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作者：Kanai, Ryuta; Tsunekawa, Naoki; Cornelius, Flemming; Vilsen, Bente; Toyoshima, Chikashi
作者单位：University of Tokyo; Aarhus University
摘要：Palytoxin (PTX), a potent marine toxin, has long been known to transform Na+,K+- ATPase (NKA), an indispensable ion pump, into a nonselective cation channel. It has been postulated that PTX takes control of the two gates on either side of a channel-like pore. These gates normally open and close alternately, synchronized with chemical events, never opening simultaneously. A critical question is whether palytoxin takes over the control of the two gates or creates a new pathway. Here, we present ...
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作者：Rennich, Emily; Sung, Suk Hyun; Agarwal, Nishkarsh; Gates, Maya; Kerns, Robert; Hovden, Robert; El Baggari, Ismail
作者单位：Harvard University; University of Michigan System; University of Michigan; University of Michigan System; University of Michigan; University of Michigan System; University of Michigan; University of Michigan System; University of Michigan
摘要：Cryogenic transmission electron microscopy has revolutionized structural biology and materials science. To image below liquid nitrogen temperatures, various liquid helium stages have been constructed but have proven to be complex and unstable, making high-resolution imaging challenging. This problem is even more pronounced in side-entry specimen holders common on modern transmission electron microscopes. Here, we introduce an ultracold liquid helium transmission electron microscope side-entry ...
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作者：Cionca, Alexandre; Chan, Chun Hei Michael; Van De Ville, Dimitrivan
作者单位：Swiss Federal Institutes of Technology Domain; Ecole Polytechnique Federale de Lausanne; University of Geneva
摘要：Community structure is a key feature omnipresent in real-world network data. Plethora of methods have been proposed to reveal subsets of densely interconnected nodes using criteria such as the modularity index. These approaches have been successful for undirected graphs but directed edge information has not yet been dealt with in a satisfactory way. Here, we revisit the concept of directed communities as a mapping between sending and receiving communities. This translates into a definition tha...
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作者：Cummins, Jamie; Elson, Malte; Hussey, Ian
作者单位：University of Bern; University of Oxford
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作者：Michaelsen, Patrik; Sundstrom, Aksel; Jagers, Sverker C.
作者单位：University of Gothenburg; Linkoping University
摘要：Rapid global expansion of protected areas is critical for safeguarding biodiversity but depends on political action for successful implementation. Following widespread ratification of the Kunming-Montreal Global Biodiversity Framework, an unprecedented increase in area-based conservation is required to reach its target of conserving 30% of land, waters, and seas by 2030. These expansions prompt difficult trade-offs between conservation, social, and economic interests. A key factor in securing ...
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作者：Sabatinia, Francesca; Albertina, Fauzia; Dohertya, Brenda; Monicoa, Letizia; Rosia, Francesca; Butic, David; Romania, Aldo; Peccia, Antonio; Abated, Nicodemo; Sileod, Maria; Amodiod, Antonio Minervino; Masinid, Nicola; Pizzimentie, Silvia; Deganoe, Ilaria; Modugnoe, Francesca; Campanellaf, Beatrice; Legnaiolif, Stefano; Cartechinia, Laura
作者单位：University of Milano-Bicocca; University of Basilicata; University of Naples Federico II; University of Perugia; University of Perugia; University of Pisa; Consiglio Nazionale delle Ricerche (CNR); Isituto di Chimica dei Composti Organometallici (ICCOM-CNR)
摘要：Street art murals are increasingly recognized as valuable contemporary artworks, often attaining significant artistic, historical, and social importance. As these murals become integral parts of cultural heritage, finding efficient strategies for their conservation is crucial. However, their typical large surface areas, heterogeneous materials, and high variability in exposure to environmental and pollution factors pose significant challenges in establishing appropriate analytical strategies t...
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作者：Mahajan, Shivansh; Pall, Ashley E.; Li, Yancheng E.; Stemmler, Timothy L.; Rees, Douglas C.; Clemons Jr, William M.
作者单位：California Institute of Technology; Wayne State University; Howard Hughes Medical Institute; Chan Zuckerberg Initiative (CZI); Medical University of South Carolina
摘要：Arsenite (AsIII) is toxic to all organisms due to its ability to tightly bind exposed thiols within cells. An important AsIII resistance mechanism in prokaryotes involves proteins encoded by the ars operon. A central component of the ars operon in many bacteria is the cytoplasmic ATPase, ArsA, which orchestrates a series of nucleotide-dependent handoffs, starting with the capture of AsIII by the ArsD metallochaperone and culminating in its removal from the cell by the ArsB efflux pump. Althoug...
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作者：Kuo, Chia-Ling; Liu, Peiran; Drouard, Gabin; Vuoksimaa, Eero; Kaprio, Jaakko; Ollikainen, Miina; Chen, Zhiduo; Pilling, Luke C.; Atkins, Janice L.; Fortinsky, Richard H.; Kuchel, George A.; Diniz, Breno S.
作者单位：University of Connecticut; University of Connecticut; University of Helsinki; University of Exeter; University of Connecticut
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作者：Junod, Samuel L.; Rush, Coby; Tingey, Mark; Yang, Weidong
作者单位：Pennsylvania Commonwealth System of Higher Education (PCSHE); Temple University
摘要：Efficient gene expression depends on the tightly regulated export of messenger RNA (mRNA) through nuclear pore complexes (NPCs), which are densely modified by O-linked N-acetylglucosamine (O-GlcNAc). Although dysregulated O-GlcNAcylation has been linked to a variety of human diseases, the precise distribution of O-GlcNAc within the NPC and its effects on mRNA export remain poorly understood. Here, we combined single-point edge-excitation subdiffraction (SPEED) microscopy with stochastic optica...
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作者：Mizutani, Natsuki; Yonezawa, Yasushige; Nakagawa, Atsushi; Okamura, Yasushi
作者单位：University of Osaka; University of Osaka; Japan Society for the Promotion of Science; University of Osaka; Kindai University (Kinki University); University of Osaka; Jichi Medical University
摘要：Voltage-sensing phosphatase (VSP) comprises a voltage sensor domain (VSD) and a cytoplasmic catalytic region (CCR), achieving a unique electrochemical signal conversion. Previous studies suggest that phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), a membrane phospholipid known to be critical for activities of diverse voltage-gated ion channels, associates with a linker connecting the VSD with the CCR of VSP and regulates VSD-CCR coupling. However, the details of PI(4,5)P2 interaction with t...
