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作者：Choi, Alexander A.; Zhou, Coral Y.; Tabo, Ayana; Heald, Rebecca; Xu, Ke
作者单位：University of California System; University of California Berkeley; University of California System; University of California Berkeley
摘要：The living cell creates a unique internal molecular environment that is challenging to characterize. By combining single- molecule displacement/diffusivity mapping (SMdM) with physiologically active extracts prepared from Xenopus laevis eggs, we sought to elucidate molecular properties of the cytoplasm. Quantification of the diffusion coefficients of 15 diverse proteins in extract showed that, compared to in water, negatively charged proteins diffused similar to 50% slower, while diffusion of ...
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作者：Creel, Roger; Guimond, Julia; Jones, Benjamin M.; Nielsen, David M.; Bristol, Emily; Tweedie, Craig E.; Overduin, Pier Paul
作者单位：Woods Hole Oceanographic Institution; Woods Hole Oceanographic Institution; University of Alaska System; University of Alaska Fairbanks; Max Planck Society; University of Hamburg; University of Texas System; University of Texas Austin; University of Texas System; University of Texas El Paso; University of Texas System; University of Texas El Paso; University of Texas System; University of Texas El Paso; Helmholtz Association; Alfred Wegener Institute, Helmholtz Centre for Polar & Marine Research
摘要：Arctic shorelines are vulnerable to climate change impacts as sea level rises, permafrost thaws, storms intensify, and sea ice thins. Seventy-five years of aerial and satellite observations have established coastal erosion as an increasing Arctic hazard. However, other hazards at play-for instance, the cumulative impact that sea-level rise and permafrost thaw subsidence will have on permafrost shorelines-have received less attention, preventing assessments of these processes' impacts compared ...
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作者：Lin, Yu; Huang, Danji; Wen, Qunlei; Yang, Ruoou; Chen, Bowen; Shen, Yi; Liu, Youwen; Fang, Jiakun; Li, Huiqiao; Zhai, Tianyou
作者单位：Huazhong University of Science & Technology; Huazhong University of Science & Technology; China University of Geosciences; Huazhong University of Science & Technology; Huazhong University of Science & Technology
摘要：The dissolution of active atoms under operating potential will lead to a decline in their oxygen evolution reaction (OER) performance, thus preventing the current highly active catalysts from being practically applicable in industrial water electrolysis. Here, we propose a sequential leaching strategy to utilize the dynamic restructuring and enhance the chemical bond strength for highly active and stable OER. Modeling on nickel- iron sulfides (NiFe-S), we introduced and utilized foreign Mo dop...
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作者：Ravanfar, Raheleh; Sheng, Yuling; Gray, Harry B.; Winkler, Jay R.
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作者：Takaki, Ryota; Thirumalai, D.
作者单位：Max Planck Society; University of Texas System; University of Texas Austin; University of Texas System; University of Texas Austin
摘要：Understanding the biophysical basis of protein aggregation is important in biology because of the potential link to several misfolding diseases. Although experiments have shown that protein aggregates adopt a variety of morphologies, the dynamics of their formation are less well characterized. Here, we introduce a minimal model to explore the dependence of the aggregation dynamics on the structural and sequence features of the monomers. Using simulations, we demonstrate that sequence complexit...
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作者：Wu, Yali; Wang, Shaonan; Guo, Zhicong; Sun, Min; Xu, Zhen; Du, Yu; Zhu, Fahui; Su, Yajuan; Xu, Zhou; Xu, Yi; Gong, Xu; Fang, Ruan; Hu, Jiaojiao; Peng, Yan; Ding, Zhaowen; Liu, Cong; Li, Ang; He, Weiwei
作者单位：East China University of Science & Technology; Chinese Academy of Sciences; Shanghai Institute of Organic Chemistry, CAS; University of Chinese Academy of Sciences, CAS; Chinese Academy of Sciences; Shanghai Institute of Organic Chemistry, CAS
摘要：Autophagy is a conserved catabolic process crucial for maintaining cellular homeostasis and has emerged as a promising therapeutic target for many diseases. Mechanistically novel small- molecule autophagy regulators are highly desirable from a pharmacological point of view. Here, we report the macroautophagy- inhibitory effect of hapalindole Q, a member of the structurally intriguing but biologically understudied hapalindole family of indole terpenoids. This compound promotes the noncanonical ...
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作者：Aimutis, William R.; Shirwaiker, Rohan
作者单位：North Carolina State University; North Carolina State University; North Carolina State University
摘要：Plant- based proteins are promoted as nutritious, environ mentallysafe, and a keyelementforfood security. Numerous studies using environmental life cycle assessments confirmed plant- based meat alternatives are more environmentally favorable than animal- derived meat. However, most missed other factors from protein extraction and concentration that contribute to environmental impact (both positive and negative). Furthermore, few studies examined the entire environmental impact because they ove...
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作者：Yan, Luyao; Cao, Xiaodan; Wang, Lijuan; Chen, Jie; Sancar, Aziz; Zhong, Dongping
作者单位：Shanghai Jiao Tong University; University of North Carolina; University of North Carolina Chapel Hill; University of North Carolina School of Medicine
摘要：Photolyase and cryptochrome belong to a group of structurally similar flavoproteins but with two distinct functions of DNA repair as a photoenzyme and signal transduction as a photoreceptor, respectively, under blue- light illumination. Here, we studied a recently discovered bifunctional Chlamydomonas reinhardtii cryptochrome (CraCRY) with focus on its repair of UV- induced pyrimidine- pyrimidone (6- 4) photoproduct (6-4PP). We used femtosecond spectroscopy and site- directed mutagenesis to ma...
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作者：Zanders, Sarah E.; Smith, Gerald R.
作者单位：Stowers Institute for Medical Research; University of Kansas; University of Kansas Medical Center; Fred Hutchinson Cancer Center
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作者：Filho, Paulo Meo -; Agudelo, John F. Ramirez -; Kebreab, Ermias
作者单位：University of California System; University of California Davis
摘要：The ruminant livestock sector considerably contributes to global greenhouse gas emissions. This study investigates the effectiveness of pelleted bromoform- containing seaweed ( Asparagopsis taxiformis) (Brominata) as an enteric methane (CH4) inhibitor in grazing beef cattle. The primary objective was to assess the impact of this antimethanogenic additive on enteric CH4 emissions under real- world farm conditions. Twenty- four beef steers, crossbreeds of Wagyu and Angus, with an average livewei...
