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作者：Andrini, Olga; Ben Soussia, Ismail; Tardy, Philippe; Walker, Denise S.; Pena-Varas, Carlos; Ramirez, David; Gendrel, Marie; Mercier, Marine; El Mouridi, Sonia; Leclercq-Blondel, Alice; Gonzaleze, Wendy; Schafer, William R.; Jospin, Maelle; Boulin, Thomas
作者单位：Universite Claude Bernard Lyon 1; Centre National de la Recherche Scientifique (CNRS); Institut National de la Sante et de la Recherche Medicale (Inserm); MRC Laboratory Molecular Biology; Universidad de Concepcion; Institut National de la Sante et de la Recherche Medicale (Inserm); Universite PSL; Ecole Normale Superieure (ENS); Centre National de la Recherche Scientifique (CNRS); CNRS - National Institute for Biology (INSB); Universidad de Talca; KU Leuven
摘要：Two- pore domain potassium (K2P) channels play a central role in modulating cellular excitability and neuronal function. The unique structure of the selectivity filter in K2P and other potassium channels determines their ability to allow the selective passage of potassium ions across cell membranes. The nematode C. elegans has one of the largest K2P families, with 47 subunit- coding genes. This remarkable expansion has been accompanied by the evolution of atypical selectivity filter sequences ...
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作者：Guo, Ke; Kalyviotis, Konstantinos; Pantazis, Periklis; Rowlands, Christopher J.
作者单位：Imperial College London
摘要：Spontaneous Raman imaging has emerged as powerful label-free technique for investigating the molecular composition of medicines and biological specimens. Although Raman imaging can facilitate understanding of complex biological phenomena in vivo, current imaging modalities are limited in speed and sample compatibility. Here, we introduce a single-objective line-scanning light-sheet microscope, named .-OPM, which records Raman images on a timescale of minutes to seconds. To demonstrate its func...
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作者：Hill, Jennifer M.; Cai, Songlin; Carver, Michael D.; Drubin, David G.
作者单位：University of California System; University of California Berkeley
摘要：The high turgor pressure across the plasma membrane of yeasts creates a requirement for substantial force production by actin polymerization and myosin motor activity for clathrin- mediated endocytosis (CME). Endocytic internalization is severely impeded in the absence of fimbrin, an actin filament crosslinking protein called Sac6 in budding yeast. Here, we combine live- cell imaging and mathematical modeling to gain insights into the role of actin filament crosslinking proteins in force gener...
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作者：Miki, Takafumi; Okamoto, Yuji; Ueno-Umegai, Miyuki; Toyofuku, Rio; Hattori, Shun; Sakaba, Takeshi
作者单位：Akita University; Doshisha University; Akita University; University Shiga Prefecture
摘要：Synaptic- vesicle (SV) recruitment is thought to maintain reliable neurotransmitter release during high- frequency signaling. However, the mechanism underlying the SV reloading for sustained neurotransmission at central synapses remains unknown. To elucidate this, we performed direct observations of SV reloading and mobility at a single- vesicle level near the plasma membrane in cerebellar mossy fiber terminals using total internal reflection fluorescence microscopy, together with simultaneous...
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作者：Moreno, Jose Francisco Calva; Jose, George; Weaver, Yi M.; Weaver, Benjamin P.
作者单位：University of Texas System; University of Texas Southwestern Medical Center
摘要：UBR5 E3 ligase has been associated with cancer susceptibility and neuronal integrity, with functions in chromatin regulation and proteostasis. However, the functions of ubr5 within animals remain unclear due to lethality in both mammals and flies when disrupted. Using Caenorhabditis elegans, we show that UBR-5 E3 ligase is required for timely exit of stem fate and complete transition into multiple cell type descendants in an ectodermal blast lineage. Animals lacking intact UBR-5 function simul...
