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作者：Westfall, Aundrea K.; Gopalan, Siddharth S.; Kay, Jarren C.; Tippetts, Trevor S.; Cervantes, Margaret B.; Lackey, Kimberly; Chowdhury, Saiful M.; Pellegrino, Mark W.; Castoe, Todd A.
作者单位：University of Texas System; University of Texas Arlington; University of Texas System; University of Texas Southwestern Medical Center; University of Alabama System; University of Alabama Tuscaloosa; University of Texas System; University of Texas Arlington
摘要：Canonical models of intestinal regeneration emphasize the critical role of the crypt stem cell niche to generate enterocytes that migrate to villus ends. Burmese pythons possess extreme intestinal regenerative capacity yet lack crypts, thus providing opportunities to identify noncanonical but potentially conserved mechanisms that expand our understanding of regenerative capacity in vertebrates, including humans. Here, we leverage single- nucleus RNA sequencing of fasted and postprandial python...
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作者：Qu, Zhiyu; Xu, Lianjie; Jiang, Fengyi; Liu, Yuan; Zhang, Wen - Bin
作者单位：Peking University; Chinese Academy of Sciences; Peking University; Peking University Shenzhen Graduate School (PKU Shenzhen); Peking University
摘要：Expanding the protein fold space beyond linear chains is of fundamental significance, yet remains largely unexplored. Herein, we report the creation of seven topological isoforms (i.e., linear, cyclic, knot, lasso, pseudorotaxane, and catenane) from a single protein fold precursor by rewiring the connectivity of secondary structure elements of the SpyTag- SpyCatcher complex and mutating the reactive residue on SpyTag to abolish the isopeptide bonding. These topological isoforms can be directly...
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作者：Muto, Yoshiharu; Yoshimura, Yasuhiro; Wu, Haojia; Panesso, Monica Chang -; Ledru, Nicolas; Woodward, Owen M.; Outeda, Patricia; Cheng, Tao; Mahjoub, Moe R.; Watnick, Terry J.; Humphreys, Benjamin D.
作者单位：Washington University (WUSTL); University System of Maryland; University of Maryland Baltimore; University System of Maryland; University of Maryland Baltimore; Washington University (WUSTL); Washington University (WUSTL)
摘要：Autosomal dominant polycystic kidney disease (ADPKD) is the most common hereditary kidney disease and causes significant morbidity, ultimately leading to kidney failure. PKD pathogenesis is characterized by complex and dynamic alterations in multiple cell types during disease progression, hampering a deeper understanding of disease mechanism and the development of therapeutic approaches. Here, we generate a single- nucleus multimodal atlas of an orthologous mouse PKD model at early, mid, and l...
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作者：Alspaugh, J. Andrew
作者单位：Duke University; Duke University; Duke University
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作者：Annapooranan, Raja; Yeerella, Ram Hemanth; Chambers, Robert J.; Li, Chenghai; Cai, Shengqiang
作者单位：University of California System; University of California San Diego; University of California System; University of California San Diego
摘要：The fabrication of pressure- sensitive adhesives (PSA) using liquid crystal elastomers (LCE) that are tolerant to substrate roughness is explored in this work. Traditional soft adhesives are designed by maintaining a balance between their cohesive strength and compliance. However, rough surfaces can significantly affect the adhesion strength of PSAs. Lowering the stiffness of the adhesive by reducing the cross- linking density or using additives can improve contact on rough surfaces. But this ...
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作者：Li, Ling; Xu, Qicheng; Jiang, Shengjing; Jing, Xin; Shen, Qirong; He, Jin-Sheng; Yang, Yunfeng; Ling, Ning
作者单位：Lanzhou University; Nanjing Agricultural University; Peking University; Peking University; Tsinghua University
摘要：Asymmetric seasonal warming trends are evident across terrestrial ecosystems, with winter temperatures rising more than summer ones. Yet, the impact of such asymmetric seasonal warming on soil microbial carbon metabolism and growth remains poorly understood. Using 18 O isotope labeling, we examined the effects of a decade- long experimental seasonal warming on microbial carbon use efficiency (CUE) and growth in alpine grassland ecosystems. Moreover, the quantitative stable isotope probing with...
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作者：Goda, Makoto; Shribak, Michael; Ikeda, Zenki; Okada, Naobumi; Tani, Tomomi; Goshima, Gohta; Oldenbourg, Rudolf; Kimura, Akatsuki
作者单位：Marine Biological Laboratory - Woods Hole; Hamamatsu University School of Medicine; Nagoya University; Research Organization of Information & Systems (ROIS); National Institute of Genetics (NIG) - Japan; Graduate University for Advanced Studies - Japan; National Institute of Advanced Industrial Science & Technology (AIST)
摘要：Organelles in cells are appropriately positioned, despite crowding in the cytoplasm. However, our understanding of the force required to move large organelles, such the nucleus, inside the cytoplasm is limited, in part owing to a lack of accurate methods for measurement. We devised a method to apply forces to the nucleus of living Caenorhabditis elegans embryos to measure the force generated inside the cell. We used centrifuge polarizing microscope to apply centrifugal force and orientation- i...
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作者：Ntoumanis, Ioannis; Sheronova, Julia; Davydova, Alina; Dolgaleva, Maria; Jaaskelainen, Iiro P.; Kosonogov, Vladimir; Shestakova, Anna N.; Klucharev, Vasily
作者单位：HSE University (National Research University Higher School of Economics); Cook Children's Medical Center; University of Texas System; University of Texas Arlington; HSE University (National Research University Higher School of Economics)
摘要：Effective health promotion may benefit from understanding how persuasion emerges. While earlier research has identified brain regions implicated in persuasion, these studies often relied on event- related analyses and frequently simplified persuasive communications. The present study investigates the neural basis of valuation change induced by a persuasive healthy eating call, employing naturalistic stimuli. Fifty healthy participants performed two blocks of a bidding task, in which they had t...
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作者：Chang, Dennis; Caterina, Michael J.
作者单位：Johns Hopkins University; Johns Hopkins University; Johns Hopkins University; Johns Hopkins University
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作者：Renzer, Galit; Ribeiro, Ingrid de Almeida; Guo, Hao - Bo; Nowoiskyd, Janine Froehlich-; Berry, Rajiv J.; Bonn, Mischa; Molinero, Valeria; Meister, Konrad
作者单位：Max Planck Society; Utah System of Higher Education; University of Utah; United States Department of Defense; United States Air Force; US Air Force Research Laboratory; Max Planck Society; Boise State University
摘要：Bacterial ice nucleating proteins (INPs) are exceptionally effective in promoting the kinetically hindered transition of water to ice. Their efficiency relies on the assembly of INPs into large functional aggregates, with the size of ice nucleation sites determining activity. Experimental freezing spectra have revealed two distinct, defined aggregate sizes, typically classified as class A and C ice nucleators (INs). Despite the importance of INPs and years of extensive research, the precise nu...
