42期

最新文章

• Shedding light with harmonic radar: Unveiling the hidden impacts of streetlights on moth flight behavior AI翻译
  作者：Degen, Jacqueline; Storms, Mona; Lee, Chengfa Benjamin; Jechow, Andreas; Stoeckl, Anna Lisa; Hoelker, Franz; Jakhar, Aryan; Walter, Thomas; Walter, Stefan; Mitesser, Oliver; Hovestadt, Thomas; Degen, Tobias
  作者单位：University of Wurzburg; University of Wurzburg; Helmholtz Association; German Aerospace Centre (DLR); Leibniz Association; Leibniz Institut fur Gewasserokologie und Binnenfischerei (IGB); University of Konstanz; University of Konstanz; University of Konstanz; Free University of Berlin; Indian Institute of Science Education & Research (IISER) - Thiruvananthapuram; Brown University; Brown University; University of Wurzburg; University of Wurzburg
  摘要：One of the most dramatic changes occurring on our planet is the ever- increasing extensive use of artificial light at night, which drastically altered the environment to which nocturnal animals are adapted. Such light pollution has been identified as a driver in the dramatic insect decline of the past years. One nocturnal species group experiencing marked declines are moths, which play a key role in food webs and ecosystem services such as plant pollination. Moths can be easily monitored withi...
• Free- swimming bacteria transcriptionally respond to shear flow AI翻译
  作者：Ramachandran, Ashwin; Stone, Howard A.; Gitai, Zemer
  作者单位：Princeton University; Princeton University
  摘要：Surface- attached cells can sense and respond to shear flow, but planktonic (free- swimming) planktonic bacteria can transcriptionally respond to flow, inducing expression changes that are beneficial in flow. Specifically, we use microfluidic experiments and quantitative modeling to show that in the presence of flow, planktonic Pseudomonas aeruginosa induce shear rate-dependent genes that promote growth in low- oxygen environments. Untangling this mechanism revealed that in flow, motile P. aer...
• Impaired axon initial segment structure and function in a model of ARHGEF9 developmental and epileptic encephalopathy AI翻译
  作者：Wang, Wanqi; Williams, Damian J.; Teoh, Jia Jie; Soundararajan, Divyalakshmi; Zuberi, Aamir; Lutz, Cathleen M.; Frankel, Wayne N.; Makinson, Christopher D.
  作者单位：NewYork-Presbyterian Hospital; Columbia University; NewYork-Presbyterian Hospital; Columbia University; Columbia University; NewYork-Presbyterian Hospital; Columbia University; NewYork-Presbyterian Hospital; Jackson Laboratory
  摘要：Developmental and epileptic encephalopathies (DEE) are rare but devastating and largely intractable childhood epilepsies. Genetic variants in ARHGEF9, encoding a scaffolding protein important for the organization of the postsynaptic density of inhibitory synapses, are associated with DEE accompanied by complex neurological phenotypes. In a mouse model carrying a patient- derived ARHGEF9 variant associated with severe disease, we observed aggregation of postsynaptic proteins and loss of functio...
• Propofol- mediated loss of consciousness disrupts predictive routing and local field phase modulation of neural activity AI翻译
  作者：Xiong, Yihan (Sophy); Donoghue, Jacob A.; Lundqvist, Mikael; Mahnke, Meredith; Major, Alex James; Brown, Emery N.; Miller, Earl K.; Bastos, Andre M.
  作者单位：Vanderbilt University; Massachusetts Institute of Technology (MIT); Karolinska Institutet; Harvard University; Harvard Medical School; Harvard University Medical Affiliates; Massachusetts General Hospital; Massachusetts Institute of Technology (MIT); Vanderbilt University
  摘要：Predictive coding is a fundamental function of the cortex. The predictive routing model proposes a neurophysiological implementation for predictive coding. Predictions are fed back from the deep- layer cortex via alpha/beta (8 to 30 Hz) oscillations. They inhibit the gamma (40 to 100 Hz) and spiking that feed sensory inputs forward. Unpredicted inputs arrive in circuits unprepared by alpha/beta, resulting in enhanced gamma and spiking. To test the predictive routing model and its role in consc...
• CryoSeek: A strategy for bioentity discovery using cryoelectron microscopy AI翻译
  作者：Wang, Tongtong; Li, Zhangqiang; Xu, Kui; Huang, Wenze; Huang, Gaoxingyu; Zhang, Qiangfeng Cliff; Yan, Nieng
  作者单位：Tsinghua University; Westlake University; Westlake Laboratory; Shenzhen Medical Academy of Research & Translation (SMART); Shenzhen Bay Laboratory
  摘要：Structural biology is experiencing a paradigm shift from targeted structural determination to structure- guided discovery of previously uncharacterized bioentities. We employed cryoelectron microscopy (cryo-EM) to analyze filtered water samples collected from the Tsinghua Lotus Pond. Here, we report the structural determination and characterization of two highly similar helical fibrils, named TLP-1a and TLP-1b, each approximately 8 nm in diameter with a 15- & Aring; wide tunnel. These fibrils ...