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作者：Chen, Jiangtian; Nouzova, Marcela; Noriega, Fernando G.; Tatar, Marc
作者单位：Brown University; Czech Academy of Sciences; Biology Centre of the Czech Academy of Sciences; State University System of Florida; Florida International University; State University System of Florida; Florida International University; University of South Bohemia Ceske Budejovice
摘要：Dietary restriction (DR) slows aging in many animals, while in some cases, the sensory signals from diet alone are sufficient to retard or accelerate lifespan. The digestive tract is a candidate location to sense nutrients, where neuropeptides secreted by enteroendocrine cells (EEC) produce systemic signals in response to food. Here, we measure how Drosophila neuropeptide F (NPF) is secreted into adult circulation by EEC and find that specific EEC differentially respond to dietary sugar and ye...
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作者：Eills, James; Mitchell, Morgan W.; Rius, Irene Marco; Tayler, Michael C. D.
作者单位：University of Barcelona; Barcelona Institute of Science & Technology; Institut de Bioenginyeria de Catalunya; Barcelona Institute of Science & Technology; Universitat Politecnica de Catalunya; Institut de Ciencies Fotoniques (ICFO); ICREA; Helmholtz Association; Research Center Julich
摘要：Hyperpolarized nuclear spins in molecules exhibit high magnetization that is unachievable by classical polarization techniques, making them widely used as sensors in physics, chemistry, and medicine. The state of a hyperpolarized material, however, is typically only studied indirectly and with partial destruction of magnetization, due to the nature of conventional detection by resonant-pickup NMR spectroscopy or imaging. Here, we establish atomic magnetometers with sub-pT sensitivity as an alt...
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作者：Gavalda-Diaz, Oriol; Emmanuel, Max; Berenov, Andrey; Marquardt, Katharina; Saiz, Eduardo; Giuliani, Finn
作者单位：Imperial College London; University of Nottingham; University of Oxford
摘要：Ultimately, brittle fracture involves breaking atomic bonds. However, we still lack a clear picture of what happens in the highly deformed region around a moving crack tip. Consequently, we still cannot link nanoscale phenomena with the macroscopic toughness of materials. The unsolved challenge is to observe the movement of the crack front at the nanoscale while extracting quantitative information. Here, we address this challenge by monitoring stable crack growth inside a transmission electron...
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作者：Posch, Bradley C.; Bush, Susan E.; Koepke, Dan F.; Schuessler, Alexandra; Anderegg, Leander L. D.; Aparecido, Luiza M. T.; Blonder, Benjamin W.; Guo, Jessica S.; Kerr, Kelly L.; Moran, Madeline E.; Cooper, Hillary F.; Doughty, Christopher E.; Gehring, Catherine A.; Whitham, Thomas G.; Allan, Gerard J.; Hultine, Kevin R.
作者单位：University of California System; University of California Berkeley; University of California System; University of California Santa Barbara; Utah System of Higher Education; University of Utah; University of Arizona; Arizona State University; Arizona State University-Tempe; Northern Arizona University; Northern Arizona University; Northern Arizona University
摘要：Increasing heatwaves are threatening forest ecosystems globally. Leaf thermal regulation and tolerance are important for plant survival during heatwaves, though the interaction between these processes and water availability is unclear. Genotypes of the widely distributed foundation tree species Populusfremontii were studied in a controlled common garden during a record summer heatwave-where air temperature exceeded 48 degrees C. When water was not limiting, all genotypes cooled leaves 2 to 5 d...
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作者：Garzon, Ramiro; Garofalo, Michela; Martelli, Maria Paola; Briesewitz, Roger; Wang, Lisheng; Fernandez-Cymering, Cecilia; Volinia, Stefano; Liu, Chang-Gong; Schnittger, Susanne; Haferlach, Torsten; Liso, Arcangelo; Diverio, Daniela; Mancini, Marco; Meloni, Giovanna; Foa, Robin; Martelli, Massimo F.; Mecucci, Cristina; Croce, Carlo M.; Falini, Brunangelo
