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作者：Dai, Hengyi; Wu, Binbin; Zhuang, Yajuan; Ren, Hao; Chen, Yanbo; Zhang, Fangzhou; Chu, Chiheng; Lv, Xiaofei; Xu, Jianming; Ma, Bin
作者单位：Zhejiang University; Zhejiang University; Zhejiang University; Zhejiang University; China Jiliang University
摘要：Effective colonization by microbe in the rhizosphere is critical for establishing a beneficial symbiotic relationship with the host plant. Bacillus subtilis, a soil- dwelling bacterium that is commonly found in association with plants and their rhizosphere, has garnered interest for its potential to enhance plant growth, suppress pathogens, and contribute to sustainable agricultural practices. However, research on the dynamic distribution of B. subtilis within the rhizosphere and its interacti...
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作者：Dostalkova, Alzbeta; Krizova, Ivana; Junkova, Petra; Rackova, Jana; Kapisheva, Marina; Novotny, Radim; Danda, Matej; Zvonarova, Karolina; Sinkovec, Larisa; Vecerkova, Katerina; Bednarova, Lucie; Ruml, Tomas; Rumlova, Michaela
作者单位：University of Chemistry & Technology, Prague; Czech Academy of Sciences; Czech Academy of Sciences; Institute of Molecular Genetics of the Czech Academy of Sciences
摘要：We explored how a simple retrovirus, Mason- Pfizer monkey virus (M-PMV) to facilitate its replication process, utilizes DHX15, a cellular RNA helicase, typically engaged in RNA processing. Through advanced genetic engineering techniques, we showed that M-PMV recruits DHX15 by mimicking cellular mechanisms, relocating it from the nucleus to the cytoplasm to aid in viral assembly. This interaction is essential for the correct packaging of the viral genome and critical for its infectivity. Our fi...
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作者：Zhang, Mark G.; Seyedolmohadesin, Maedeh; Mercado, Soraya Hawk; Tauffenberger, Arnaud; Park, Heenam; Finnen, Nerissa; Schroeder, Frank C.; Venkatachalam, Vivek; Sternberg, Paul W.
作者单位：California Institute of Technology; Northeastern University; Cornell University; Boyce Thompson Institute for Plant Research; Cornell University
摘要：Decisions made over long time scales, such as life cycle decisions, require coordinated interplay between sensory perception and sustained gene expression. The Caenorhabditis elegans dauer (or diapause) exit developmental decision requires sensory integration of population density and food availability to induce an all- or- nothing organismal- wide response, but the mechanism by which this occurs remains unknown. Here, we to be critical for dauer exit, perform sensory integration at both the l...
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作者：Asemota, Sarah; Effah, Wendy; Holt, Jeremiah; Johnson, Daniel; Cripe, Linnea; Ponnusamy, Suriyan; Thiyagarajan, Thirumagal; Khosrosereshki, Yekta; Hwang, Dong-Jin; He, Yali; Grimes, Brandy; Fleming, Martin D.; Pritchard, Frances E.; Hendrix, Ashley; Fan, Meiyun; Jain, Abhinav; Choi, Hyo Young; Makowski, Liza; Hayes, D. Neil; Miller, Duane D.; Pfeffer, Lawrence M.; Santhanam, Balaji; Narayanan, Ramesh
作者单位：University of Tennessee System; University of Tennessee Health Science Center; University of Tennessee System; University of Tennessee Health Science Center; University of Tennessee System; University of Tennessee Health Science Center; University of Tennessee System; University of Tennessee Health Science Center; University of Tennessee System; University of Tennessee Health Science Center; University of Texas System; UTMD Anderson Cancer Center; University of Tennessee System; University of Tennessee Health Science Center; University of Tennessee System; University of Tennessee Health Science Center; St Jude Children's Research Hospital; St Jude Children's Research Hospital
摘要：Cancers develop resistance to inhibitors of oncogenes mainly due to target- centric mechanisms such as mutations and splicing. While inhibitors or antagonists force targets to unnatural conformation contributing to protein instability and resistance, activating tumor suppressors may maintain the protein in an agonistic conformation to elicit sustainable growth inhibition. Due to the lack of tumor suppressor agonists, this hypothesis and the mechanisms underlying resistance are not understood. ...
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作者：Liu, Yukun; Koo, Kunmo; Mao, Zugang; Fu, Xianbiao; Hu, Xiaobing; Dravid, Vinayak P.
