Unveiling the DHX15-G-patch interplay in retroviral RNA packaging

• 成果类型: Article
• 署名作者: Dostalkova, Alzbeta; Krizova, Ivana; Junkova, Petra; Rackova, Jana; Kapisheva, Marina; Novotny, Radim; Danda, Matej; Zvonarova, Karolina; Sinkovec, Larisa; Vecerkova, Katerina; Bednarova, Lucie; Ruml, Tomas; Rumlova, Michaela
• 署名单位: University of Chemistry & Technology, Prague; Czech Academy of Sciences; Czech Academy of Sciences; Institute of Molecular Genetics of the Czech Academy of Sciences
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-9732
• DOI: 10.1073/pnas.2407990121
• 发表日期: 2024-10-01
• 关键词: pfizer monkey virus; constitutive transport element; g-patch domain; lariat-intron; helicase; protein; hiv-1; infectivity; activation; binding

摘要

We explored how a simple retrovirus, Mason- Pfizer monkey virus (M-PMV) to facilitate its replication process, utilizes DHX15, a cellular RNA helicase, typically engaged in RNA processing. Through advanced genetic engineering techniques, we showed that M-PMV recruits DHX15 by mimicking cellular mechanisms, relocating it from the nucleus to the cytoplasm to aid in viral assembly. This interaction is essential for the correct packaging of the viral genome and critical for its infectivity. Our findings offer unique insights into the mechanisms of viral manipulation of host cellular processes, highlighting a sophisticated strategy that viruses employ to leverage cellular machinery for their replication. This study adds valuable knowledge to the understanding of viral-host interactions but also suggests a common evolutionary history between cellular processes and viral mechanisms. This finding opens a unique perspective on the export mechanism of intron- retaining mRNAs in the packaging of viral genetic information and potentially develop ways to stop it.