• Native β- barrel substrates pass through two shared intermediates during folding on the BAM complex AI翻译
  作者：dos Santos, Thiago M. A.; Thomson, Benjamin D.; Marquez, Melissa D.; Pan, Lydia; Monfared, Tabasom H.; Kahne, Daniel E.
  作者单位：Northeastern University; Harvard University
  摘要：The assembly of beta- barrel proteins into membranes is mediated by the evolutionarily numerous substrates which vary considerably in size and shape. How BAM is able to efficiently fold such a diverse array of beta- barrel substrates is not clear. Here, we develop a disulfide crosslinking method to trap native substrates in vivo as they fold on BAM. By placing a cysteine within the luminal wall of the BamA barrel as well as in the substrate beta- strands, we can compare the residence time of e...
• Identification of a cell- active chikungunya virus nsP2 protease inhibitor using a covalent fragment- based screening approach AI翻译
  作者：Merten, Eric M.; Sears, John D.; Leisner, Tina M.; Hardy, P. Brian; Ghoshal, Anirban; Hossain, Mohammad Anwar; Asressu, Kesatebrhan Haile; Brown, Peter J.; Tse, Edwin G.; Stashko, Michael A.; Li, Kelin; Drouin, Jacqueline L. Norris -; Herring, Laura E.; Mordant, Angie L.; Webb, Thomas S.; Mills, Christine A.; Barker, Natalie K.; Streblow, Zachary J.; Perveen, Sumera; Arrowsmith, Cheryl H.; Counago, Rafael Miguez; Arnold, Jamie J.; Cameron, Craig E.; Streblow, Daniel N.; Moorman, Nathaniel J.; Heise, Mark T.; Willson, Timothy M.; Popov, Konstantin I.; Pearce, Kenneth H.
  作者单位：University of North Carolina; University of North Carolina Chapel Hill; University of North Carolina School of Medicine; University of North Carolina; University of North Carolina Chapel Hill; University of North Carolina School of Medicine; University of North Carolina; University of North Carolina Chapel Hill; University of North Carolina School of Medicine; University of North Carolina; University of North Carolina Chapel Hill; University of North Carolina School of Medicine; Oregon Health & Science University; University of Toronto; Structural Genomics Consortium; Universidade Estadual de Campinas; University of North Carolina; University of North Carolina Chapel Hill; University of North Carolina School of Medicine; University of North Carolina; University of North Carolina Chapel Hill; University of North Carolina School of Medicine
  摘要：Chikungunya virus (CHIKV) is a mosquito- borne alphavirus that has been responsible for numerous large- scale outbreaks in the last twenty years. Currently, there are no FDA- approved therapeutics for any alphavirus infection. CHIKV nonstructural protein 2 (nsP2), which contains a cysteine protease domain, is essential for viral replication, making it an attractive target for a drug discovery campaign. Here, we optimized a CHIKV nsP2 protease (nsP2pro) biochemical assay for the screening of a ...
• TRIM21 induces selective autophagic degradation of c- Myc and sensitizes regorafenib therapy in colorectal cancer AI翻译
  作者：Ye, Wen-Long; Huang, Long; Yang, Xiao-Qin; Wan, Shan; Gan, Wen-Juan; Yang, Yun; He, Xiao-Shun; Liu, Feng; Guo, Xin; Liu, Yi-Xuan; Hu, Guang; Li, Xiu-Ming; Shi, Wei-Yi; He, Kuang; Wu, Yue-Yue; Wu, Wen-Xin; Lu, Jun-Hou; Song, Yu; Qu, Chen-Jiang; Wu, Hua
  作者单位：Soochow University - China; Soochow University - China; Soochow University - China; Soochow University - China; Soochow University - China
  摘要：Kirsten rat sarcoma virus (KRAS) mutation is associated with malignant tumor transformation and drug resistance. However, the development of clinically effective targeted therapies for KRAS-mutant cancer has proven to be a formidable challenge. Here, we report that tripartite motif- containing protein 21 (TRIM21) functions as a target of extracellular signal- regulated kinase 2 (ERK2) in KRAS- mutant colorectal cancer (CRC), contributing to regorafenib therapy resistance. Mechanistically, TRIM...
• Deep silicification-assisted long- term preservation of structural and genomic information across biospecies: From micro to macro AI翻译
  作者：Liang, Ke; Zhou, Liang; Lei, Qi; Ruan, Ting; Zhang, Minmin; Guo, Jimin; Brinker, C. Jeffrey; Zhu, Wei
  作者单位：South China University of Technology; Guangzhou Medical University; South China Normal University; Beijing University of Chemical Technology; University of New Mexico
  摘要：The concurrent preservation of morphological, structural, and genomic attributes within biological samples is paramount for comprehensive insights into biological phenomena and disease mechanisms. However, current preservation methodologies (e.g., cryopreservation, chemical reagent fixation, and bioplasticization) exhibit limitations in simultaneously achieving these critical combined goals. To address this gap, inspired by natural fossilization, here we propose deep silicification, a room tem...