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作者：Kusuma, Subhan Hadi; Kakizuka, Taishi; Hattori, Mitsuru; Nagai, Takeharu
作者单位：University of Osaka; University of Osaka; University of Osaka; Hokkaido University
摘要：Bioluminescence imaging has become a valuable tool in biological research, offering several advantages over fluorescence-based techniques, including the absence of phototoxicity and photobleaching, along with a higher signal-to-noise ratio. Common bioluminescence imaging methods often require the addition of an external chemical substrate (luciferin), which can result in a decrease in luminescence intensity over time and limit prolonged observations. Since the bacterial bioluminescence system ...
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作者：Vaccario, Giacomo; Xu, Shuqi; Mariani, Manuel S.; Medo, Matus
作者单位：Swiss Federal Institutes of Technology Domain; ETH Zurich; University of Zurich; University of Bern; University Hospital of Bern; University of Bern; University Hospital of Bern
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作者：Najib, Dalal; Mahmoud, Hussam; Placht, Daniel
作者单位：National Academies of Sciences, Engineering & Medicine; Colorado State University System; Colorado State University Fort Collins
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作者：Glenn, Skye; Fragasso, Alessio; Lin, Wei-Hsiang; Papagiannakis, Alexandros; Kato, Setsu; Jacobs-Wagner, Christine
作者单位：Stanford University; Stanford University; Howard Hughes Medical Institute; Stanford University; Yale University; Stanford University; Academia Sinica - Taiwan; Hiroshima University
摘要：In proliferating bacteria, growth rate is often assumed to be similar between daughter cells. However, most of our knowledge of cell growth derives from studies on symmetrically dividing bacteria. In many alpha-proteobacteria, asymmetric division is a normal part of the life cycle, with each division producing daughter cells with different sizes and fates. Here, we demonstrate that the functionally distinct swarmer and stalked daughter cells produced by the model alpha-proteobacterium Caulobac...
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作者：Abhimanyu; Longlax, Santiago Carrero; Nishiguchi, Tomoki; Ladki, Malik; Sheikh, Daanish; Martinez, Amera L.; Mace, Emily M.; Grimm, Sandra L.; Caldwell, Thaleia; Varela, Alexandra Portillo; V. Sekhar, Rajagopal; Mandalakas, Anna M.; Mlotshwa, Mandla; Ginidza, Sibuse; Cirillo, Jeffrey D.; Wallis, Robert S.; Netea, Mihai G.; van Crevel, Reinout; Coarfa, Cristian; Dinardo, Andrew R.
作者单位：Baylor College of Medicine; Baylor College Medical Hospital; Baylor College of Medicine; Columbia University; NewYork-Presbyterian Hospital; Baylor College of Medicine; Baylor College of Medicine; Baylor College of Medicine; University of Texas System; German Center for Infection Research; Leibniz Association; Forschungszentrum Borstel; University of Johannesburg; Vanderbilt University; Texas A&M University System; Texas A&M University College Station; University System of Ohio; Case Western Reserve University; Vanderbilt University; Radboud University Nijmegen; University of Bonn; University of Oxford
摘要：Severe and chronic infections, including pneumonia, sepsis, and tuberculosis (TB), induce long- lasting epigenetic changes that are associated with an increase in all- cause postinfectious morbidity and mortality. Oncology studies identified metabolic drivers of the epigenetic landscape, with the tricarboxylic acid (TCA) cycle acting as a central ylation, after infection- induced immune tolerance. The following studies demonstrate mitigated by inhibitors of cellular metabolism (rapamycin, ever...
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作者：Vijayrajratnam, Sukhithasri; Milek, Sonja; Maggi, Stefano; Ashen, Kaleigh; Ferrell, Micah; Hasanovic, Ahmet; Holgerson, Agnieszka; Kannaiah, Shanmugapriya; Singh, Manpreet; Ghosal, Debnath; Jensen, Grant J.; Vogel, Joseph P.
