanie; Beier, Fabian; Strobl, Carolin Dorothea; Weigert, Oliver; Peipp, Matthias; Schuendeln, Michael; Stricker, Stefan H.; Rehli, Michael; Bernhagen, Juergen; Humpe, Andreas; Klump, Hannes; Brendel, Christian; Krause, Daniela S.; Greif, Philipp A.; Wichmann, Christian
作者单位：University of Munich; University of Munich; University of Munich; University of Munich; University of Munich; Helmholtz Association; German Cancer Research Center (DKFZ); Helmholtz Association; German Cancer Research Center (DKFZ); Medical University of Graz; Octapharma; University of Regensburg; University of Munich; University of Munich; RWTH Aachen University; RWTH Aachen University Hospital; University of Kiel; University of Duisburg Essen; University of Duisburg Essen; University of Munich; University of Duisburg Essen; RWTH Aachen University; RWTH Aachen University Hospital; Harvard University; Harvard Medical School; Harvard University Medical Affiliates; Dana-Farber Cancer Institute; Boston Children's Hospital; Goethe University Frankfurt; Goethe University Frankfurt Hospital
摘要：Ex vivo expansion of human CD34+ hematopoietic stem and progenitor cells remains a challenge due to rapid differentiation after detachment from the bone marrow niche. In this study, we assessed the capacity of an inducible fusion protein to enable sustained ex vivo proliferation of hematopoietic precursors and their capacity to differentiate into functional phagocytes. We fused the coding sequences of an FK506- Binding Protein 12 (FKBP12)- derived destabilization domain (DD) to the myeloid/lym...
-
作者：Adachi, Urara; Koita, Rina; Seto, Akira; Maeno, Akiteru; Ishizu, Atsuki; Oikawa, Sae; Tani, Taisei; Ishizaka, Mizuki; Yamada, Kazuya; Satoh, Koumi; Nakazawa, Hidemichi; Furudate, Hiroyuki; Kawakami, Koichi; Iwanami, Norimasa; Matsuda, Masaru; Kawamura, Akinori
作者单位：Saitama University; Utsunomiya University; Research Organization of Information & Systems (ROIS); National Institute of Genetics (NIG) - Japan; Research Organization of Information & Systems (ROIS); National Institute of Genetics (NIG) - Japan; Graduate University for Advanced Studies - Japan; Graduate University for Advanced Studies - Japan
摘要：The dorsal and anal fins can vary widely in position and length along the anterior- posterior axis in teleost fishes. However, the molecular mechanisms underlying the diversification of these fins remain unknown. Here, we used genetic approaches in zebrafish and medaka, in which the relative positions of the dorsal and anal fins are opposite, to demonstrate the crucial role of hox genes in the patterning of the teleost posterior body, including the dorsal and anal fins. By the CRISPR - Cas9 - ...
