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作者：Gonzalez, Jacqueline E.; Naik, Harnish Mukesh; Oates, Eleanor H.; Dhara, Venkata Gayatri; Mcconnell, Brian O.; Kumar, Swetha; Betenbaugh, Michael J.; Antoniewicz, Maciek R.
作者单位：University of Delaware; Johns Hopkins University; University of Michigan System; University of Michigan
摘要：Mammalian cell culture processes are widely utilized for biotherapeutics production, disease diagnostics, and biosensors, and hence, should be optimized to support robust cell growth and viability. However, toxic by- products accumulate in cultures due to inefficiencies in metabolic activities and nutrient utilization. In this study, we applied comprehensive 13 C stable- isotope tracing of amino acids and glucose to two Immunoglobulin G (IgG) producing Chinese Hamster Ovary (CHO) cell lines to...
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作者：Dragotakes, Quigly; Johnson, Patrick W.; Buras, Matthew R.; Carter, Rickey E.; Joyner, Michael J.; Bloch, Evan M.; Gebo, Kelly A.; Hanley, Daniel F.; Henderson, Jeffrey P.; Pirofski, Liise-Anne; Shoham, Shmuel; Senefeld, Jonathon W.; Tobian, Aaron A. R.; Wiggins, Chad C.; Wright, R. Scott; Paneth, Nigel S.; Sullivan, David J.; Casadevall, Arturo
作者单位：Johns Hopkins University; Johns Hopkins Bloomberg School of Public Health; Mayo Clinic; Mayo Clinic; Mayo Clinic; Johns Hopkins University; Johns Hopkins Medicine; Johns Hopkins University; Johns Hopkins Medicine; Johns Hopkins University; Johns Hopkins Medicine; Washington University (WUSTL); Yeshiva University; University of Illinois System; University of Illinois Urbana-Champaign; Michigan State University; Mayo Clinic; Mayo Clinic; Michigan State University; Michigan State University
摘要：In the Spring of 2020, the United States of America (USA) deployed COVID-19 convalescent plasma (CCP) to treat hospitalized patients. Over 500,000 patients were treated with CCP during the first year of the pandemic. In this study, we estimated the number of actual inpatient lives saved by CCP treatment in the United States of America based on CCP weekly use, weekly national mortality data, and CCP mortality reduction data from meta-analyses of randomized controlled trials and real-world data....
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作者：Jung, Won; Chen, Tai-Yen; Santiago, Ace George; Chen, Peng
作者单位：Cornell University; Harvard University; University of Houston System; University of Houston
摘要：Memory effect refers to the phenomenon where past events influence a system's current and future states or behaviors. In biology, memory effects often arise from intra- or intermolecular interactions, leading to temporally correlated behaviors. Single-molecule studies have shown that enzymes and DNA-binding proteins can exhibit time-correlated behaviors of their activity. While memory effects are well documented and studied in vitro, no such examples exist in cells to our knowledge. Combining ...