作者单位：Washington University (WUSTL); University of Zurich; California Institute of Technology; Brigham Young University; Michigan State University; University of Melbourne; University of Melbourne
摘要：The Legionella pneumophila Dot/Icm type IVB secretion system (T4BSS) is a large, multisubunit complex that exports a vast array of substrates into eukaryotic host cells. DotO, a distant homolog of the T4ASS ATPase VirB4, associates with the bacterial inner membrane despite lacking hydrophobic transmembrane domains. Employing a genetic approach, we found DotO's membrane association is mediated by three inner-membrane Dot/Icm components, IcmT, and a combined DotJ-DotI complex (referred to as Dot...
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作者：Xu, Jianquan; Ghanekarade, Asieh; Li, Li; Zhu, Huifeng; Yuan, Hailin; Yan, Jinsong; Simmons, David S.; Tsui, Ophelia K. C.; Wang, Xinping
作者单位：Zhejiang Sci-Tech University; State University System of Florida; University of South Florida; Hong Kong University of Science & Technology; Hong Kong University of Science & Technology
摘要：Using angle-resolved X-ray photoelectron spectroscopy, sum-frequency generation vibrational spectroscopy, contact angle measurements, and molecular dynamics simulations, we verify that the glass transition temperature (T-g) of polymer glass is lower near the free surface. However, the experimental T-g-gradients showed a linear variation with depth (z) from the free surface, while the simulated equilibrium T-g-gradients exhibited a double exponential z-dependence. In typical simulations, T-g is...
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作者：Cobb, Anna; Mohan, Aniruddh; Harper, Corey D.; Nock, Destenie; Michalek, Jeremy
作者单位：Carnegie Mellon University; Princeton University; Carnegie Mellon University; Carnegie Mellon University; Carnegie Mellon University
摘要：We assess racial disparities in the service quality of app-based ride-hailing services, like Uber and Lyft, by simulating their operations in the city of Chicago using empirical data. To generate driver cancellation rate disparities consistent with controlled experiments (up to twice as large for Black riders as for White riders), we estimate that more than 3% of drivers discriminate by race. We find that the capabilities of ride- hailing technology to rapidly rematch after a cancellation and ...
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作者：Martin, Thomas G.; Hunt, Dakota R.; Langer, Stephen J.; Tan, Yuxiao; Ebmeier, Christopher C.; Leinwand, Leslie A.
作者单位：University of Colorado System; University of Colorado Boulder; University of Colorado System; University of Colorado Boulder; University of Colorado System; University of Colorado Boulder
摘要：As ambush- hunting predators that consume large prey after long intervals of fasting, Burmese pythons evolved with unique adaptations for modulating organ structure and function. Among these is cardiac hypertrophy that develops within three days following a meal (Andersen et al., 2005, Secor, 2008), which we previously showed was initiated by circulating growth factors (Riquelme et al., 2011). Postprandial cardiac hypertrophy in pythons also rapidly regresses with subsequent fasting (Secor, 20...
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作者：Sato, Yuki; Habara, Makoto; Hanaki, Shunsuke; Masaki, Takahiro; Tomiyasu, Haruki; Miki, Yosei; Sakurai, Masashi; Morimoto, Masahiro; Kobayashi, Daigo; Miyamoto, Tatsuo; Shimada, Midori
作者单位：Yamaguchi University; Yamaguchi University; Yamaguchi University; Yamaguchi University; Nagoya University
摘要：The transcription factor E2F1 serves as a regulator of the cell cycle and promotes cell proliferation. It is highly expressed in cancer tissues and contributes to their malignant transformation. Degradation by the ubiquitin-proteasome system may help to prevent such overexpression of E2F1 and thereby to suppress carcinogenesis. A detailed understanding of the mechanisms underlying E2F1 degradation may therefore inform the development of new cancer treatments. We here identified SCF FBXW7 as a ...