作者单位：Northwestern University; Northwestern University; Northwestern University; Technical University of Denmark
摘要：Palladium (Pd) catalysts have been extensively studied for the direct synthesis of H2O through the hydrogen oxidation reaction at ambient conditions. This heterogeneous catalytic reaction not only holds considerable practical significance but also serves as a classical model for investigating fundamental mechanisms, including adsorption and reactions between adsorbates. Nonetheless, the governing mechanisms and kinetics of its intermediate reaction stages under varying gas conditions remain el...
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作者：Pandey, Saurabh; Han, Wenyan; Li, Jun; Shepard, Ryan; Wu, Kunwei; Castellano, David; Tian, Qingjun; Dong, Lijin; Li, Yan; Lu, Wei
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作者：Xu, Houlin; Nguyen, Anh Tay; Bazant, Zdenek P.
作者单位：Northwestern University; Northwestern University; Northwestern University
摘要：The 2023 smooth Lagrangian Crack-Band Model (slCBM), inspired by the 2020 invention of the gap test, prevented spurious damage localization during fracture growth by introducing the second gradient of the displacement field vector, named the sprain, as the localization limiter. The key idea was that, in the finite element implementation, the displacement vector and its gradient should be treated as independent fields with the lowest (C0) continuity, constrained by a second-order Lagrange multi...
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作者：Zhang, Huimin; Lu, Haiquan; Xiang, Lisha; Bullen, John W.; Zhang, Chuanzhao; Samanta, Debangshu; Gilkes, Daniele M.; He, Jianjun; Semenza, Gregg L.
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作者：Asor, Roi; Olerinyova, Anna; Burnap, Sean A.; Kushwah, Manish S.; Soltermann, Fabian; Rudden, Lucas S. P.; Hensen, Mario; Vasiljevic, Snezana; Brun, Juliane; Hill, Michelle; Chang, Liu; Dejnirattisai, Wanwisa; Supasa, Piyada; Mongkolsapaya, Juthathip; Zhou, Daming; Stuart, David I.; Screaton, Gavin R.; Degiacomi, Matteo T.; Zitzmann, Nicole; Benesch, Justin L. P.; Struwe, Weston B.; Kukura, Philipp
作者单位：University of Oxford; University of Oxford; University of Oxford; Durham University; University of Oxford; Wellcome Centre for Human Genetics; Chinese Academy of Medical Sciences - Peking Union Medical College; University of Oxford; Mahidol University; University of Oxford; Wellcome Centre for Human Genetics; Oxford University Hospitals NHS Foundation Trust
摘要：Cellular processes are controlled by the thermodynamics of the underlying biomolecular interactions. Frequently, structural investigations use one monomeric binding partner, while ensemble measurements of binding affinities generally yield one affinity representative of a 1:1 interaction, despite the majority of the proteome consisting of oligomeric proteins. For example, viral entry and inhibition in SARS- CoV- 2 involve cell- surface receptor and dimeric antibodies. Here, we reveal that coop...
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作者：Ma, Yayao; Park, Jongchan; Huang, Luzhe; Sen, Chandani; Burri, Samuel; Bruschini, Claudio; Yang, Xilin; Cui, Qi; Cameron, Robert B.; Fishbein, Gregory A.; Gomperts, Brigitte N.; Ozcan, Aydogan; Charbon, Edoardo; Gao, Liang
作者单位：University of California System; University of California Los Angeles; University of California System; University of California Los Angeles; University of California System; University of California Los Angeles; University of California System; University of California Los Angeles; University of California Los Angeles Medical Center; David Geffen School of Medicine at UCLA; Swiss Federal Institutes of Technology Domain; Ecole Polytechnique Federale de Lausanne; University of California System; University of California Los Angeles; University of California Los Angeles Medical Center; David Geffen School of Medicine at UCLA; University of California System; University of California Los Angeles; University of California Los Angeles Medical Center; David Geffen School of Medicine at UCLA; University of California System; University of California Los Angeles; University of California Los Angeles Medical Center; David Geffen School of Medicine at UCLA
摘要：Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging technique that enables the visualization of biological samples at the molecular level by measuring the fluorescence decay rate of fluorescent probes. This provides critical information about molecular interactions, environmental changes, and localization within biological systems. However, creating high- resolution lifetime maps using conventional FLIM systems can be challenging, as it often requires extensive scanning that ...
