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作者：Pandey, Abhishek; Ye, Yang; Wells, Chad R.; Singer, Burton H.; Galvani, Alison P.
作者单位：Yale University; State University System of Florida; University of Florida
摘要：population, exacerbating a spectrum of other diseases and contributing significantly to morbidity and mortality overall. Recent advances in pharmaceutical interventions, tirzepatide), have shown remarkable efficacy in weight- loss. However, limited access to these medications due to high costs and insurance coverage issues restricts their utility in mitigating the obesity epidemic. We quantify the annual mortality burden directly attributable to limited access to these medications in the US. B...
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作者：Guzman-Herrador, Dolores L.; Fernandez-Gomez, Andrea; Depardieu, Florence; Bikard, David; Llosa, Matxalen
作者单位：Universidad de Cantabria; Consejo Superior de Investigaciones Cientificas (CSIC); Universidad de Cantabria; CSIC - Instituto de Biomedicina y Biotecnologia de Cantabria (IBBTEC); Pasteur Network; Universite Paris Cite; Institut Pasteur Paris
摘要：CRISPR- associated (Cas) endonucleases and their derivatives are widespread tools for the targeted genetic modification of both prokaryotic and eukaryotic genomes. A critical step of all CRISPR-Cas technologies is the delivery of the Cas endonuclease to the target cell. Here, we investigate the possibility of using bacterial conjugation to translocate Cas proteins into recipient bacteria. Conjugative relaxases are translocated through a type IV secretion system into the recipient cell, covalen...
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作者：Bao, Leo; Cao, Jingcun; Gangadharan, Lata; Huang, Difang; Lin, Chen
作者单位：Monash University; University of Hong Kong; Monash University; Chinese Academy of Sciences; Academy of Mathematics & System Sciences, CAS
摘要：Lockdowns were implemented to reduce the transmission of the COVID-19 virus. However, it is unclear how severely curtailed physical interaction shapes individual behaviors that are considered vital for socioeconomic development. In this exploratory study, we investigate the behavior of college students who experienced a strict campus- wide lockdown and those who did not. Employing a combination of experimental and survey methodologies, we elicit students' behavioral variables, which are then i...
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作者：Coquinot, Baptiste; Bocquet, Lyderic; Kavokine, Nikita
作者单位：Universite Paris Cite; Universite PSL; Ecole Normale Superieure (ENS); Sorbonne Universite; Max Planck Society; Simons Foundation; Flatiron Institute; Swiss Federal Institutes of Technology Domain; Ecole Polytechnique Federale de Lausanne
摘要：Hydraulic energy is a key component of the global energy mix, yet there exists no practical way of harvesting it at small scales, from flows with low Reynolds number. This has triggered a search for alternative hydroelectric conversion methodologies, leading to unconventional proposals based on droplet triboelectricity, water evaporation, osmotic energy, or flow-induced ionic Coulomb drag. Yet, these approaches systematically rely on ions as intermediate charge carriers, limiting the achievabl...
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作者：Windred, Daniel P.; Burns, Angus C.; Lane, Jacqueline M.; Olivier, Patrick; Rutter, Martin K.; Saxena, Richa; Phillips, Andrew J. K.; Cain, Sean W.
作者单位：Flinders University South Australia; Monash University; Harvard University; Harvard University Medical Affiliates; Brigham & Women's Hospital; Harvard University; Harvard Medical School; Harvard University; Massachusetts Institute of Technology (MIT); Broad Institute; Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; Monash University; University of Manchester; Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; Harvard University; Harvard Medical School
摘要：Light enhances or disrupts circadian rhythms, depending on the timing of exposure. Circadian disruption contributes to poor health outcomes that increase mortality risk. Whether personal light exposure predicts mortality risk has not been established. We therefore investigated whether personal day and night light, and light patterns that disrupt 62.4 +/- 7.8 y, 57% female) wore light sensors for 1 wk. Day and night light exposures were defined by factor analysis of 24- h light profiles. A comp...
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作者：Bisht, Punam; Gallagher, Michael D.; Barrasa, M. Inmaculada; Boucau, Julie; Harding, Alfred; Dejosez, Marion; Parejo, Carlos Godoy -; Bisher, Margaret E.; de Nola, Giovanni; Lytton-Jean, Abigail K. R.; Gehrke, Lee; Zwaka, Thomas P.; Jaenisch, Rudolf
作者单位：Massachusetts Institute of Technology (MIT); Whitehead Institute; Harvard University; Massachusetts Institute of Technology (MIT); Ragon Institute; Massachusetts Institute of Technology (MIT); Harvard University; Harvard Medical School; Icahn School of Medicine at Mount Sinai; Icahn School of Medicine at Mount Sinai; Massachusetts Institute of Technology (MIT); Massachusetts Institute of Technology (MIT)
摘要：Bats are tolerant to highly pathogenic viruses such as Marburg, Ebola, and Nipah, suggesting the presence of a unique immune tolerance toward viral infection. Here, we compared severe acute respiratory syndrome coronavirus 2 (SARS- CoV-2) infection of human and bat ( Rhinolophus ferrumequinum) pluripotent cells and fibroblasts. Since bat cells do not express an angiotensin- converting enzyme 2 (ACE2) receptor that allows virus infection, we transduced the human ACE2 (hA) receptor into the cell...
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作者：Fu, Afu; Luo, Zhiwen; Ziv, Tamar; Bi, Xinyu; Lulu-Shimron, Chen; Cohen-Kaplan, Victoria; Ciechanover, Aaron
作者单位：Technion Israel Institute of Technology; Rappaport Faculty of Medicine; Technion Israel Institute of Technology; Chinese Academy of Medical Sciences - Peking Union Medical College; Cancer Institute & Hospital - CAMS; Peking Union Medical College; Technion Israel Institute of Technology; Technion Israel Institute of Technology
摘要：Liquid-liquid phase separation has emerged as a crucial mechanism driving the formation of membraneless biomolecular condensates, which play important roles in numerous cellular processes. These condensates, found both in the nucleus and cytoplasm, are formed through multivalent, low- affinity interactions between various molecules. P62- containing condensates serve, among other functions, as proteolytic hubs for the ubiquitin-proteasome system. In this study, we investigated the dynamic inter...
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作者：Kim, J. J.; Simon, M.; Fleming, G.
作者单位：Northwestern University; Feinberg School of Medicine; Northwestern University; Feinberg School of Medicine; University of Chicago
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作者：Tang, Ruhang; Harasymowicz, Natalia S.; Wu, Chia-Lung; Choi, Yun-Rak; Lenz, Kristin; Oswald, Sara J.; Guilak, Farshid
作者单位：Utah System of Higher Education; University of Utah; Utah System of Higher Education; University of Utah; University of Rochester; Washington University (WUSTL); Washington University (WUSTL); Yonsei University; Yonsei University Health System
摘要：Obesity is one of the primary risk factors for osteoarthritis (OA), acting through cross talk among altered biomechanics, metabolism, adipokines, and dietary free fatty acid (FA) composition. Obesity and aging have been linked to cellular senescence in various tissues, resulting in increased local and systemic inflammation and immune dysfunction. We hypothesized that obesity and joint injury lead to cellular senescence that is typically associated with increased OA severity or with aging and t...
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作者：Teruya, Takayuki; Chen, Yung-Ju; Fukuji, Yasuhide; Kondoh, Hiroshi; Yanagida, Mitsuhiro
